2015
Acyl-CoA Dehydrogenase Drives Heat Adaptation by Sequestering Fatty Acids
K. D, Li Z, Lu AY, Sun F, Chen S, Rothe M, Menzel R, Sun F, Horvitz HR. Acyl-CoA Dehydrogenase Drives Heat Adaptation by Sequestering Fatty Acids. Cell 2015, 161: 1152-1163. PMID: 25981666, PMCID: PMC4441829, DOI: 10.1016/j.cell.2015.04.026.Peer-Reviewed Original ResearchConceptsHigh-resolution crystal structuresMembrane fluidityFatty acidsHeat adaptationNuclear hormone receptorsFat-7Lipid desaturationMolecular basisTemperature shiftFatty acid oxidationDesaturase levelsForms of lifeFundamental processesHormone receptorsAcid oxidationUnprecedented modeExpressionCommon inherited disorderAdaptationEukaryotesCaenorhabditisInherited disorderAcidCrystal structureRegulation
2014
CRISPR-mediated direct mutation of cancer genes in the mouse liver
Xue W, Chen S, Yin H, Tammela T, Papagiannakopoulos T, Joshi NS, Cai W, Yang G, Bronson R, Crowley DG, Zhang F, Anderson DG, Sharp PA, Jacks T. CRISPR-mediated direct mutation of cancer genes in the mouse liver. Nature 2014, 514: 380-384. PMID: 25119044, PMCID: PMC4199937, DOI: 10.1038/nature13589.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBase SequenceBeta CateninCell Transformation, NeoplasticClustered Regularly Interspaced Short Palindromic RepeatsCRISPR-Cas SystemsFemaleGenes, p53Genes, Tumor SuppressorGenetic EngineeringHepatocytesLipid MetabolismLiverLiver NeoplasmsMiceMolecular Sequence DataMutagenesisMutationOncogenesPhosphorylationProto-Oncogene Proteins c-aktPTEN PhosphohydrolaseGenome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells
Wu X, Scott DA, Kriz AJ, Chiu AC, Hsu PD, Dadon DB, Cheng AW, Trevino AE, Konermann S, Chen S, Jaenisch R, Zhang F, Sharp PA. Genome-wide binding of the CRISPR endonuclease Cas9 in mammalian cells. Nature Biotechnology 2014, 32: 670-676. PMID: 24752079, PMCID: PMC4145672, DOI: 10.1038/nbt.2889.Peer-Reviewed Original Research
2012
Frequent Recent Origination of Brain Genes Shaped the Evolution of Foraging Behavior in Drosophila
Chen S, Spletter M, Ni X, White KP, Luo L, Long M. Frequent Recent Origination of Brain Genes Shaped the Evolution of Foraging Behavior in Drosophila. Cell Reports 2012, 1: 118-132. PMID: 22832161, PMCID: PMC4382513, DOI: 10.1016/j.celrep.2011.12.010.Peer-Reviewed Original ResearchMeSH KeywordsAdaptation, PhysiologicalAgingAmino Acid SequenceAnimalsArthropod AntennaeBrainDrosophila melanogasterDrosophila ProteinsEvolution, MolecularFeeding BehaviorFemaleGene Expression ProfilingGene Expression Regulation, DevelopmentalGenes, InsectMaleMolecular Sequence DataMushroom BodiesNeuronsSelection, GeneticSex CharacteristicsSynaptic TransmissionTranscriptomeConceptsNew genesForaging behaviorMushroom bodiesYoung retrogenesAdaptive evolutionPhenotypic evolutionEvolutionary signaturesYoung genesNatural selectionGenetic basisBrain genesExpression profilingComparative behavioral analysisBrain evolutionGenesOlfactory circuitDrosophilaNew functionsAnimal behaviorNeuronal expressionExpressionRetrogenesNeural circuitsEvolutionRecent evolution
2011
Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster
Chen Y, Dai H, Chen S, Zhang L, Long M. Highly Tissue Specific Expression of Sphinx Supports Its Male Courtship Related Role in Drosophila melanogaster. PLOS ONE 2011, 6: e18853. PMID: 21541324, PMCID: PMC3082539, DOI: 10.1371/journal.pone.0018853.Peer-Reviewed Original ResearchMeSH KeywordsAnimal StructuresAnimalsBase PairingBase SequenceBrainConserved SequenceCourtshipDrosophila melanogasterFemaleGene Expression ProfilingGene Expression RegulationGenome, InsectGreen Fluorescent ProteinsMaleMolecular Sequence DataMutationOrgan SpecificityPeripheral Nervous SystemPromoter Regions, GeneticRNA, UntranslatedSexual Behavior, AnimalTransformation, GeneticConceptsDrosophila melanogasterBp regionCourtship behaviorNon-coding RNA genesWhole genome expression profilingBp upstream regionTissue-specific expressionGenome expression profilingMale courtship behaviorMale accessory glandsMelanogaster subgroupDrosophila speciesD. virilisRNA genesGene categoriesProtein functionKnockout mutationsWing hairsMale courtshipTarget genesExpression profilingGFP signalNegative regulatorEnhancer elementsExpression signals
2003
RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong
Chen X, Feng Q, Wu Z, Liu Y, Huang K, Shi R, Chen S, Lu W, Ding M, Collins R, Fung Y, Lau L, Yu A, Chen J. RNA-dependent RNA polymerase gene sequence from foot-and-mouth disease virus in Hong Kong. Biochemical And Biophysical Research Communications 2003, 308: 899-905. PMID: 12927804, DOI: 10.1016/s0006-291x(03)01511-0.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid MotifsAmino Acid SequenceBase SequenceCloning, MolecularConserved SequenceDNA-Directed RNA PolymerasesFoot-and-Mouth Disease VirusGenetic VariationHong KongModels, GeneticMolecular Sequence DataMutationPhylogenyProtein Structure, TertiaryReverse Transcriptase Polymerase Chain ReactionRNA-Dependent RNA PolymeraseRNA, ViralSequence Analysis, DNASequence Homology, Amino AcidConceptsRNA-dependent RNA polymerase gene sequencesNucleotide sequenceRNA polymerase gene sequencesViral RNA-dependent RNA polymeraseRNA-dependent RNA polymeraseAmino acid sequenceAmino acid residuesMouth disease virusPolymerase gene sequencesCommon ancestorEvolutionary treeRNA polymeraseGene sequencesSequence comparisonAcid sequenceSame geneN-terminusDisease virusAcid residuesGenesFunctional relevanceRepresentative isolatesHost immune systemFMDV isolatesHKN/2002