2020
Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients
Landry ML, Criscuolo J, Peaper DR. Challenges in use of saliva for detection of SARS CoV-2 RNA in symptomatic outpatients. Journal Of Clinical Virology 2020, 130: 104567. PMID: 32750665, PMCID: PMC7392849, DOI: 10.1016/j.jcv.2020.104567.Peer-Reviewed Original ResearchMeSH KeywordsBetacoronavirusClinical Laboratory TechniquesCoronavirus InfectionsCOVID-19COVID-19 TestingCOVID-19 VaccinesHumansMolecular Diagnostic TechniquesNasopharynxOutpatientsPandemicsPneumonia, ViralProspective StudiesReal-Time Polymerase Chain ReactionRNA, ViralSalivaSARS-CoV-2Specimen HandlingConceptsNasopharyngeal swabsReal-time RT-PCRSymptomatic outpatientsRT-PCRSARS-CoV-2 positivitySaliva samplesSARS-CoV-2 testingMedian cycle threshold valuePure salivaSARS-CoV-2 RNA detectionSARS-CoV-2 RNACoV-2 testingUse of salivaCycle threshold valuesSymptomatic patientsOverall sensitivityRNA detectionSalivaCOVID-19OutpatientsHigh-volume testingPatientsSwabs
2008
Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients
Landry ML, Cohen S, Ferguson D. Real-time PCR compared to Binax NOW and cytospin-immunofluorescence for detection of influenza in hospitalized patients. Journal Of Clinical Virology 2008, 43: 148-151. PMID: 18639488, PMCID: PMC7173065, DOI: 10.1016/j.jcv.2008.06.006.Peer-Reviewed Original ResearchMeSH KeywordsAdolescentAdultAgedAged, 80 and overAntibodies, MonoclonalChildChild, PreschoolFluorescent Antibody Technique, DirectHospitalizationHumansImmunoassayInfantInfluenza A virusInfluenza B virusInfluenza, HumanMiddle AgedNasopharynxReagent Kits, DiagnosticReproducibility of ResultsReverse Transcriptase Polymerase Chain ReactionSensitivity and SpecificityTaq PolymeraseTime FactorsVirus CultivationYoung AdultConceptsHospitalized patientsTaqMan RT-PCRRT-PCRInfluenza ARapid flu testsDiagnosis of influenzaInfluenza-positive samplesDetection of influenzaReal-time RT-PCRInfluenza BInfluenza diagnosisPatient managementReal-time PCRMore accurate testsPatientsBinaxTrue positive samplesRapid testClinical samplesPositive samplesFurther studiesHospitalAccurate testInfluenzaDiagnosis
2003
Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens
Landry ML, Garner R, Ferguson D. Comparison of the NucliSens Basic Kit (Nucleic Acid Sequence-Based Amplification) and the Argene Biosoft Enterovirus Consensus Reverse Transcription-PCR Assays for Rapid Detection of Enterovirus RNA in Clinical Specimens. Journal Of Clinical Microbiology 2003, 41: 5006-5010. PMID: 14605131, PMCID: PMC262477, DOI: 10.1128/jcm.41.11.5006-5010.2003.Peer-Reviewed Original ResearchConceptsNucleic acid sequence-based amplificationNucliSens Basic KitRT-PCREnterovirus RNABasic KitEnterovirus-positive samplesReverse transcription-PCR assayTranscription-PCR assayNasopharyngeal samplesRT-PCR kitVirus isolationClinical specimensTranscription-PCRPositive samplesComparable sensitivityKitMolecular methodsEnterovirusesComparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus
Habib-Bein NF, Beckwith WH, Mayo D, Landry ML. Comparison of SmartCycler Real-Time Reverse Transcription-PCR Assay in a Public Health Laboratory with Direct Immunofluorescence and Cell Culture Assays in a Medical Center for Detection of Influenza A Virus. Journal Of Clinical Microbiology 2003, 41: 3597-3601. PMID: 12904361, PMCID: PMC179819, DOI: 10.1128/jcm.41.8.3597-3601.2003.Peer-Reviewed Original ResearchConceptsVirus-positive specimensDirect immunofluorescence antibody stainingPublic health laboratoriesHealth laboratoriesRT-PCRMedical CenterRapid diagnosisYale-New Haven HospitalReal-time RT-PCRResults of cultureRhesus monkey kidney cell culturesImmunofluorescence antibody stainingReal-time reverse transcriptionInfluenza A virusesMonkey kidney cell culturesConventional RT-PCR analysisReal-time RT-PCR systemRespiratory specimensInfluenza APatient managementDirect immunofluorescenceCell culturesKidney cell culturesRapid turnaround timeA virus