2022
Culture-Independent Detection and Identification of Leptospira Serovars
Matthias M, Lubar A, Acharige S, Chaiboonma K, Pilau N, Marroquin A, Jayasundara D, Agampodi S, Vinetz J. Culture-Independent Detection and Identification of Leptospira Serovars. Microbiology Spectrum 2022, 10: e02475-22. PMID: 36445143, PMCID: PMC9769591, DOI: 10.1128/spectrum.02475-22.Peer-Reviewed Original ResearchConceptsPublic health importanceProspective studyFatal diseaseHealth importanceOutbreak investigationSequence typingDog urine samplesCulture-independent assaysEnvironmental surveillance studyMultilocus sequence typingPulsed-field gel electrophoresisSurveillance studyGroup IIHuman leptospirosisPrognostic testSpecific real-time qPCRClinical settingGroup ILeptospira serovarsReal-time qPCRDiagnostic testsSerum samplesUrine samplesDiagnostic specificityLeptospirosisDemographic, exposure, clinical, biochemical and diagnostic data of febrile patients recruited for the largest field study on leptospirosis in Sri Lanka
Agampodi S, Warnasekara J, Siribaddana S, Kularatna S, Gamage C, Jayasundara D, Senevirathna I, Srimantha S, Kappagoda C, Weerawansa P, Pilapitiya S, Lokunarangoda N, Sarathchandra C, Senanayake H, Matthias M, Vinetz J. Demographic, exposure, clinical, biochemical and diagnostic data of febrile patients recruited for the largest field study on leptospirosis in Sri Lanka. Data In Brief 2022, 43: 108378. PMID: 35770027, PMCID: PMC9234077, DOI: 10.1016/j.dib.2022.108378.Peer-Reviewed Original ResearchResponse of Human Toll–Like Receptor 2 During the Infection of Leptospirosis
Kappagoda C, Senavirathna I, Warnasekara J, Srimantha S, De Silva N, Agampodi S. Response of Human Toll–Like Receptor 2 During the Infection of Leptospirosis. International Journal Of Infectious Diseases 2022, 116: s71. DOI: 10.1016/j.ijid.2021.12.167.Peer-Reviewed Original ResearchToll-like receptor2Human TLR2 geneTLR2 geneMouse modelToll-like receptor 2Human Toll-like receptor 2Results 64 patientsInnate immune responsePathogenic Leptospira sppGene expression analysisImmune responseStudy populationReceptor 2Receptor responsesTLR2 regulationPatientsLeptospirosisLeptospira sppNew therapeuticsWhole bloodGene expression studiesGene amplificationReverse transcriptaseInfectionTotal RNANeglecting the neglected during the COVID-19 pandemic: the case of leptospirosis in Sri Lanka
Warnasekara J, Agampodi S. Neglecting the neglected during the COVID-19 pandemic: the case of leptospirosis in Sri Lanka. Epidemiology And Health 2022, 44: e2022015. PMID: 35038829, PMCID: PMC9117097, DOI: 10.4178/epih.e2022015.Peer-Reviewed Original ResearchConceptsCOVID-19 pandemicCoronavirus disease 2019 (COVID-19) pandemicPublic health surveillance systemsCases of leptospirosisControl of leptospirosisDisease 2019 pandemicHealth surveillance systemsGlobal health systemLarge outbreakInfectious diseasesHealth systemLeptospirosisTransmission dynamicsSurveillance systemPandemicSurveillanceSri LankaDisease
2021
Expression of human toll-like receptor-2 in leptospirosis and improving the validity of gene expression analysis
Kappagoda C, Senavirathna R, Warnasekara Y, Srimantha L, De Silva L, Agampodi S. Expression of human toll-like receptor-2 in leptospirosis and improving the validity of gene expression analysis. Sri Lankan Journal Of Infectious Diseases 2021, 11: 3. DOI: 10.4038/sljid.v11i0.8456.Peer-Reviewed Original Research
2020
Complete Genome Sequence of Leptospira interrogans Strains FMAS_KW1, FMAS_KW2 and FMAS_AW1 Isolated from Leptospirosis Patients from Karawanalla and Awissawella, Sri Lanka
