2024
Akt is a mediator of artery specification during zebrafish development
Zhou W, Ghersi J, Ristori E, Semanchik N, Prendergast A, Zhang R, Carneiro P, Baldissera G, Sessa W, Nicoli S. Akt is a mediator of artery specification during zebrafish development. Development 2024, 151: dev202727. PMID: 39101673, PMCID: PMC11441982, DOI: 10.1242/dev.202727.Peer-Reviewed Original ResearchArterial specificationEndothelial cellsVascular endothelial growth factor ADorsal aortaEndothelial growth factor ASingle-cell RNA sequencing analysisGrowth factor AArtery endothelial cellsEmbryonic cardiovascular systemConstitutively active Akt1Ligand-independent activationActivation of NotchArteriovenous malformationsCongenital malformationsRNA sequencing analysisVEGF-AProtein kinase BUpstream of NotchSequence analysisCardiovascular developmentSpecific expressionAkt kinaseActive Akt1Zebrafish developmentCardiovascular systemHemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis
Cheng S, Xia I, Wanner R, Abello J, Stratman A, Nicoli S. Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis. ELife 2024, 13 DOI: 10.7554/elife.94094.3.Peer-Reviewed Original ResearchVascular smooth muscle cellsVascular smooth muscle cell differentiationWall shear stressVSMC differentiationEndothelial cellsAnalysis of blood flowBlood flowShear stressBrain arteriesPulsatile flowCerebrovascular diseaseDedifferentiated vascular smooth muscle cellsRegulate cerebral blood flowSmooth muscle cellsRed blood cell velocityDedifferentiation of vascular smooth muscle cellsCerebral blood flowBlood cell velocityArterial muscularizationVenous plexusCell progenitorsMuscle cellsBlood flow activationArteryFlowHemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis
Cheng S, Xia I, Wanner R, Abello J, Stratman A, Nicoli S. Hemodynamics regulate spatiotemporal artery muscularization in the developing circle of Willis. ELife 2024, 13: rp94094. PMID: 38985140, PMCID: PMC11236418, DOI: 10.7554/elife.94094.Peer-Reviewed Original ResearchConceptsVascular smooth muscle cellsWall shear stressVascular smooth muscle cell differentiationVSMC differentiationEndothelial cellsAnalysis of blood flowBlood flowShear stressBrain arteriesPulsatile flowCerebrovascular diseaseDedifferentiated vascular smooth muscle cellsRegulate cerebral blood flowSmooth muscle cellsRed blood cell velocityDedifferentiation of vascular smooth muscle cellsCerebral blood flowBlood cell velocityArterial muscularizationVenous plexusCell progenitorsMuscle cellsBlood flow activationArteryFlowLatrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions
Tanaka K, Chen M, Prendergast A, Zhuang Z, Nasiri A, Joshi D, Hintzen J, Chung M, Kumar A, Mani A, Koleske A, Crawford J, Nicoli S, Schwartz M. Latrophilin-2 mediates fluid shear stress mechanotransduction at endothelial junctions. The EMBO Journal 2024, 43: 3175-3191. PMID: 38886581, PMCID: PMC11294477, DOI: 10.1038/s44318-024-00142-0.Peer-Reviewed Original ResearchLatrophilin-2Affinity purification methodCell-cell junctionsHuman genetic dataPECAM-1SiRNA screenGenetic dataEndothelial cell response to fluid shear stressGA proteinsDownstream eventsEndothelial-specific knockoutG-proteinActivity assayShear stress mechanotransductionPlexin-D1Endothelial signalingJunctional complexesPurification methodVE-cadherinResponse to fluid shear stressVascular developmentGA residuesEndothelial junctionsGPCRsVEGF receptors
2023
Mutation of key signaling regulators of cerebrovascular development in vein of Galen malformations
Zhao S, Mekbib K, van der Ent M, Allington G, Prendergast A, Chau J, Smith H, Shohfi J, Ocken J, Duran D, Furey C, Hao L, Duy P, Reeves B, Zhang J, Nelson-Williams C, Chen D, Li B, Nottoli T, Bai S, Rolle M, Zeng X, Dong W, Fu P, Wang Y, Mane S, Piwowarczyk P, Fehnel K, See A, Iskandar B, Aagaard-Kienitz B, Moyer Q, Dennis E, Kiziltug E, Kundishora A, DeSpenza T, Greenberg A, Kidanemariam S, Hale A, Johnston J, Jackson E, Storm P, Lang S, Butler W, Carter B, Chapman P, Stapleton C, Patel A, Rodesch G, Smajda S, Berenstein A, Barak T, Erson-Omay E, Zhao H, Moreno-De-Luca A, Proctor M, Smith E, Orbach D, Alper S, Nicoli S, Boggon T, Lifton R, Gunel M, King P, Jin S, Kahle K. Mutation of key signaling regulators of