2022
Kidney-Targeted Renalase Agonist Prevents Cisplatin-Induced Chronic Kidney Disease by Inhibiting Regulated Necrosis and Inflammation
Guo X, Xu L, Velazquez H, Chen TM, Williams RM, Heller DA, Burtness B, Safirstein R, Desir GV. Kidney-Targeted Renalase Agonist Prevents Cisplatin-Induced Chronic Kidney Disease by Inhibiting Regulated Necrosis and Inflammation. Journal Of The American Society Of Nephrology 2022, 33: 342-356. PMID: 34921111, PMCID: PMC8819981, DOI: 10.1681/asn.2021040439.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntineoplastic AgentsCell LineCisplatinCreatinineDisease Models, AnimalGene ExpressionGlomerular Filtration RateHepatitis A Virus Cellular Receptor 1HumansKidneyMiceMice, Inbred C57BLMice, KnockoutMonoamine OxidaseNanocapsulesPeptidesRenal Insufficiency, ChronicConceptsRenal proximal tubulesSingle-cell RNA sequencing analysisMesoscale nanoparticlesFirst doseCisplatin chemotherapyProximal tubulesAgonist peptideInduced Chronic Kidney DiseaseGenetic deletionNeck squamous cell carcinomaRNA sequencing analysisCisplatin-induced AKIKidney-targeted deliveryChronic kidney diseaseDevelopment of CKDSquamous cell carcinomaAdministration of cisplatinPlasma renalaseAdvanced headCell carcinomaInflammatory cytokinesKidney diseasePlasma creatinineSystemic administrationRegulated necrosis
2017
The serum protein renalase reduces injury in experimental pancreatitis
Kolodecik TR, Reed AM, Date K, Shugrue C, Patel V, Chung SL, Desir GV, Gorelick FS. The serum protein renalase reduces injury in experimental pancreatitis. Journal Of Biological Chemistry 2017, 292: 21047-21059. PMID: 29042438, PMCID: PMC5743078, DOI: 10.1074/jbc.m117.789776.Peer-Reviewed Original ResearchMeSH KeywordsAcinar CellsAnimalsAnti-Inflammatory Agents, Non-SteroidalBiomarkersCalcium SignalingCarbacholCell LineCeruletideEnzyme ActivationFluorescent Antibody Technique, IndirectGene Expression Regulation, EnzymologicHumansHypertensionLigandsMembrane Transport ModulatorsMiceMice, KnockoutMonoamine OxidasePancreasPancreatitisPlasma Membrane Calcium-Transporting ATPasesRecombinant Fusion ProteinsTaurolithocholic AcidConceptsRecombinant human renalaseAcute pancreatitisAcute injuryCell injuryAcinar cell injuryHuman acinar cellsCytosolic calcium levelsPlasma membrane calcium ATPasePancreatitis onsetIschemic injuryWT micePathological increaseHistological changesProtective effectSevere diseaseMurine modelMembrane calcium ATPasePancreatitisCalcium levelsExperimental pancreatitisBile acidsTissue damageRenalaseInjuryCerulein model
2015
Identification of a Receptor for Extracellular Renalase
Wang L, Velazquez H, Chang J, Safirstein R, Desir GV. Identification of a Receptor for Extracellular Renalase. PLOS ONE 2015, 10: e0122932. PMID: 25906147, PMCID: PMC4407985, DOI: 10.1371/journal.pone.0122932.Peer-Reviewed Original ResearchMeSH KeywordsAcute Kidney InjuryCell LineCytoprotectionDown-RegulationEpidermal Growth FactorEssential HypertensionHumansHypertensionMitogen-Activated Protein KinasesMonoamine OxidasePlasma Membrane Calcium-Transporting ATPasesPolymorphism, Single NucleotideProtein Interaction Domains and MotifsSignal TransductionConceptsAcute kidney injuryIntrinsic enzymatic activityMAPK signalingExtracellular renalaseHuman proximal tubular cell line HK-2Enzymatic activitySingle nucleotide gene polymorphismsAcute ischemic kidneyWild-type miceProtein-protein interactionsPlasma membrane ATPaseKidney injuryIschemic kidneyEssential hypertensionIschemic injuryCardiac injuryPMCA4b expressionRecombinant renalaseEpidermal growth factorType miceCardiac hypertrophyHK-2Control studyGene polymorphismsCell signaling
2014
Renalase Prevents AKI Independent of Amine Oxidase Activity
Wang L, Velazquez H, Moeckel G, Chang J, Ham A, Lee HT, Safirstein R, Desir GV. Renalase Prevents AKI Independent of Amine Oxidase Activity. Journal Of The American Society Of Nephrology 2014, 25: 1226-1235. PMID: 24511138, PMCID: PMC4033373, DOI: 10.1681/asn.2013060665.Peer-Reviewed Original ResearchConceptsIschemic injuryCatecholamine levelsRecombinant renalaseAmine oxidase activityHuman proximal tubular cellsCisplatin-induced AKITreatment of AKIWild-type miceHK-2 cellsProximal tubular cellsOxidase activityKidney injuryRenal injuryC-Jun N-terminal kinaseExtracellular signal-regulated kinaseP38 mitogen-activated protein kinaseToxic injuryRenalase proteinTubular cellsSignal-regulated kinaseIntracellular signaling cascadesRenalaseInjuryMitogen-activated protein kinaseN-terminal kinase
1992
Isolation of putative voltage-gated epithelial K-channel isoforms from rabbit kidney and LLC-PK1 cells
Desir GV, Hamlin HA, Puente E, Reilly RF, Hildebrandt F, Igarashi P. Isolation of putative voltage-gated epithelial K-channel isoforms from rabbit kidney and LLC-PK1 cells. American Journal Of Physiology 1992, 262: f151-f157. PMID: 1733291, DOI: 10.1152/ajprenal.1992.262.1.f151.Peer-Reviewed Original ResearchConceptsDeduced amino acid sequenceAmino acid sequenceAcid sequenceVoltage-gated K channelsRabbit genomic DNAPutative transmembrane segmentsShaker-like genesPutative voltage sensorShaker gene familyVoltage-gated potassium channelsGene familyShaker proteinRenal epithelial cellsTransmembrane segmentsSequence similarityRabbit cDNAEpithelial cell lineSouthern analysisProtein sequencesLLC-PK1 cellsDifferent genesGenomic DNAKidney cDNACDNAS4 segment