Senevirathna I, Jayasundara D, Lefler JP, Chaiboonm KL, Warnasekara J, Agampodi S, Matthias MA, Vinetz JM. Complete Genome Sequence of Leptospira interrogans Strains FMAS_KW1, FMAS_KW2 and FMAS_AW1 Isolated from Leptospirosis Patients from Karawanalla and Awissawella, Sri Lanka. Journal Of Genomics 2020, 8: 49-52. PMID: 32494307, PMCID: PMC7256012, DOI: 10.7150/jgen.43953.Peer-Reviewed Original Research
2019
Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study
Agampodi S, Warnasekara J, Jayasundara D, Senawirathna I, Gamage C, Kularatne S, Siribaddana S, Maththias M, Vinetz J. Study protocol: characterising the clinical, epidemiological and aetiological aspects of leptospirosis in Sri Lanka: a hospital based clinico-epidemiological study. BMJ Open 2019, 9: e027850. PMID: 31511281, PMCID: PMC6738675, DOI: 10.1136/bmjopen-2018-027850.Peer-Reviewed Original ResearchConceptsEthics Review CommitteeMultilocus sequence typingOutpatient departmentClinico-epidemiological studyMulticentre prospective studyDay of admissionMicroscopic agglutination testPresence of diseaseHospital stayFebrile patientsMedical wardsProspective studySri Lankan isolatesRisk factorsVenous bloodDisease progressionEthical clearanceHigh incidenceAetiological aspectsPatientsAgglutination testMolecular epidemiologyPatient contextBedside inoculationLeptospirosisA Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka
Schønning MH, Phelps MD, Warnasekara J, Agampodi SB, Furu P. A Case–Control Study of Environmental and Occupational Risks of Leptospirosis in Sri Lanka. EcoHealth 2019, 16: 534-543. PMID: 31664587, DOI: 10.1007/s10393-019-01448-w.Peer-Reviewed Original ResearchConceptsPaddy workersOdds ratioSignificant higher riskCase-control studyHigher odds ratioPublic health interventionsTransmission of leptospirosisRisk factorsOccupational exposureProtective effectHigh riskHigh incidenceRat exposureHuman leptospirosisViral infectionHealth interventionsZoonotic infectionOccupational riskLeptospirosisLeptospirosis transmissionStudy sampleOccupational groupsInfectionStructured interviewsHousehold animals
2017
Leptospirosis in Sri Lanka
Warnasekara J, Agampodi S. Leptospirosis in Sri Lanka. Sri Lankan Journal Of Infectious Diseases 2017, 7: 67-75. DOI: 10.4038/sljid.v7i2.8155.Peer-Reviewed Original ResearchCase fatality ratioImportant infectious diseasesRisk groupsFatality ratioDifferent occupational categoriesLarge outbreakInfectious diseasesHuman casesLeptospirosisDiseaseFurther studiesComplicated casesExposure riskOccupational categoriesPriority research areasDifferent strainsSri LankaOutbreakClinicopathologyCasesYearsPhysiciansDiagnosis
2016
Situation of Sri Lanka, where autochthonous malaria is no longer a problem, and other infections dominate, such as dengue, leptospirosis and rickettsioses
Agampodi S, Wijerathne B, Weerakoon K. Situation of Sri Lanka, where autochthonous malaria is no longer a problem, and other infections dominate, such as dengue, leptospirosis and rickettsioses. Current Opinion In Infectious Diseases 2016, 29: 446-452. PMID: 27479026, DOI: 10.1097/qco.0000000000000303.Peer-Reviewed Original ResearchConceptsAutochthonous malariaCase fatality rateCommunicable disease controlReduction of malariaCommunicable disease control programmesControl programsClinical managementMalaria casesDisease control programsFatality rateCommunicable diseasesSuccessful control programsMalaria controlDisease controlInfectious diseasesMalariaDiseaseTropical diseasesDiagnostic facilitiesVector control activitiesLeptospirosisDengueTime trendsVector-borne diseasesRickettsiosesRedefining Gold Standard Testing for Diagnosing Leptospirosis: Further Evidence from a Well-Characterized, Flood-Related Outbreak in Sri Lanka.
