2024
Complex trauma sequelae: Mycobacterium goodii and Priestia endophytica Hardware infection in a patient with Ehlers-Danlos syndrome
Covington A, Cerqueira F, Pavia J, Reynoso D, Ren P. Complex trauma sequelae: Mycobacterium goodii and Priestia endophytica Hardware infection in a patient with Ehlers-Danlos syndrome. BMC Infectious Diseases 2024, 24: 1064. PMID: 39333951, PMCID: PMC11438204, DOI: 10.1186/s12879-024-09970-1.Peer-Reviewed Original ResearchConceptsEhlers-Danlos syndromeSevere left leg painMycobacterium goodiiChronic otitis mediaOpen femur fracturesRecurrent otitis externaDaptomycin treatmentGram-positive bacilliHardware infectionLeg painImmunocompromised patientsPurulent dischargeMotor vehicle collisionsOtitis mediaOrthopedic complicationsOtitis externaHardware fixationFemur fracturesOpportunistic human pathogenEmergency departmentLower extremity amputationPatientsSyndromeHuman pathogensInfection
2023
Function and Cryo-EM structures of broadly potent bispecific antibodies against multiple SARS-CoV-2 Omicron sublineages
Ren P, Hu Y, Peng L, Yang L, Suzuki K, Fang Z, Bai M, Zhou L, Feng Y, Zou Y, Xiong Y, Chen S. Function and Cryo-EM structures of broadly potent bispecific antibodies against multiple SARS-CoV-2 Omicron sublineages. Signal Transduction And Targeted Therapy 2023, 8: 281. PMID: 37518189, PMCID: PMC10387464, DOI: 10.1038/s41392-023-01509-1.Peer-Reviewed Original ResearchRAMIHM generates fully human monoclonal antibodies by rapid mRNA immunization of humanized mice and BCR-seq
Ren P, Peng L, Yang L, Suzuki K, Fang Z, Renauer P, Lin Q, Bai M, Li T, Clark P, Klein D, Chen S. RAMIHM generates fully human monoclonal antibodies by rapid mRNA immunization of humanized mice and BCR-seq. Cell Chemical Biology 2023, 30: 85-96.e6. PMID: 36640761, PMCID: PMC9868106, DOI: 10.1016/j.chembiol.2022.12.005.Peer-Reviewed Original ResearchConceptsHuman monoclonal antibodyHumanized miceMonoclonal antibodiesMemory B cell populationsHumanized transgenic miceBroad antibody responseB cell populationsG protein-coupled receptor targetsNeutralizing antibodiesPeripheral bloodAntibody responseImmunotherapy targetClinical vaccinesPlasma BCell sequencingTransgenic miceImmunization methodReceptor targetsAntibodiesMiceCell populationsHigh potencyImmunizationHigh rateAntibody discovery
2022
LRRC15 inhibits SARS-CoV-2 cellular entry in trans
Song J, Chow RD, Peña-Hernández MA, Zhang L, Loeb SA, So EY, Liang OD, Ren P, Chen S, Wilen CB, Lee S. LRRC15 inhibits SARS-CoV-2 cellular entry in trans. PLOS Biology 2022, 20: e3001805. PMID: 36228039, PMCID: PMC9595563, DOI: 10.1371/journal.pbio.3001805.Peer-Reviewed Original ResearchConceptsExpression of LRRC15Receptor-binding domainViral entryAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionSARS-CoV-2 cellular entrySyndrome coronavirus 2 infectionSARS-CoV-2 entrySpike-mediated entryCoronavirus 2 infectionCOVID-19 patientsCellular entry factorsSARS-CoV-2Attachment factorsACE2-negative cellsEnzyme 2Receptor angiotensinEntry factorsProtective roleLRRC15Spike proteinSame cell typeCRISPR activation screensACE2Cellular entryDevelopment of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2
Ssenyange G, Kerfoot M, Zhao M, Farhadian S, Chen S, Peng L, Ren P, Dela Cruz CS, Gupta S, Sutton RE. Development of an efficient reproducible cell-cell transmission assay for rapid quantification of SARS-CoV-2 spike interaction with hACE2. Cell Reports Methods 2022, 2: 100252. PMID: 35757815, PMCID: PMC9213030, DOI: 10.1016/j.crmeth.2022.100252.Peer-Reviewed Original ResearchConceptsAnti-spike monoclonal antibodiesTransmission assaysTherapeutic antiviral drugsSARS-CoV-2Quantitative readoutVirus-cell bindingRapid quantificationConvalescent seraNeutralization assaysAntiviral drugsResearch reagentsSmall molecule drugsClinical settingViral replicationPseudotyped particlesMonoclonal antibodiesLaboratory equipmentQuantitative assayCAR‐T Therapy in Clinical Practice: Technical Advances and Current Challenges
Ren P, Zhang C, Li W, Wang X, Liang A, Yang G, Xu H, Ma P. CAR‐T Therapy in Clinical Practice: Technical Advances and Current Challenges. Advanced Biology 2022, 6: e2101262. PMID: 35652169, DOI: 10.1002/adbi.202101262.Peer-Reviewed Original ResearchConceptsChimeric antigen receptorCAR T cellsCAR-T therapyClinical trialsCAR T-cell therapyAcute lymphoblastic leukemiaSuccessful clinical outcomeB-cell lymphomaT cell engineeringStandard tumor therapiesAntigen recognition domainComplete responseClinical outcomesTumor toxicityLymphoblastic leukemiaT cellsCell lymphomaClinical experiencePoor efficacyClinical practiceSolid tumorsTumor resistanceTherapyAntitumor efficacyCell therapy
2015
Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome
Gu Urban GJ, Friedman M, Ren P, Törn C, Fex M, Hampe CS, Lernmark Å, Landegren U, Kamali-Moghaddam M. Elevated Serum GAD65 and GAD65-GADA Immune Complexes in Stiff Person Syndrome. Scientific Reports 2015, 5: 11196. PMID: 26080009, PMCID: PMC4468815, DOI: 10.1038/srep11196.Peer-Reviewed Original ResearchConceptsStiff-person syndromeImmune complexesPerson syndromeGlutamic acid decarboxylase 65Type 1 diabetesProximity ligation assayAutoimmune diseasesHealthy controlsSP patientsBlood samplesLigation assayClinical diagnosisPg/GAD65Serum samplesImproved diagnosisSyndromeSpecific assayDiseaseDiagnosisPresent studyGADAAssays