BD LSR Fortessa
Location: 300 George Street Lab 2331 & Amistad Lab 105
Contact: Lesley.Devine@yale.edu
The Fortessas occupy rooms that are specifically designated as BSL2 space for the purpose of sorting and analyzing unfixed human samples obtained from healthy individuals. While gloves are enough personal protective equipment (PPE) when handling non-human samples, it is important to remember that the analysis of unfixed human samples necessitates additional PPE (such as a face shield, to be provided by the user) and specific steps for equipment disinfection that are not required for other cytometers. It is crucial to prioritize personal protection in these rooms. Moreover, human samples from individuals known to be infected with HIV or HepB/C must be fixed before they can be analyzed on any cytometer, including the Fortessas.
UV Laser - 355nm 60 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 740/35 | 690LP | BUV737 |
| B | 450/50 | 410LP | DAPI, AlexaFluor 350 |
| C | 379/28 | - | BUV395 |
Violet Laser - 405nm 100 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 710/50 | 690LP | BV711 |
| B | 640/40 | 635LP | Qdot 655, BV650 |
| C | 525/50 | 505LP | AmCyan, V500, BV510 |
| D | 450/50 | - | BV421, Pac-Blue, BV450 |
Please notify FACS Facility Staff if you need to use BV650 on Fortessa Amistad and filter will be adjusted. It is current set to detect BV605.
Blue Laser - 488nm 50 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 685/35 | 670LP | PerCP, PerCP-Cy5.5 |
| B | 515/20 | 505LP | FITC, GFP, CFSE |
| C | 488/10 | SSC |
Green Laser - 532nm 150 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/40 | 735LP | PE-Cy7 |
| B | 710/50 | 685LP | PE-Cy5.5 |
| C | 660/20 | 635LP | PE-Cy5 |
| D | 610/20 | 600LP | PE-Texas Red, PI
|
| E | 582/15 | PE |
Red Laser - 640 nm 100 mW
| PMT | Bandpass | Long Pass | Fluorochrome |
| A | 780/60 | 750LP | APC-Cy7, APC-H7, APC-Alexa Fluor 750 |
| B | 730/45 | 685LP | Alexa Fluor 680, Alexa Fluor 700 |
| C | 670/30 | APC, Alexa Fluor 647 |