2017
Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]+ ion in exposed human cells
Wisnewski AV, Liu J, Redlich CA, Nassar AF. Polymerization of hexamethylene diisocyanate in solution and a 260.23 m/z [M+H]+ ion in exposed human cells. Analytical Biochemistry 2017, 543: 21-29. PMID: 29175138, PMCID: PMC5826792, DOI: 10.1016/j.ab.2017.11.017.Peer-Reviewed Original ResearchMeSH KeywordsCells, CulturedChromatography, LiquidHumansIonsIsocyanatesMass SpectrometryMolecular StructurePolymerizationSolutionsConceptsHexamethylene diisocyanateCollision-induced dissociation (CID) fragmentation patternsNucleophilic additionImportant industrial chemicalPrimary aminesFragmentation patternsO groupLC-MS/MSLC-MS/MS analysisLC-MSReaction productsIonsMS analysisFormula CIndustrial chemicalsDiisocyanatePolymerizationPolymersReaction targetWaterSolutionAminesDefinitive characterizationMoleculesDimersMass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol
Hettick JM, Law BF, Lin CC, Wisnewski AV, Siegel PD. Mass spectrometry-based analysis of murine bronchoalveolar lavage fluid following respiratory exposure to 4,4'-methylene diphenyl diisocyanate aerosol. Xenobiotica 2017, 48: 626-636. PMID: 28629263, PMCID: PMC5864557, DOI: 10.1080/00498254.2017.1344791.Peer-Reviewed Original ResearchConceptsLavage fluidOccupational allergic respiratory diseaseBALB/c mouse modelMurine bronchoalveolar lavage fluidDiisocyanate-induced asthmaBronchoalveolar lavage fluidAllergic respiratory diseasesBronchoalveolar lavage samplesDiphenyl diisocyanate aerosolsLabel-free quantitative proteomic strategyMDI conjugationWestern blot analysisHealth burdenLavage samplesRespiratory diseaseAerosol exposureMouse modelDisease pathogenesisRespiratory exposureCausative agentBlot analysisCytochrome P450Argininosuccinate synthaseQuantitative proteomic strategyCellular fractionsReaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy
Wisnewski AV, Kanyo J, Asher J, Goodrich JA, Barnett G, Patrylak L, Liu J, Redlich CA, Nassar AF. Reaction products of hexamethylene diisocyanate vapors with “self” molecules in the airways of rabbits exposed via tracheostomy. Xenobiotica 2017, 48: 488-497. PMID: 28489470, PMCID: PMC5863241, DOI: 10.1080/00498254.2017.1329569.Peer-Reviewed Original ResearchConceptsCollision-induced dissociation (CID) fragmentation patternsReaction productsLower airwaysChemical reactivityLow molecular weight fractionWeight fractionHigh molecular weight fractionAliphatic diisocyanateMolecular weight fractionFragmentation patternsHexamethylenediisocyanateLC-MSHDI vaporAirways of rabbitsBronchoalveolar lavage fluidRabbit bronchoalveolar lavage fluidMoleculesDiisocyanate vaporsMs. 4Albumin. 5Occupational asthmaReactivityBAL fluidAsthma pathogenesisLavage fluid
2016
UPLC–MS for metabolomics: a giant step forward in support of pharmaceutical research
Nassar AF, Wu T, Nassar SF, Wisnewski AV. UPLC–MS for metabolomics: a giant step forward in support of pharmaceutical research. Drug Discovery Today 2016, 22: 463-470. PMID: 27919805, PMCID: PMC5721520, DOI: 10.1016/j.drudis.2016.11.020.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsChromatography, LiquidDrug DiscoveryHumansMass SpectrometryMetabolomicsPharmaceutical ResearchProgress in automation of mass cytometry barcoding for drug development
Nassar AF, Wisnewski AV, Raddassi K. Progress in automation of mass cytometry barcoding for drug development. Bioanalysis 2016, 8: 1429-1435. PMID: 27323800, DOI: 10.4155/bio-2016-0135.Peer-Reviewed Original ResearchMass cytometry moving forward in support of clinical research: advantages and considerations
Nassar AF, Wisnewski AV, Raddassi K. Mass cytometry moving forward in support of clinical research: advantages and considerations. Bioanalysis 2016, 8: 255-257. PMID: 26847541, DOI: 10.4155/bio.15.257.Peer-Reviewed Original Research