2020
A six-amino-acid motif is a major determinant in functional evolution of HOX1 proteins
Singh N, De Kumar B, Paulson A, Parrish M, Zhang Y, Florens L, Conaway J, Si K, Krumlauf R. A six-amino-acid motif is a major determinant in functional evolution of HOX1 proteins. Genes & Development 2020, 34: 1680-1696. PMID: 33184220, PMCID: PMC7706710, DOI: 10.1101/gad.342329.120.Peer-Reviewed Original ResearchConceptsC-terminal motifAncestral activityFunctional analysisDNA-binding patternsGenome-wide analysisDNA-binding regionLow sequence similarityDNA-binding experimentsAmino acid sequenceSix-amino acid motifAncestral functionPbx proteinsEvolutionary noveltyGene duplicationFunctional diversificationHoxa1 proteinMouse proteinProtein functionSequence similarityFunctional evolutionAcid sequenceChimeric proteinMouse cellsHoxb1Structural predictions
2019
A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates
Parker H, De Kumar B, Green S, Prummel K, Hess C, Kaufman C, Mosimann C, Wiedemann L, Bronner M, Krumlauf R. A Hox-TALE regulatory circuit for neural crest patterning is conserved across vertebrates. Nature Communications 2019, 10: 1189. PMID: 30867425, PMCID: PMC6416258, DOI: 10.1038/s41467-019-09197-8.Peer-Reviewed Original ResearchConceptsNeural crestHox genesAncestral mechanismRegulatory circuitsUpstream regulatory componentsNeural crest patterningAncient paraloguesPbx proteinsJawed vertebratesAncient conservationExtant vertebratesAncestral activityRegulatory interactionsHOX2 genesRegulatory componentsVertebratesJaw formationGenesGnathostomesEnhancerHoxLampreyParaloguesImportant roleHoxa2