Agampodi SB, Dahanayaka NJ, Nöckler K, Mayer-Scholl A, Vinetz JM. Redefining Gold Standard Testing for Diagnosing Leptospirosis: Further Evidence from a Well-Characterized, Flood-Related Outbreak in Sri Lanka. American Journal Of Tropical Medicine And Hygiene 2016, 95: 531-536. PMID: 27402521, PMCID: PMC5014254, DOI: 10.4269/ajtmh.16-0033.Peer-Reviewed Original ResearchConceptsEnzyme-linked immunosorbent assayMicroscopic agglutination testIgG enzyme-linked immunosorbent assayQuantitative polymerase chain reactionImmunoglobulin G (IgG) ELISAHouse enzyme-linked immunosorbent assayDays of illnessAcute-phase seraCases of leptospirosisAvailable enzyme-linked immunosorbent assayGold standard testingMAT positive samplesDiagnostic test resultsAcute specimensAcute phasePatient populationPhase seraPolymerase chain reactionBlood samplesSerological sensitivityAgglutination testImmunosorbent assayLeptospirosisLeptospira kirschneriDominant serovarsEtiological agents causing leptospirosis in Sri Lanka: A review
Naotunna C, Agampodi SB, Agampodi TC. Etiological agents causing leptospirosis in Sri Lanka: A review. Asian Pacific Journal Of Tropical Medicine 2016, 9: 390-394. PMID: 27086159, DOI: 10.1016/j.apjtm.2016.03.009.Peer-Reviewed Original ResearchEtiological agentDirect patient samplesPatients/animalsArticles/reportsMulti-locus sequence typingIsolation of LeptospiraSri Lankan isolatesHuman leptospirosisLocus sequence typingPatient samplesLeptospira santarosaiLeptospira kirschneriLeptospira interrogansLeptospira borgpeterseniiEpidemiology papersSequence typingLeptospirosisLeptospiraNew typing methodTyping methodsRecent studiesIsolatesSri LankaReviewAgents
2015
Outbreak Investigation of Leptospirosis in Padaviya, Sri Lanka
Ramsey A, Rubin-Smith J, Norwich A, Katumuluwa S, Hettiarachchi A, Wimalage S, Danushka W, Madushanka T, Nadeeshani A, Thilakarathna C, Sewwandi L, Malhari T, Sirisena P, Agampodi S. Outbreak Investigation of Leptospirosis in Padaviya, Sri Lanka. Anuradhapura Medical Journal 2015, 9: 23-26. DOI: 10.4038/amj.v9i2.7532.Peer-Reviewed Original ResearchNumber of casesCases of leptospirosisDiagnosis of leptospirosisRegional Health AuthorityRenal compromiseCardiovascular symptomsClinical presentationRetrospective reviewResultsA totalSame hospitalHigh prevalenceBase hospitalHospital staffPatientsHealth authoritiesOutbreak investigationLeptospirosisTropical diseasesPrevious outbreaksHypotensionBradycardiaRecent outbreakAdmissionHospitalParticular outbreak
2014
Validity of a commercially available IgM ELISA test for diagnosing acute leptospirosis in high endemic districts of Sri Lanka
Agampodi S, Thevanesam V, Senaratne T. Validity of a commercially available IgM ELISA test for diagnosing acute leptospirosis in high endemic districts of Sri Lanka. Sri Lankan Journal Of Infectious Diseases 2014, 4: 83. DOI: 10.4038/sljid.v4i2.6952.Peer-Reviewed Original ResearchIgM ELISADiagnosis of leptospirosisConvalescent samplesImmunoglobulin M enzymeDays of illnessComparison groupCases of leptospirosisIgM ELISA kitResultsThe study sampleDepartment of MicrobiologyCare diagnostic testsFebrile patientsAcute leptospirosisFever patientsAcute samplesFaculty of MedicineHuman leptospirosisDay 14Disease confirmationELISA kitLeptospirosisPatientsDiagnostic testsImmunosorbent assayStudy sampleCharacteristics of Rural Leptospirosis Patients Admitted to Referral Hospitals During the 2008 Leptospirosis Outbreak in Sri Lanka: Implications for Developing Public Health Control Measures
Agampodi SB, Nugegoda DB, Thevanesam V, Vinetz JM. Characteristics of Rural Leptospirosis Patients Admitted to Referral Hospitals During the 2008 Leptospirosis Outbreak in Sri Lanka: Implications for Developing Public Health Control Measures. American Journal Of Tropical Medicine And Hygiene 2014, 92: 139-144. PMID: 25331809, PMCID: PMC4347370, DOI: 10.4269/ajtmh.14-0465.Peer-Reviewed Original ResearchConceptsRisk factorsHospital-based case-control studyPublic health control measuresCase-control studyExposure risk factorsHealth control measuresFebrile patientsReferral hospitalCase patientsLeptospirosis patientsMean ageControl studyTropical settingsPatientsVicinity of homeLeptospirosis outbreaksDog ownershipIndividual exposureLeptospirosisMain predictorsDisease transmissionControl measuresMajor roleHospitalSettingHantavirus infection mimicking leptospirosis: how long are we going to rely on clinical suspicion?
