2023
Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels
Shen Q, Feng Q, Wu C, Xiong Q, Tian T, Yuan S, Shi J, Bedwell G, Yang R, Aiken C, Engelman A, Lusk C, Lin C, Xiong Y. Modeling HIV-1 nuclear entry with nucleoporin-gated DNA-origami channels. Nature Structural & Molecular Biology 2023, 30: 425-435. PMID: 36807645, PMCID: PMC10121901, DOI: 10.1038/s41594-023-00925-9.Peer-Reviewed Original ResearchConceptsNuclear pore complexHIV-1 nuclear entryNuclear entryNuclear importNPC central channelPore complexHost nucleusCapsid dockingVirus genomeAffinity gradientNup153Central channelMechanistic insightsMolecular interactionsCapsidNucleoporinsNup358Nup62GenomeNucleusVirusDockingVirus-1 infectionImportComplexes
2022
Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity
Shen Q, Xiong Q, Zhou K, Feng Q, Liu L, Tian T, Wu C, Xiong Y, Melia T, Lusk C, Lin C. Functionalized DNA-Origami-Protein Nanopores Generate Large Transmembrane Channels with Programmable Size-Selectivity. Journal Of The American Chemical Society 2022, 145: 1292-1300. PMID: 36577119, PMCID: PMC9852090, DOI: 10.1021/jacs.2c11226.Peer-Reviewed Original ResearchConceptsExchange of macromoleculesCholesterol-rich membranesHybrid nanoporesSynthetic biologyBiophysical toolsSynthetic cellsTransmembrane channelsTransmembrane nanoporesDNA ringsProtein nanoporeCell membraneBacterial toxinsDNA origami techniqueLipid membranesAnalytical chemistryMacromolecule sizeDNA origamiMembraneProgrammable sizeNanoporesSized poresNucleoporinsAverage inner diameterCellsPneumolysin
2001
X-ray analysis of an RNA tetraplex (UGGGGU)4 with divalent Sr2+ ions at subatomic resolution (0.61 Å)
Deng J, Xiong Y, Sundaralingam M. X-ray analysis of an RNA tetraplex (UGGGGU)4 with divalent Sr2+ ions at subatomic resolution (0.61 Å). Proceedings Of The National Academy Of Sciences Of The United States Of America 2001, 98: 13665-13670. PMID: 11707581, PMCID: PMC61098, DOI: 10.1073/pnas.241374798.Peer-Reviewed Original ResearchCrystal structures of two forms of a 14-mer RNA/DNA chimer duplex with double UU bulges: a novel intramolecular U*(A x U) base triple.
Deng J, Xiong Y, Sudarsanakumar C, Shi K, Sundaralingam M. Crystal structures of two forms of a 14-mer RNA/DNA chimer duplex with double UU bulges: a novel intramolecular U*(A x U) base triple. RNA 2001, 7: 1425-31. PMID: 11680847, PMCID: PMC1370186.Peer-Reviewed Original Research
2000
Crystal structure of a DNA·RNA hybrid duplex with a polypurine RNA r(gaagaagag) and a complementary polypyrimidine DNA d(CTCTTCTTC)
Xiong Y, Sundaralingam M. Crystal structure of a DNA·RNA hybrid duplex with a polypurine RNA r(gaagaagag) and a complementary polypyrimidine DNA d(CTCTTCTTC). Nucleic Acids Research 2000, 28: 2171-2176. PMID: 10773088, PMCID: PMC105378, DOI: 10.1093/nar/28.10.2171.Peer-Reviewed Original ResearchCrystal structure of an adenine bulge in the RNA chain of a DNA·RNA hybrid, d(CTCCTCTTC)·r(gaagagagag)11Edited by I. Tinoco
Sudarsanakumar C, Xiong Y, Sundaralingam M. Crystal structure of an adenine bulge in the RNA chain of a DNA·RNA hybrid, d(CTCCTCTTC)·r(gaagagagag)11Edited by I. Tinoco. Journal Of Molecular Biology 2000, 299: 103-112. PMID: 10860725, DOI: 10.1006/jmbi.2000.3730.Peer-Reviewed Original Research