cerebrovascular development in vein of Galen malformations. Nature Communications 2023, 14: 7452. PMID: 37978175, PMCID: PMC10656524, DOI: 10.1038/s41467-023-43062-z.Peer-Reviewed Original ResearchConceptsEphrin receptor B4Galen malformationBrain arteriovenous malformationsP120 RasGAPTransmitted variantsArteriovenous malformationsDe novo variantsSingle-cell transcriptomesSignificant burdenCerebrovascular developmentIntegrative genomic analysisEndothelial cellsVenous networkAdditional probandsMalformationsNovo variantsMissense variantsGenomic analysisDevelopmental angiogenesisVascular developmentDamaging variantsVeinRasGAPIntegrated analysisPatientsDistinct hypoxia-induced translational profiles of embryonic and adult-derived macrophages
Wilcox N, Yarovinsky T, Pandya P, Ramgolam V, Moro A, Wu Y, Nicoli S, Hirschi K, Bender J. Distinct hypoxia-induced translational profiles of embryonic and adult-derived macrophages. IScience 2023, 26: 107985. PMID: 38047075, PMCID: PMC10690575, DOI: 10.1016/j.isci.2023.107985.Peer-Reviewed Original ResearchBone marrow-derived macrophagesTranslation rateAffinity purification assaysGene expression programsTissue-resident macrophagesExpression programsTranscriptional signalsTranslational profilesTranscriptome analysisPosttranscriptional regulationMRNA translationRNA-seqMarrow-derived macrophagesPurification assaysRNA expression levelsProtein HuR.MRNA stabilityDistinct hypoxiaEmbryonic originPotential therapeutic targetTranscriptsExpression levelsAcute perturbationTherapeutic targetIschemic myocardiumHaematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium
Ghersi J, Baldissera G, Hintzen J, Luff S, Cheng S, Xia I, Sturgeon C, Nicoli S. Haematopoietic stem and progenitor cell heterogeneity is inherited from the embryonic endothelium. Nature Cell Biology 2023, 25: 1135-1145. PMID: 37460694, PMCID: PMC10415179, DOI: 10.1038/s41556-023-01187-9.Peer-Reviewed Original ResearchConceptsHaemogenic endothelial cellsHaematopoietic stemPost-transcriptional repressionHuman pluripotent stem cellsProgenitor cell heterogeneityCell cycle statePluripotent stem cellsG2/MZebrafish leadsAorta-gonadBlood lineagesEmbryonic endotheliumExpansion of HSPCsEndothelial cellsProliferative outputDifferent cell cycle statesLymphoid progenitorsCell heterogeneityLineagesMiR-128Jag1bHSPCsMyeloid lineageStem cellsCycle state3067 – HEMATOPOIETIC STEM AND PROGENITOR CELL HETEROGENEITY IS INHERITED FROM THE EMBRYONIC ENDOTHELIUM.
Ghersi J, Baldissera G, Hintzen J, Luff S, Cheng S, Xia I, Sturgeon C, Nicoli S. 3067 – HEMATOPOIETIC STEM AND PROGENITOR CELL HETEROGENEITY IS INHERITED FROM THE EMBRYONIC ENDOTHELIUM. Experimental Hematology 2023, 124: s83. DOI: 10.1016/j.exphem.2023.06.174.Peer-Reviewed Original ResearchHematopoietic stem/progenitor cellsMiR-128Hemogenic endothelial cellsMature blood cellsHuman pluripotent stem cellsCell cycle stateStem/progenitor cellsPluripotent stem cellsG2/MHematopoietic transitionEmbryonic endotheliumProliferative outputDifferent cell cycle statesAdult stageLymphoid progenitorsCell heterogeneityLineagesJag1bMyeloid lineageStem cellsProgenitor cellsCycle stateBlood progenitorsHemECsWnt
2022
Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells
Kshitiz, Afzal J, Suhail Y, Chang H, Hubbi M, Hamidzadeh A, Goyal R, Liu Y, Sun P, Nicoli S, Dang C, Levchenko A. Lactate-dependent chaperone-mediated autophagy induces oscillatory HIF-1α activity promoting proliferation of hypoxic cells. Cell Systems 2022, 13: 1048-1064.e7. PMID: 36462504, PMCID: PMC10012408, DOI: 10.1016/j.cels.2022.11.003.Peer-Reviewed Original ResearchConceptsHIF-1α activityActivation of genesChaperone-mediated autophagyCancer cellsCell divisionIndividual cancer cellsRegulated processPatient-derived cancer cellsSubset of cellsMolecular mechanismsFluorescent reportersSingle-cell responsesCancer cell linesCell linesGenesHypoxic tumor cellsHIF-1αHypoxic conditionsCellsBroad suppressionAggressive growthTumor cellsHypoxic cellsOscillatory activityExtracellular lactate
2017
Chapter 15 Comparative Functions of miRNAs in Embryonic Neurogenesis and Neuronal Network Formation
