2022
Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume
Du Z, Yan Q, Shen E, Weinstein A, Wang T. Regulation of glomerulotubular balance. IV. Implication of aquaporin 1 in flow-dependent proximal tubule transport and cell volume. American Journal Of Physiology. Renal Physiology 2022, 323: f642-f653. PMID: 36108052, PMCID: PMC9705020, DOI: 10.1152/ajprenal.00167.2022.Peer-Reviewed Original ResearchConceptsWater channel aquaporin-1Proximal tubulesKO miceAquaporin-1Water reabsorptionMouse PTNo significant differenceWild-typeTight junctionsRat kidneyAquaporin-1 knockoutSignificant differenceAcid-base parametersKidney proximal tubulesRenal clearance experimentsWT miceKnockout miceUrine flowFlow stimulationFluid absorptionUrine pHClearance experimentsMouse kidneyMiceRats
2021
Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice
Xu S, Li J, Yang L, Wang CJ, Liu T, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport III: Impact of low K intake on thiazide-sensitive cation excretion in male and female mice. Pflügers Archiv - European Journal Of Physiology 2021, 473: 1749-1760. PMID: 34455480, PMCID: PMC8528772, DOI: 10.1007/s00424-021-02611-5.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateFemale miceUrine volumeNaCl cotransporterLow K intakeMeasurement of functionSex differencesNatriuretic responseFractional excretionK intakeTransport protein expressionFiltration rateNCC abundanceNCC expressionRenal clearanceDistal nephronLK dietCation excretionPlasma KNCC proteinNKCC2 expressionWestern blottingProtein expressionMiceFENaRestoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease
Du Z, Tian X, Ma M, Somlo S, Weinstein AM, Wang T. Restoration of proximal tubule flow-activated transport prevents cyst growth in polycystic kidney disease. JCI Insight 2021, 6: e146041. PMID: 33886508, PMCID: PMC8262298, DOI: 10.1172/jci.insight.146041.Peer-Reviewed Original ResearchConceptsGlomerular filtration rateGlomerulotubular balanceRenal cyst formationCyst formationReceptor 1 antagonistPolycystic kidney diseaseKidney weightUntreated miceDA1 antagonistControl miceKidney diseaseFiltration rateFractional reabsorptionCystic indexMouse modelCyst growthConditional KOHCO3- absorptionHeterozygous miceSame antagonistsMicePT transportAntagonistEpithelial ciliaHCO3- transportDeletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse
Thomson R, Dynia DW, Burlein S, Thomson BR, Booth C, Knauf F, Wang T, Aronson P. Deletion of Cdh16 Ksp-cadherin leads to a developmental delay in the ability to maximally concentrate urine in mouse. American Journal Of Physiology. Renal Physiology 2021, 320: f1106-f1122. PMID: 33938239, PMCID: PMC8285649, DOI: 10.1152/ajprenal.00556.2020.Peer-Reviewed Original ResearchConceptsKsp-cadherinCell adhesion moleculeAtypical memberKidney developmentMammalian kidneyAdult mammalian kidneyBasolateral membraneNormal kidney developmentEpithelial cellsAdhesion moleculesMutant animalsExpression analysisSpecific expressionE-cadherin expressionWestern blot analysisEpithelial phenotypePrincipal proteinE-cadherinBlot analysisMouse linesAquaporin-2CadherinCritical roleDevelopmental delayKnockout mice
2019
Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice
Li J, Xu S, Yang L, Yang J, Wang CJ, Weinstein AM, Palmer LG, Wang T. Sex difference in kidney electrolyte transport II: impact of K+ intake on thiazide-sensitive cation excretion in male and female mice. American Journal Of Physiology. Renal Physiology 2019, 317: f967-f977. PMID: 31390232, PMCID: PMC6843050, DOI: 10.1152/ajprenal.00125.2019.Peer-Reviewed Original ResearchConceptsHK dietCation excretionWT animalsFemale animalsMale animalsSex differencesRenal clearance experimentsGlomerular filtration rateR KO miceExchanger isoform 3Distal NaHK intakeWestern blot analysisFractional excretionUrine volumeFiltration rateFemale miceKO miceNHE3 abundanceClearance experimentsNCC abundanceLittle sex differenceKnockout miceENaC expressionExcretion
2018
Regulation of renal Na transporters in response to dietary K