Dahanayaka NJ, Agampodi SB, Bandaranayaka AK, Priyankara S, Vinetz JM. Hantavirus infection mimicking leptospirosis: how long are we going to rely on clinical suspicion? The Journal Of Infection In Developing Countries 2014, 8: 1072-5. PMID: 25116678, DOI: 10.3855/jidc.4115.Peer-Reviewed Original ResearchConceptsHantavirus infectionPopulation-based prospective studyDiagnostic facilitiesAcute hantavirus infectionClassical clinical pictureCases of leptospirosisClinical suspicionClinical pictureProspective studyEpidemiological featuresRetrospective analysisInfectious diseasesInfectionSerum samplesMedical staffLeptospirosisLeptospirosis outbreaksFeverDiseaseDiagnosisSuspicionSri LankaGlobalization of leptospirosis through travel and migration
Bandara M, Ananda M, Wickramage K, Berger E, Agampodi S. Globalization of leptospirosis through travel and migration. Globalization And Health 2014, 10: 61. PMID: 25112368, PMCID: PMC4131158, DOI: 10.1186/s12992-014-0061-0.Peer-Reviewed Original ResearchConceptsMajor independent risk factorIndependent risk factorEpidemiology of leptospirosisWidespread zoonotic diseaseRisk factorsContribution of travelOccupational exposurePreventive strategiesHigh-income countriesEndemic countriesDisease acquisitionHealth professionalsTotal caseloadHealth systemLeptospirosisDiagnostic facilitiesDiseaseZoonotic diseaseInternational travelGlobal travelPrevious studiesLongitudinal dataClear increaseEpidemiologyDiagnosisRegional Differences of Leptospirosis in Sri Lanka: Observations from a Flood-Associated Outbreak in 2011
Agampodi SB, Dahanayaka NJ, Bandaranayaka AK, Perera M, Priyankara S, Weerawansa P, Matthias MA, Vinetz JM. Regional Differences of Leptospirosis in Sri Lanka: Observations from a Flood-Associated Outbreak in 2011. PLOS Neglected Tropical Diseases 2014, 8: e2626. PMID: 24454971, PMCID: PMC3894175, DOI: 10.1371/journal.pntd.0002626.Peer-Reviewed Original ResearchConceptsOutbreak of leptospirosisClinical presentationAcute renal failureDifferent clinical presentationsCross-sectional studyMedian bacterial loadPublic health interventionsPublic health relevanceLeptospira interrogans serovar laiRenal failureSevere thrombocytopeniaInfectious disease epidemicsDifferent Leptospira speciesEtiological causesImportant causeHealth interventionsHealth relevanceLeptospirosisTropical diseasesBacterial loadLeptospira kirschneriLeptospirosis outbreaksSerovar laiLeptospira speciesL. kirschneri
2013
Outbreak of leptospirosis after white-water rafting: sign of a shift from rural to recreational leptospirosis in Sri Lanka?
AGAMPODI SB, KARUNARATHNA D, JAYATHILALA N, RATHNAYAKA H, AGAMPODI TC, KARUNANAYAKA L. Outbreak of leptospirosis after white-water rafting: sign of a shift from rural to recreational leptospirosis in Sri Lanka? Epidemiology And Infection 2013, 142: 843-846. PMID: 23800619, PMCID: PMC9151153, DOI: 10.1017/s0950268813001465.Peer-Reviewed Original ResearchConceptsMAT titresPositive IgM ELISAClassical clinical featuresAnti-leptospiral antibodiesHistory of leptospirosisOutbreak of leptospirosisEpidemiology of leptospirosisAfebrile patientsClinical featuresAcute leptospirosisFever patientsIgM ELISAAffluent urban populationsOccupational hazardsOutbreak investigationLeptospirosisLeptospirosis outbreaksOffice workersPatientsTitresWeeksExposureParticipantsOutbreakRecreational activities
2012
Utility and Limitations of Direct Multi-Locus Sequence Typing on qPCR-Positive Blood to Determine Infecting Leptospira Strain
Agampodi SB, Moreno AC, Vinetz JM, Matthias MA. Utility and Limitations of Direct Multi-Locus Sequence Typing on qPCR-Positive Blood to Determine Infecting Leptospira Strain. American Journal Of Tropical Medicine And Hygiene 2012, 88: 184-185. PMID: 23208890, PMCID: PMC3541733, DOI: 10.4269/ajtmh.2012.12-0526.Peer-Reviewed Original ResearchConceptsPolymerase chain reaction-positive samplesBacterial loadLeptospira strainsMLST profilesAcute illnessBlood samplesHuman leptospirosisComplete MLST profilesCulture isolationMulti-locus sequenceHuman bloodSequence typesBloodCentral Sri LankaInterpretable dataMolecular characterizationIllnessLeptospirosisST44