The Flavell laboratory operates a facility that routinely generates transgenic mice by microinjection of blastocysts, and knockout mice by homologous recombination in embryonic stem cells followed by microinjection of the cells.
Facilities
Fluorescence in situ and in vivo microscopy
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The Department has available multiple fluorescence and confocal microscopes suitable for the detection of up to 6 channels ideal for in situ microscopy of cells and tissues that are shared by individual laboratories. The department has access to an Olympus BX61WI fluorescence microscope equipped with a 20×, 0.95NA water immersion Olympus objective, single-beam LaVision TriM laser-scanning microscope (LaVision Biotec) run with Inspector software, and illuminated with a Chameleon Vision II Ti:Sapphire laser (Coherent) suitable for intravital 2-photon microscopy. The intravital imaging facility is run by David Gonzalez and directed by Dr. Ann Haberman, in the Departemnt of Laboratory Medicine.
Other
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Several excellent core facilities in other departments at Yale are available including peptide synthesis, oligonucleotide synthesis, protein sequencing, and DNA sequencing.
Transgenic and Knock Out Mice Yale Flow Cytometry Facility Comprehensive flow cytometric analysis and sorting including instrumentation, technical support, training and consultation.Read MoreYale Genome Editing Center Services and Resources for Creating Genetically Engineered MiceRead MoreOther Yale Core Facilities Services including peptide synthesis, oligonucleotide synthesis, protein sequencing, and DNA sequencing.Read More
Cell Imaging
An example of Cell Imaging gathered utilizing the onsite facilities.