Yang L, Xu S, Guo X, Uchida S, Weinstein AM, Wang T, Palmer LG. Regulation of renal Na transporters in response to dietary K. American Journal Of Physiology. Renal Physiology 2018, 315: f1032-f1041. PMID: 29923764, PMCID: PMC6230734, DOI: 10.1152/ajprenal.00117.2018.Peer-Reviewed Original Research
2016
Kidney Tubular Ablation of Ocrl/Inpp5b Phenocopies Lowe Syndrome Tubulopathy
Inoue K, Balkin DM, Liu L, Nandez R, Wu Y, Tian X, Wang T, Nussbaum R, De Camilli P, Ishibe S. Kidney Tubular Ablation of Ocrl/Inpp5b Phenocopies Lowe Syndrome Tubulopathy. Journal Of The American Society Of Nephrology 2016, 28: 1399-1407. PMID: 27895154, PMCID: PMC5407733, DOI: 10.1681/asn.2016080913.Peer-Reviewed Original ResearchConceptsEarly embryonic lethalityTransporter 5Dent's diseaseIndependent endocytosisEmbryonic lethalityRedundant functionsType 2 inositolHuman phenotypesProximal tubule endocytosisOculocerebrorenal syndromeGenetic ablationCellular levelGermline knockoutLowe syndromeEndocytosisMouse backgroundMice resultsMutationsInositolLow molecular weight proteinuriaINPP5BParalogsProximal tubule functionDramatic effectOCRLPotassium Channelopathies and Gastrointestinal Ulceration
Han J, Lee SH, Giebisch G, Wang T. Potassium Channelopathies and Gastrointestinal Ulceration. Gut And Liver 2016, 10: 881-889. PMID: 27784845, PMCID: PMC5087926, DOI: 10.5009/gnl15414.Peer-Reviewed Original ResearchConceptsPotassium channelsGI tractPotassium channelopathiesAdenosine triphosphate-sensitive potassium channel openerTriphosphate-sensitive potassium channel openerNonsteroidal anti-inflammatory drugsAntianginal drug nicorandilAnti-inflammatory drugsPotassium channel openersOccurrence of ulcerationPotassium channel activityLong-term useRegulation of secretionGastrointestinal ulcerationUlcerative colitisPeptic ulcerationChannel openersGastric acidGastrointestinal tractUlcerationDifferent biological actionsTransporter inhibitorsAnal regionPotassium homeostasisTract
2014
Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1
Vucic E, Alfadda T, MacGregor GG, Dong K, Wang T, Geibel JP. Kir1.1 (ROMK) and Kv7.1 (KCNQ1/KvLQT1) are essential for normal gastric acid secretion: importance of functional Kir1.1. Pflügers Archiv - European Journal Of Physiology 2014, 467: 1457-1468. PMID: 25127675, DOI: 10.1007/s00424-014-1593-0.Peer-Reviewed Original ResearchConceptsGastric parietal cellsPotassium channelsParietal cellsΒ-subunitKir1.1 channelsWild-type miceSecretagogue-stimulated gastric acid secretionApical poleGastric glandsLeak pathwayPotential therapeutic targetKir1.1Proton secretionRegulatory characteristicsKv7.1Therapeutic targetATPaseCell numberParietal cell numberCellsNormal gastric acid secretionSecretionInhibitorsAcid secretionMice
2013
Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation
Pluznick JL, Protzko RJ, Gevorgyan H, Peterlin Z, Sipos A, Han J, Brunet I, Wan LX, Rey F, Wang T, Firestein SJ, Yanagisawa M, Gordon JI, Eichmann A, Peti-Peterdi J, Caplan MJ. Olfactory receptor responding to gut microbiota-derived signals plays a role in renin secretion and blood pressure regulation. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 4410-4415. PMID: 23401498, PMCID: PMC3600440, DOI: 10.1073/pnas.1215927110.Peer-Reviewed Original ResearchConceptsShort-chain fatty acidsRenin secretionBlood pressureGut microbiotaG protein-coupled receptor 41Acute hypotensive responseRenal juxtaglomerular apparatusSmall resistance vesselsMicrobiota-derived signalsModulate blood pressureBlood pressure regulationWild-type miceSmooth muscle cellsG protein-coupled receptorsGPR41 expressionOlfactory receptorsHypotensive responseProtein-coupled receptorsSCFA receptorsResistance vesselsJuxtaglomerular apparatusAntibiotic treatmentOlfr78Receptor 41Knockout mice
2012
Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport
