2000
Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways
Goodwin E, DiMaio D. Repression of human papillomavirus oncogenes in HeLa cervical carcinoma cells causes the orderly reactivation of dormant tumor suppressor pathways. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 12513-12518. PMID: 11070078, PMCID: PMC18795, DOI: 10.1073/pnas.97.23.12513.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBovine papillomavirus 1Carrier ProteinsCattleCell Cycle ProteinsCyclin-Dependent Kinase Inhibitor p21CyclinsDNADNA-Binding ProteinsE2F Transcription FactorsFemaleGene Expression Regulation, ViralGenes, Tumor SuppressorHeLa CellsHumansNuclear ProteinsOncogene Proteins, ViralOncogenesPapillomaviridaePapillomavirus E7 ProteinsPhosphoproteinsProteinsProto-Oncogene ProteinsProto-Oncogene Proteins c-mdm2Repressor ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Retinoblastoma-Like Protein p107Retinoblastoma-Like Protein p130Signal TransductionTranscription Factor DP1Transcription FactorsTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsTumor suppressor pathwayE6/E7 repressionPosttranscriptional inductionSuppressor pathwayBovine papillomavirus E2 proteinE7 repressionCyclin-dependent kinase activityHeLa cellsE2F-regulated genesE2F-responsive genesRb tumor suppressor pathwayPapillomavirus E2 proteinCell cycle machineryE2 proteinHPV16 E6/E7 genesHeLa cervical carcinoma cellsP53-responsive genesTumor suppressor functionHPV E6Growth inhibitory signalsE6/E7 genesRapid repressionCellular DNA synthesisCycle machineryHuman papillomavirus oncogenesE2F-Rb Complexes Assemble and Inhibit cdc25A Transcription in Cervical Carcinoma Cells following Repression of Human Papillomavirus Oncogene Expression
Wu L, Goodwin E, Naeger L, Vigo E, Galaktionov K, Helin K, DiMaio D. E2F-Rb Complexes Assemble and Inhibit cdc25A Transcription in Cervical Carcinoma Cells following Repression of Human Papillomavirus Oncogene Expression. Molecular And Cellular Biology 2000, 20: 7059-7067. PMID: 10982822, PMCID: PMC86242, DOI: 10.1128/mcb.20.19.7059-7067.2000.Peer-Reviewed Original ResearchMeSH KeywordsBinding SitesBovine papillomavirus 1Carcinoma, Squamous CellCarrier ProteinsCdc25 PhosphatasesCell CycleCell Cycle ProteinsCell Transformation, NeoplasticCell Transformation, ViralConsensus SequenceCysteine EndopeptidasesDNA, NeoplasmDNA-Binding ProteinsE2F Transcription FactorsE2F4 Transcription FactorFemaleGene Expression Regulation, NeoplasticGene Expression Regulation, ViralGenes, RetinoblastomaHumansMacromolecular SubstancesMultienzyme ComplexesNeoplasm ProteinsPapillomaviridaePapillomavirus InfectionsPhosphoproteinsPromoter Regions, GeneticProteasome Endopeptidase ComplexProtein BindingProteinsRecombinant Fusion ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Retinoblastoma-Like Protein p130Transcription Factor DP1Transcription FactorsTransfectionTumor Cells, CulturedTumor Virus InfectionsUterine Cervical NeoplasmsViral ProteinsConceptsCdc25A promoterE6/E7 repressionCervical carcinoma cellsE2F siteBovine papillomavirus E2 proteinE2 proteinE7 repressionWild-type E2 proteinE2F-responsive promotersRb tumor suppressor pathwayPapillomavirus E2 proteinCarcinoma cellsE2F-Rb complexesCell cycle genesHuman papillomavirus oncogene expressionHuman papillomavirus (HPV) E6/E7 oncogenesTumor suppressor pathwayMechanism of repressionHPV E6/E7 expressionCell cycle progressionCdc25A transcriptionDramatic growth arrestE2F complexesConsensus E2FProtein complexes
1999
Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein.
Naeger L, Goodwin E, Hwang E, DeFilippis R, Zhang H, DiMaio D. Bovine papillomavirus E2 protein activates a complex growth-inhibitory program in p53-negative HT-3 cervical carcinoma cells that includes repression of cyclin A and cdc25A phosphatase genes and accumulation of hypophosphorylated retinoblastoma protein. Molecular Cancer Research 1999, 10: 413-22. PMID: 10392903.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarrier ProteinsCattleCDC2-CDC28 KinasesCdc25 PhosphatasesCell Cycle ProteinsCell DivisionCyclin ACyclin-Dependent Kinase 2Cyclin-Dependent Kinase Inhibitor p21Cyclin-Dependent KinasesCyclinsDNA-Binding ProteinsE2F Transcription FactorsE2F1 Transcription FactorEnzyme InhibitorsFemaleGene Expression RegulationGrowth InhibitorsHeLa CellsHumansPhosphoprotein PhosphatasesPhosphorylationProtein Serine-Threonine KinasesProtein Tyrosine PhosphatasesRepressor ProteinsRetinoblastoma ProteinRetinoblastoma-Binding Protein 1Transcription Factor DP1Transcription FactorsTumor Cells, CulturedTumor Suppressor Protein p53Uterine Cervical NeoplasmsViral ProteinsConceptsCervical carcinoma cell linesHT-3 cellsCarcinoma cell linesE2 proteinE6/E7 genesCell linesGrowth inhibitionCervical carcinoma cellsCyclin ACyclin-dependent kinase 2 activityExpression of CDC25AKinase 2 activityE7 genesCell cycle componentsCarcinoma cellsCDC25B expressionE2 expressionProtein levels