2021
A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin.
Brown S, Ren Y, Suter D, Mattoo S. A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin. Bio-protocol 2021, 11: e4158. PMID: 34692908, PMCID: PMC8481015, DOI: 10.21769/bioprotoc.4158.Peer-Reviewed Original ResearchPhosphorylation of cortactinActin cytoskeletal dynamicsNon-phosphorylated proteinActin-binding proteinsRecombinant protein expressionCell-free systemTumor cell metastasisNeuronal growth conesBacterial kinasesLamellipodia protrusionBag cell neuronsPhosphorylation assaysNon-neuronal cellsFilopodia formationSrc kinaseInduced proteinsCortactinTyrosine kinaseEfficient expressionKinaseCell migrationPhosphorylationBiochemical studiesCell metastasisGrowth cones
2015
Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones
He Y, Ren Y, Wu B, Decourt B, Lee A, Taylor A, Suter D. Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones. Molecular Biology Of The Cell 2015, 26: 3229-3244. PMID: 26224308, PMCID: PMC4569314, DOI: 10.1091/mbc.e15-03-0142.Peer-Reviewed Original ResearchConceptsActin network densityGrowth conesSrc tyrosine kinaseFluorescent speckle microscopyState of actinNeuronal growth conesGrowth cone motilityLamellipodia protrusionActin organizationActin assemblyFilopodia formationActin bundlesAplysia growth conesSpeckle microscopyTyrosine kinaseUnderlying cellular mechanismsFilopodial densityCone motilityCellular mechanismsSrcFilopodiaActivation stateLamellipodiaSRC2Axonal growth