Du Z, Wan L, Yan Q, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. II. Impact of angiotensin II on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f1507-f1516. PMID: 22952281, PMCID: PMC3532483, DOI: 10.1152/ajprenal.00277.2012.Peer-Reviewed Original ResearchAngiotensin IIAngiotensin II Type 1 Receptor BlockersAnimalsBicarbonatesBiological TransportEnzyme InhibitorsFemaleHemostasisIn Vitro TechniquesKidney GlomerulusKidney TubulesLosartanMacrolidesMiceMice, KnockoutModels, AnimalProton-Translocating ATPasesReceptor, Angiotensin, Type 1SodiumSodium-Hydrogen Exchanger 3Sodium-Hydrogen ExchangersRegulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport
Du Z, Yan Q, Wan L, Weinbaum S, Weinstein A, Wang T. Regulation of glomerulotubular balance. I. Impact of dopamine on flow-dependent transport. American Journal Of Physiology. Renal Physiology 2012, 303: f386-f395. PMID: 22552936, PMCID: PMC3433864, DOI: 10.1152/ajprenal.00531.2011.Peer-Reviewed Original Research8-Bromo Cyclic Adenosine MonophosphateAlgorithmsAnimalsBenzazepinesBicarbonatesBiological Transport, ActiveChloridesCyclic AMP-Dependent Protein KinasesDopamineDopamine AntagonistsDopamine D2 Receptor AntagonistsFemaleIsoquinolinesKidney GlomerulusKidney TubulesKidney Tubules, ProximalMacrolidesMiceMicrovilliProton-Translocating ATPasesReceptors, Dopamine D1SodiumSulfonamidesSulpiride
2011
Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction
Wang T. Renal outer medullary potassium channel knockout models reveal thick ascending limb function and dysfunction. Clinical And Experimental Nephrology 2011, 16: 49-54. PMID: 22038261, DOI: 10.1007/s10157-011-0495-0.Peer-Reviewed Original ResearchConceptsThick ascending limbIon transporter expressionRenal outer medullary potassium channelBartter's syndromeInward rectifier potassium channelPotassium channelsSmall-conductance K channelsROMK null miceMedullary thick ascending limbType II Bartter's syndromeSimilar phenotypeMammalian kidneyApical membraneK channelsROMK knockout miceKnockout modelsChannel activityChannel mutationsRenal functionLimb functionNull micePhysiological conditionsSalt wastingTransporter expressionPathophysiological conditionsChloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*
Lin Z, Jin S, Duan X, Wang T, Martini S, Hulamm P, Cha B, Hubbard A, Donowitz M, Guggino SE. Chloride Channel (Clc)-5 Is Necessary for Exocytic Trafficking of Na+/H+ Exchanger 3 (NHE3)*. Journal Of Biological Chemistry 2011, 286: 22833-22845. PMID: 21561868, PMCID: PMC3123051, DOI: 10.1074/jbc.m111.224998.Peer-Reviewed Original ResearchConceptsKO miceTrafficking of NHE3Proximal tubulesOpossum kidney cellsNHE3 activityDent's diseaseClC-5Surface expressionNHE3 surface expressionKidney cellsRenal proximal tubulesTotal protein levelsChloride/proton exchangerRates of basalReduced surface expressionKnockdown cellsParathyroid hormoneWT miceDegree of inhibitionCLCN5 geneSurface NHE3MiceTubule perfusionReduced expressionTwo-photon microscopyDifferential regulation of ROMK (Kir1.1) in distal nephron segments by dietary potassium
Wade JB, Fang L, Coleman RA, Liu J, Grimm PR, Wang T, Welling PA. Differential regulation of ROMK (Kir1.1) in distal nephron segments by dietary potassium. American Journal Of Physiology. Renal Physiology 2011, 300: f1385-f1393. PMID: 21454252, PMCID: PMC3119145, DOI: 10.1152/ajprenal.00592.2010.Peer-Reviewed Original ResearchConceptsDistal nephronDistal nephron segmentsRenal K secretionROMK knockout miceDietary potassiumExtent of expressionKnockout miceK secretionROMK expressionNephron segmentsNew antibodiesApical labelingPhysiological stimuliNephronROMKAntibodiesROMK channelsApical expressionChannel localizationChannel functionDifferential regulationLarge increaseDCT2Different regulatory mechanismsExtensive pattern
2010
Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells
