2021
Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore
Li Y, Aksenova V, Tingey M, Yu J, Ma P, Arnaoutov A, Chen S, Dasso M, Yang W. Distinct roles of nuclear basket proteins in directing the passage of mRNA through the nuclear pore. Proceedings Of The National Academy Of Sciences Of The United States Of America 2021, 118: e2015621118. PMID: 34504007, PMCID: PMC8449422, DOI: 10.1073/pnas.2015621118.Peer-Reviewed Original ResearchConceptsNuclear pore complexNuclear basket proteinsNative nuclear pore complexesCopy numberDistinct rolesExport routesSingle-molecule microscopyExport kineticsPore complexNative copyNuclear exportNuclear poresExact copy numberComposite proteinsNup153Live cellsMessenger RNASpecific functionsFull complementProteinSpecific mannerNup50Specific roleExport efficiencyRNA
2020
High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation
Li Y, Tingey M, Ruba A, Yang W. High-speed super-resolution imaging of rotationally symmetric structures using SPEED microscopy and 2D-to-3D transformation. Nature Protocols 2020, 16: 532-560. PMID: 33318694, PMCID: PMC9382854, DOI: 10.1038/s41596-020-00440-x.Peer-Reviewed Original ResearchConceptsSpeed microscopySuper-resolution imaging techniquesDiffraction-limited resolutionComplex optical componentsSuper-resolution imagingSuper-resolution informationOptical componentsSuper-resolution microscopyBiological channelsSpeed imagingRotational symmetryFluorescent moleculesHigh spatiotemporal resolutionMicroscopy approachSymmetric structureImaging techniquesMicroscopySpatiotemporal resolutionEpifluorescence microscopeLive cellsResolutionSubcellular structures