2015
Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ
Wisnewski AV, Liu J. Immunochemical detection of the occupational allergen, methylene diphenyl diisocyanate (MDI), in situ. Journal Of Immunological Methods 2015, 429: 60-65. PMID: 26690039, PMCID: PMC4753098, DOI: 10.1016/j.jim.2015.12.008.Peer-Reviewed Original ResearchConceptsKeyhole limpet hemocyaninFreund's adjuvantDiisocyanate-induced asthmaComplete Freund's adjuvantIncomplete Freund's adjuvantLung tissue sectionsFormalin-fixed paraffinOccupational asthmaLower airwaysBooster injectionOccupational allergensAirway cellsAlveolar macrophagesLimpet hemocyaninAdjuvantHealth effectsTissue distributionTissue samplesTissue sectionsAsthmaDot blot analysisFurther studiesImmunochemical studiesBlot analysisEpithelial regions
2013
Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate
Wisnewski AV, Liu J. Molecular determinants of humoral immune specificity for the occupational allergen, methylene diphenyl diisocyanate. Molecular Immunology 2013, 54: 233-237. PMID: 23295252, PMCID: PMC3563841, DOI: 10.1016/j.molimm.2012.11.017.Peer-Reviewed Original ResearchConceptsEpitope specificityAutologous serum proteinsLow molecular weight chemicalsOccupational asthmaOccupational allergensMolecular determinantsDisease pathogenesisSecreting clonesCDR3 regionWestern blotGene usageMonoclonal antibodiesMajor causeWeight chemicalsIgG1 mAbGermline gene usageHuman albuminImmune specificityMAbsSerum proteinsELISADiagnostic reagentsUseful diagnostic reagentHybridomasSomatic hypermutation
2012
Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin
Wisnewski AV, Mhike M, Hettick JM, Liu J, Siegel PD. Hexamethylene diisocyanate (HDI) vapor reactivity with glutathione and subsequent transfer to human albumin. Toxicology In Vitro 2012, 27: 662-671. PMID: 23178851, PMCID: PMC3587365, DOI: 10.1016/j.tiv.2012.11.013.Peer-Reviewed Original ResearchConceptsHDI vaporCommon occupational allergensRespiratory tract exposureHuman albuminLevels of GSHReaction productsGSH concentrationVapor exposureIntra-molecular cyclizationClinical responseOccupational allergensAsthma pathogenesisPolyclonal rabbit serumAirway fluidSerologic assaysLower GSH concentrationsVivo reactivityRabbit serumPotential mechanismsGlutathione reactivityMass spectrometryExposureReactivityGSH solutionKey step
2009
Immune Responses Triggered by Skin Exposure to the Occupational Allergen, Methylene-Diphenyl Diisocyanate (MDI).
Wisnewski A, Liu J, Herrick C, Redlich C. Immune Responses Triggered by Skin Exposure to the Occupational Allergen, Methylene-Diphenyl Diisocyanate (MDI). 2009, a1645. DOI: 10.1164/ajrccm-conference.2009.179.1_meetingabstracts.a1645.Peer-Reviewed Original Research