2007
Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect
Clark PR, Pober JS, Kluger MS. Knockdown of TNFR1 by the sense strand of an ICAM-1 siRNA: dissection of an off-target effect. Nucleic Acids Research 2007, 36: 1081-1097. PMID: 18096615, PMCID: PMC2275081, DOI: 10.1093/nar/gkm630.Peer-Reviewed Original ResearchConceptsTumor necrosis factor receptor 1Endothelial cellsIntercellular adhesion molecule-1ICAM-1 siRNAAnti-TNF activityCultured human umbilical vein endothelial cellsICAM-1 expressionAdhesion molecule-1Off-target effectsNecrosis factor receptor 1Human umbilical vein ECHuman umbilical vein endothelial cellsFactor receptor 1Umbilical vein endothelial cellsExpression of mRNAIL-1 activationVein endothelial cellsLocal inflammationTNFR1 mRNANecrosis factorEarly TNFICAM-1VCAM-1E-selectinTransfection of ECs
2006
Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations
Clark PR, Manes TD, Pober JS, Kluger MS. Increased ICAM-1 Expression Causes Endothelial Cell Leakiness, Cytoskeletal Reorganization and Junctional Alterations. Journal Of Investigative Dermatology 2006, 127: 762-774. PMID: 17195014, DOI: 10.1038/sj.jid.5700670.Peer-Reviewed Original ResearchConceptsEC barrier functionCell junctionsHuman dermal microvascular ECsSmall-interfering RNA knockdownEC shape changesEndothelial cellsG actin ratioActin cytoskeletonShape changesCytoskeleton organizationCell elongationICAM-1 inductionCytoskeletal reorganizationIntracellular regionCell shapeRNA knockdownHuman dermal microvascular endothelial cellsDermal microvascular endothelial cellsZona occludens-1NF-kappaB activationJunctional alterationsCytoskeletonBarrier functionCell leakinessMicrovascular endothelial cells