2021
Mechanisms, Genetics, and Pathophysiology
Maestrelli P, Wisnewski A, Carlsten C, Munoz X, Tsui H, Vanoirbeek J, Malo J, Bernstein D. Mechanisms, Genetics, and Pathophysiology. 2021, 35-54. DOI: 10.1201/9781003000624-4.Peer-Reviewed Original ResearchOccupational asthmaAirway inflammationDevelopment of OASensitizer-induced occupational asthmaType of OAChemical respiratory allergyIgE-dependent hypersensitivityIrritant-induced asthmaIgE-mediated mechanismNeutrophilic airway inflammationIgE-mediated responsesWork-related symptomsPossible pathogenic mechanismsPathways of inflammationStimulation of cholinergicPathophysiology of diseaseHMW agentsInflammatory markersAirflow limitationBronchial hyperresponsivenessImmunologic sensitizationSensory nervesSensitized subjectsIgE antibodiesLMW agents
2015
Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation
Wisnewski AV, Liu J, Colangelo CM. Glutathione Reaction Products with a Chemical Allergen, Methylene-diphenyl Diisocyanate, Stimulate Alternative Macrophage Activation and Eosinophilic Airway Inflammation. Chemical Research In Toxicology 2015, 28: 729-737. PMID: 25635619, PMCID: PMC4667722, DOI: 10.1021/tx5005002.Peer-Reviewed Original ResearchConceptsEosinophilic inflammatory responseAlternative macrophage activationInflammatory responseChemical allergensMacrophage activationAirway eosinophil numbersEosinophilic airway inflammationInnate immune responseGreater inflammatory responseAirway levelsMDI conjugationAirway inflammationOccupational asthmaIL-12MDI exposureAsthma pathogenesisEosinophil numbersNaïve miceAirway fluidRespiratory tractImmune responseMucus productionSkin exposureMDI conjugatesVivo evidence
2010
Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate
Wisnewski AV, Liu J, Redlich CA. Antigenic changes in human albumin caused by reactivity with the occupational allergen diphenylmethane diisocyanate. Analytical Biochemistry 2010, 400: 251-258. PMID: 20123080, PMCID: PMC3106273, DOI: 10.1016/j.ab.2010.01.037.Peer-Reviewed Original ResearchConceptsHigh-performance liquid chromatographyDiphenylmethane diisocyanateConjugation sitesReaction productsCross-linking agentTandem mass spectrometryPolyurethane productionMass spectrometryMDI-exposed workersCause of asthmaHuman albuminSerum immunoglobulin GLiquid chromatographyReaction specificityDiisocyanateSecond lysineMDI conjugationAirway inflammationAnalytical methodSelf proteinsProteomic methodologiesEpitope specificityAntigenic changesClinical researchConjugates
2003
Differential roles for CD4 and CD8 T cells after diisocyanate sensitization: Genetic control of TH2-induced lung inflammation
Herrick CA, Das J, Xu L, Wisnewski AV, Redlich CA, Bottomly K. Differential roles for CD4 and CD8 T cells after diisocyanate sensitization: Genetic control of TH2-induced lung inflammation. Journal Of Allergy And Clinical Immunology 2003, 111: 1087-1094. PMID: 12743574, DOI: 10.1067/mai.2003.1413.Peer-Reviewed Original ResearchConceptsContact hypersensitivityT cellsAirway eosinophiliaAirway inflammationBALB/c miceDiisocyanate-induced asthmaCD8 T cellsLung inflammatory responseMajor effector cellsIL-13 productionMeans of inhalationNovel mouse modelEpicutaneous exposureAtopic asthmaLung inflammationMucus hypersecretionOccupational asthmaEpicutaneous sensitizationEffector cellsCytokine productionC miceIL-5Serum antibodiesInflammatory responseC57BL/10 mice
1999
The Immunology and Prevention of Isocyanate Asthma: A Model for Low Molecular Weight Asthma
Redlich C, Cain H, Wisnewski A. The Immunology and Prevention of Isocyanate Asthma: A Model for Low Molecular Weight Asthma. Seminars In Respiratory And Critical Care Medicine 1999, 20: 591-599. DOI: 10.1055/s-2007-1009478.Peer-Reviewed Reviews, Practice Guidelines, Standards, and Consensus StatementsIsocyanate-conjugated human lung epithelial cell proteins: A link between exposure and asthma?
Wisnewski A, Lemus R, Karol M, Redlich C. Isocyanate-conjugated human lung epithelial cell proteins: A link between exposure and asthma? Journal Of Allergy And Clinical Immunology 1999, 104: 341-347. PMID: 10452755, DOI: 10.1016/s0091-6749(99)70377-5.Peer-Reviewed Original ResearchConceptsIsocyanate-induced asthmaHuman lung epithelial cell lineEpithelial cell proteinsLung epithelial cell lineEpithelial cell lineLymphocyte proliferationProliferation of PBMCCell linesAirway inflammationAtopic subjectsNonasthmatic individualsHDI exposureAirway epitheliumImmunostimulatory potentialAsthmaCell proteinsWestern blottingPotential roleProliferationExposureHDI concentrationCross-linking chemicalsCell polypeptidesConjugation patternSubjects