A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes
Mandal A, Moneme C, Tewari B, Goldstein A, Sontheimer H, Cheng L, Moore S, Levin D. A novel method for culturing enteric neurons generates neurospheres containing functional myenteric neuronal subtypes. Journal Of Neuroscience Methods 2024, 407: 110144. PMID: 38670535, PMCID: PMC11144385, DOI: 10.1016/j.jneumeth.2024.110144.Peer-Reviewed Original ResearchEnteric neuronsEnteric nervous systemPresence of sodium channel blockersProgenitor cellsProgenitor cell marker nestinAction potentialsNeural progenitor cell markers nestinNeural stemMyenteric plexus cellsActivate enteric neuronsCultured enteric neuronsCells expressing Sox2Differentiation culture systemSodium channel blockersMurine small intestineNeural progenitor cellsTubulin beta IIIDays of differentiationChannel blockersDifferentiated neurospheresNeuN immunofluorescenceEnteric neurospheresNeuronal subtypesMarker nestinPlexus cells