Ristori E, Nicoli S. Chapter 15 Comparative Functions of miRNAs in Embryonic Neurogenesis and Neuronal Network Formation. 2017, 265-282. DOI: 10.1016/b978-0-12-804402-5.00015-7.Peer-Reviewed Original ResearchTarget mRNA degradationCell fate determinationGene regulatory pathwaysDynamic spatiotemporal expressionImportance of miRNAsSmall noncoding RNAsStem cell proliferationMulticellular organismsFate determinationMost miRNAsNeuronal network formationTranslational repressionModel organismsNeural stem cell proliferationMRNA degradationPosttranscriptional regulatorsNoncoding RNAsRegulatory pathwaysDevelopmental processesEmbryonic neurogenesisGene expressionSpatiotemporal expressionNovel roleNeuronal differentiationMiRNAs
2016
Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres
Lopez-Ramirez M, Calvo C, Ristori E, Thomas J, Nicoli S. Isolation and Culture of Adult Zebrafish Brain-derived Neurospheres. Journal Of Visualized Experiments 2016 DOI: 10.3791/53617-v.Peer-Reviewed Original Research
2013
A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development
Villefranc JA, Nicoli S, Bentley K, Jeltsch M, Zarkada G, Moore JC, Gerhardt H, Alitalo K, Lawson ND. A truncation allele in vascular endothelial growth factor c reveals distinct modes of signaling during lymphatic and vascular development. Development 2013, 140: 1497-1506. PMID: 23462469, PMCID: PMC3596992, DOI: 10.1242/dev.084152.Peer-Reviewed Original ResearchMeSH KeywordsAllelesAnimalsAnimals, Genetically ModifiedAutocrine CommunicationBlood VesselsCell MovementCodon, NonsenseEmbryo, NonmammalianFemaleLymphatic SystemMiceMice, KnockoutNeovascularization, PhysiologicParacrine CommunicationProtein IsoformsSignal TransductionVascular Endothelial Growth Factor CZebrafishZebrafish ProteinsConceptsMigratory persistenceLymphatic developmentSensitized genetic backgroundTip cell positionVascular endothelial growth factor CEndothelial cell dynamicsDeficient endothelial cellsFactor CTime-lapse analysisMutant embryosVertebrate embryosTruncation alleleEctopic blood vesselsFilopodia stabilityAngiogenesis defectsDistinct modesEndothelial cellsDevelopmental angiogenesisLymphatic vasculatureVascular developmentLymphatic defectsGenetic backgroundReceptor FLT4VEGFCCell position
2012
miRNAs in endothelial cell signaling: The endomiRNAs
Santoro M, Nicoli S. miRNAs in endothelial cell signaling: The endomiRNAs. Experimental Cell Research 2012, 319: 1324-1330. PMID: 23262024, PMCID: PMC3648629, DOI: 10.1016/j.yexcr.2012.12.009.Peer-Reviewed Original ResearchHeparin Derivatives and Semisynthetic Biotechnological Heparins as Angiogenesis Inhibitors
Presta M, Leali D, Stabile H, Ronca R, Camozzi M, Moroni E, Nicoli S, Liekens S, Marco L. Heparin Derivatives and Semisynthetic Biotechnological Heparins as Angiogenesis Inhibitors. 2012, 371-391. DOI: 10.2174/978160805205910502010371.Peer-Reviewed Original Research
2010
Erratum: MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis
Nicoli S, Standley C, Walker P, Hurlstone A, Fogarty K, Lawson N. Erratum: MicroRNA-mediated integration of haemodynamics and Vegf signalling during angiogenesis. Nature 2010, 467: 356-356. DOI: 10.1038/nature09412.Peer-Reviewed Original Research
2008
Fibroblast Growth Factor-2 in Angiogenesis
Presta M, Mitola S, Dell'Era P, Leali D, Nicoli S, Moroni E, Rusnati M. Fibroblast Growth Factor-2 in Angiogenesis. 2008, 77-88. DOI: 10.1007/978-0-387-71518-6_7.Peer-Reviewed Original ResearchFibroblast growth factor-2Growth factor 2Signal transduction pathwaysGrowth factorExtracellular matrix-associated moleculesFactor 2Cell surface receptorsTyrosine kinase receptorsMatrix-associated moleculesHeparan sulfate proteoglycanTransduction pathwaysExtracellular interactionsEndothelial cell surface receptorsKinase receptorsSurface receptorsComplex networksAngiogenic growth factorsMechanism of actionAngiogenic potentialPathological conditionsBiological activityEndothelial cellsAngiogenic activityTumor growthFurther complexity
2005
Heparin Derivatives and Semisynthetic Biotechnological Heparins as Angiogenesis Inhibitors
Presta M, Leali D, Stabile H, Ronca R, Camozzi M, Moroni E, Nicoli S, Liekens S, Rusnati M. Heparin Derivatives and Semisynthetic Biotechnological Heparins as Angiogenesis Inhibitors. 2005, 2: 371-391. DOI: 10.2174/1567204052931087.Peer-Reviewed Original Research