Marin EP, Moeckel G, Al-Lamki R, Bradley J, Yan Q, Wang T, Wright PL, Yu J, Sessa WC. Identification and Regulation of Reticulon 4B (Nogo-B) in Renal Tubular Epithelial Cells. American Journal Of Pathology 2010, 177: 2765-2773. PMID: 20971739, PMCID: PMC2993268, DOI: 10.2353/ajpath.2010.100199.Peer-Reviewed Original ResearchConceptsUnilateral ureteral obstructionAcute tubular necrosisEpithelial cellsRenal tubular epithelial cellsMurine kidneyIschemia/reperfusionMeasurement of fibrosisDistal nephron segmentsRecruitment of macrophagesWild-type miceInflammatory gene expressionTubular epithelial cellsDe novo expressionHuman biopsy specimensRenal injuryTubular necrosisUreteral obstructionWT miceVascular injuryHistological damageBiopsy specimensCortical tubulesDeficient miceMacrophage recruitmentTissue injuryAltered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI
Gotoh N, Yan Q, Du Z, Biemesderfer D, Kashgarian M, Mooseker MS, Wang T. Altered renal proximal tubular endocytosis and histology in mice lacking myosin‐VI. Cytoskeleton 2010, 67: 178-192. PMID: 20175219, PMCID: PMC3468331, DOI: 10.1002/cm.20435.Peer-Reviewed Original ResearchMeSH KeywordsActinsAdaptor Proteins, Signal TransducingAdaptor Proteins, Vesicular TransportAnimalsApoptosis Regulatory ProteinsCell TransdifferentiationEndocytosisHorseradish PeroxidaseKidney Tubules, ProximalLow Density Lipoprotein Receptor-Related Protein-2MiceMice, Neurologic MutantsMicrofilament ProteinsMicrovilliMyosin Heavy ChainsVesicular Transport ProteinsConceptsProximal tubule cellsAlbumin excretionSv miceTubule cellsProximal tubule brush borderGlomerular flow rateProximal tubular dilationUrinary albumin excretionElevated blood pressureReduced body weightProximal tubular endocytosisRenal proximal tubule brush borderTubule brush borderProximal tubular uptakeBlood acid-base parametersMouse kidney proximal tubulesKidney proximal tubulesBlood pressureUrine volumeUrinary excretionAcid-base parametersRenal homogenatesTubular dilationPlasma concentrationsRenal clearance
2009
Functional expression of the olfactory signaling system in the kidney
Pluznick JL, Zou DJ, Zhang X, Yan Q, Rodriguez-Gil DJ, Eisner C, Wells E, Greer CA, Wang T, Firestein S, Schnermann J, Caplan MJ. Functional expression of the olfactory signaling system in the kidney. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 2059-2064. PMID: 19174512, PMCID: PMC2644163, DOI: 10.1073/pnas.0812859106.Peer-Reviewed Original ResearchConceptsGlomerular filtration ratePlasma renin levelsMacula densa cellsCOX-2 expressionRenal distal nephronOlfactory G-proteinMDS cell linesOlfactory receptorsRenin levelsRenin secretionFiltration rateNNOS activityTubuloglomerular feedbackDistal nephronOlfactory epitheliumRenal tubulesGFR regulationAdenylate cyclaseG proteinsCell linesSensory roleKidneyFunctional expressionOlfactionExpression
2008
Disruption of Myosin 1e Promotes Podocyte Injury
Krendel M, Kim SV, Willinger T, Wang T, Kashgarian M, Flavell RA, Mooseker MS. Disruption of Myosin 1e Promotes Podocyte Injury. Journal Of The American Society Of Nephrology 2008, 20: 86-94. PMID: 19005011, PMCID: PMC2615733, DOI: 10.1681/asn.2007111172.Peer-Reviewed Original ResearchConceptsChronic renal injuryNormal glomerular filtrationGlomerular basement membraneRenal injuryKidney inflammationPodocyte foot processesGlomerular diseasePodocyte injuryGlomerular filtrationRenal tissueFoot processesPodocyte functionBasement membraneInjuryUltrastructural levelDiseaseMiceType IProteinuriaInflammationKidneyImportant rolePodocytesNeutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo
Wei F, Karihaloo A, Yu Z, Marlier A, Seth P, Shibazaki S, Wang T, Sukhatme VP, Somlo S, Cantley LG. Neutrophil gelatinase-associated lipocalin suppresses cyst growth by Pkd1 null cells in vitro and in vivo. Kidney International 2008, 74: 1310-1318. PMID: 18974761, PMCID: PMC3793389, DOI: 10.1038/ki.2008.395.Peer-Reviewed Original ResearchConceptsNeutrophil Gelatinase-Associated LipocalinCyst growthGelatinase-associated lipocalinAutosomal dominant polycystic kidney diseaseTubular cell proliferationDominant polycystic kidney diseaseCell linesPolycystic kidney diseasePkd1 mutant miceRenal functionAdenoviral delivery systemAssociated lipocalinKidney diseaseRate of proliferationCyst enlargementProliferative responseRenal epithelial cellsProximal tubulesMutant miceEpithelial cellsFlow stimuliCell proliferationDecreased rateApical ciliaProliferation