2021
Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection
Oliva Chávez AS, Wang X, Marnin L, Archer NK, Hammond HL, Carroll EEM, Shaw DK, Tully BG, Buskirk AD, Ford SL, Butler LR, Shahi P, Morozova K, Clement CC, Lawres L, Neal A, Mamoun CB, Mason KL, Hobbs BE, Scoles GA, Barry EM, Sonenshine DE, Pal U, Valenzuela JG, Sztein MB, Pasetti MF, Levin ML, Kotsyfakis M, Jay SM, Huntley JF, Miller LS, Santambrogio L, Pedra JHF. Tick extracellular vesicles enable arthropod feeding and promote distinct outcomes of bacterial infection. Nature Communications 2021, 12: 3696. PMID: 34140472, PMCID: PMC8211691, DOI: 10.1038/s41467-021-23900-8.Peer-Reviewed Original ResearchMeSH KeywordsAnaplasma phagocytophilumAnimalsArthropodsBacterial InfectionsCell LineDermacentorExtracellular VesiclesFrancisella tularensisGene OntologyHumansInflammationIntravital MicroscopyIxodesMaleMiceMice, Inbred C57BLMice, KnockoutMicroscopy, Electron, TransmissionProteomicsR-SNARE ProteinsSkinTandem Mass SpectrometryTicksT-LymphocytesVesicle-Associated Membrane Protein 2ConceptsExtracellular vesiclesBiology of arthropodsSynaptobrevin 2Pathogen Francisella tularensisMammalian hostsArthropodsVector feedingDistinct outcomesPathogen transmissionVesiclesMicrobial spreadingVector-borne diseasesFrancisella tularensisBacterial infectionsTicks DermacentorIxodes scapularisAnaplasma phagocytophilumBiologySkin immunitySnareDendritic epidermal T cellsPathogensHostT cellsTularensis
2019
Evidence for vesicle-mediated antigen export by the human pathogen Babesia microti
Thekkiniath J, Kilian N, Lawres L, Gewirtz MA, Graham MM, Liu X, Ledizet M, Mamoun C. Evidence for vesicle-mediated antigen export by the human pathogen Babesia microti. Life Science Alliance 2019, 2: e201900382. PMID: 31196872, PMCID: PMC6572159, DOI: 10.26508/lsa.201900382.Peer-Reviewed Original ResearchConceptsApicomplexan parasitesCell fractionation studiesImmunoelectron microscopy analysisMode of secretionInvasion of erythrocytesParasite effectorsTrafficking motifsPlasma membraneExport mechanismClose relativesParasitophorous vacuoleHost erythrocyteMorphogenic changesFractionation studiesNovel mechanismHuman malariaFatal tick-borne diseaseMalaria-like illnessMouse red blood cellsParasitesAntigen exportTick-borne diseaseRed blood cellsHuman babesiosisImmunodominant antigens