2018
MIWI2 targets RNAs transcribed from piRNA‐dependent regions to drive DNA methylation in mouse prospermatogonia
Watanabe T, Cui X, Yuan Z, Qi H, Lin H. MIWI2 targets RNAs transcribed from piRNA‐dependent regions to drive DNA methylation in mouse prospermatogonia. The EMBO Journal 2018, 37: embj201695329. PMID: 30108053, PMCID: PMC6138435, DOI: 10.15252/embj.201695329.Peer-Reviewed Original ResearchConceptsDNA methylationRetrotransposon sequencesSmall RNAsArgonaute/Piwi proteinsPiwi protein MIWI2Suppressive epigenetic marksMouse prospermatogoniaChromatin statePIWI proteinsUnderlying molecular mechanismsDiverse organismsEpigenetic marksPiRNA clustersNascent RNAEpigenetic regulationTranslational regulationMIWI2RNA degradationRepeat sequencesGene expressionMolecular mechanismsTarget RNAMethylationRNAPiRNAs
2016
An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline1
Mak W, Fang C, Holden T, Dratver MB, Lin H. An Important Role of Pumilio 1 in Regulating the Development of the Mammalian Female Germline1. Biology Of Reproduction 2016, 94: 134, 1-11. PMID: 27170441, PMCID: PMC4946805, DOI: 10.1095/biolreprod.115.137497.Peer-Reviewed Original ResearchConceptsPumilio 1PUF proteinsLate meiotic prophase IPumilio/FBF (PUF) proteinReproductive competencyPrimordial follicle poolFemale germ cell developmentMammalian germ cellsPrimordial folliculogenesisFemale reproductive competenceMeiotic prophase IGerm cell developmentFBF proteinsGermline establishmentTranslational regulatorTranslational regulationProphase IDiplotene stageCell developmentGerm cellsFollicle poolImportant roleDetectable functionMammalsReproductive competence
2006
The Role of PIWI and the miRNA Machinery in Drosophila Germline Determination
Megosh HB, Cox DN, Campbell C, Lin H. The Role of PIWI and the miRNA Machinery in Drosophila Germline Determination. Current Biology 2006, 16: 1884-1894. PMID: 16949822, DOI: 10.1016/j.cub.2006.08.051.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsArgonaute ProteinsBody PatterningCell DifferentiationDEAD-box RNA HelicasesDrosophilaDrosophila ProteinsEmbryo, NonmammalianEmbryonic DevelopmentFragile X Mental Retardation ProteinGene Expression Regulation, DevelopmentalGerm CellsMicroRNAsProteinsRibonuclease IIIRNA HelicasesRNA-Induced Silencing ComplexRNA, Small InterferingConceptsGermline determinationDicer-1Dicer-2MiRNA pathwayRole of PiwiReduced PGC numbersNumber of PGCsMiRISC complexesRasiRNA pathwayGermline specificationDrosophila PiwiSiRNA pathwayAbdominal patterningVasa expressionTranslational regulationPGC numberPiwiMiRNA machineryGerm plasmMolecular activityPolar granuleDFMRPCell formationOSKMaternal componentA novel class of small RNAs in mouse spermatogenic cells
Grivna ST, Beyret E, Wang Z, Lin H. A novel class of small RNAs in mouse spermatogenic cells. Genes & Development 2006, 20: 1709-1714. PMID: 16766680, PMCID: PMC1522066, DOI: 10.1101/gad.1434406.Peer-Reviewed Original ResearchConceptsPIWI-interacting RNAsSmall RNAsMouse male germlineArgonaute protein familyPost-transcriptional levelSmall noncoding RNAsMouse spermatogenic cellsDiverse organismsMale germlineTranslational regulationProtein familyNoncoding RNAsSubfamily membersGene expressionMicro RNAsNovel classRNAPotent regulatorMIWISpermatogenic cellsPotential roleBiogenesisGermlinePolysomesOrganisms
2004
Mili, a mammalian member of piwi family gene, is essential for spermatogenesis
Kuramochi-Miyagawa S, Kimura T, Ijiri TW, Isobe T, Asada N, Fujita Y, Ikawa M, Iwai N, Okabe M, Deng W, Lin H, Matsuda Y, Nakano T. Mili, a mammalian member of piwi family gene, is essential for spermatogenesis. Development 2004, 131: 839-849. PMID: 14736746, DOI: 10.1242/dev.00973.Peer-Reviewed Original ResearchConceptsPiwi family genesFamily genesPrimordial germ cell developmentPost-transcriptional regulationGerm cell developmentGerm cell productionPhysical binding propertiesMammalian homologPIWI domainMammalian membersTranslational regulationMouse homologDifferentiation of spermatocytesEarly prophaseMILIFunctional associationTargeted mutationsCell developmentFirst meiosisGenesPiwiEarly pachyteneStem cellsSpermatocyte stageHomolog
2002
miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis
Deng W, Lin H. miwi, a Murine Homolog of piwi, Encodes a Cytoplasmic Protein Essential for Spermatogenesis. Developmental Cell 2002, 2: 819-830. PMID: 12062093, DOI: 10.1016/s1534-5807(02)00165-x.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsArgonaute ProteinsChromosome MappingCloning, MolecularCyclic AMP Response Element ModulatorCytoplasmDNA-Binding ProteinsGene Expression RegulationInfertility, MaleLIM Domain ProteinsMaleMiceMice, KnockoutMolecular Sequence DataProtein IsoformsProteinsRepressor ProteinsRNA, MessengerSpermatogenesisTestisTranscription FactorsConceptsTarget genesPiwi family genesCytoplasmic protein essentialRound spermatid stageDiverse organismsPiwi genesTranslational regulationFamily genesProtein essentialCytoplasmic proteinsMaster regulatorMurine homologMIWIGenesSpermatid stageStem cellsMRNASpermiogenesisCREMSpermatogenesisSpermatogenic arrestPiwiHomologEncodesCloning