2023
Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing
McCool M, Bryant C, Huang H, Ogawa L, Farley-Barnes K, Sondalle S, Abriola L, Surovtseva Y, Baserga S. Human nucleolar protein 7 (NOL7) is required for early pre-rRNA accumulation and pre-18S rRNA processing. RNA Biology 2023, 20: 257-271. PMID: 37246770, PMCID: PMC10228412, DOI: 10.1080/15476286.2023.2217392.Peer-Reviewed Original ResearchMeSH KeywordsHumansNuclear ProteinsRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, Ribosomal, 18SRNA, Small NucleolarSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsPre-rRNA accumulationRibosome biogenesisNonessential roleEukaryotic ribosome biogenesisEssential cellular processesNucleolar stress responsePre-rRNA levelsRRNA processingLikely orthologCellular processesAssociated proteinsTumor suppressorStress responseHuman cellsProtein synthesisProtein 7Human counterpartBiogenesisYeastOrthologsHomologSubcomplexAccumulationRRNATranscription
2016
Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA
Zhang J, McCann KL, Qiu C, Gonzalez LE, Baserga SJ, Hall TM. Nop9 is a PUF-like protein that prevents premature cleavage to correctly process pre-18S rRNA. Nature Communications 2016, 7: 13085. PMID: 27725644, PMCID: PMC5062617, DOI: 10.1038/ncomms13085.Peer-Reviewed Original ResearchConceptsEukaryotic ribosome biogenesisCorrect subcellular locationRibosome assembly factorsPre-ribosomal RNAPumilio repeatsRibosome biogenesisHuman ribosomopathiesAssembly factorsBiogenesis factorsRepeat proteinsMature rRNASubcellular locationNop9RNA complexCleavage siteRRNATimely cleavageProteinStructural featuresFinal processing stepRibosomopathiesBiogenesisCleavageYeastNucleaseThe molecular basis for ANE syndrome revealed by the large ribosomal subunit processome interactome
McCann KL, Teramoto T, Zhang J, Hall T, Baserga SJ. The molecular basis for ANE syndrome revealed by the large ribosomal subunit processome interactome. ELife 2016, 5: e16381. PMID: 27077951, PMCID: PMC4859800, DOI: 10.7554/elife.16381.Peer-Reviewed Original ResearchMeSH KeywordsAlopeciaCircular DichroismEndocrine System DiseasesHumansIntellectual DisabilityMagnetic Resonance SpectroscopyModels, BiologicalMutant ProteinsProtein BindingProtein FoldingProtein Interaction MapsRibonucleoproteins, Small NucleolarRibosome Subunits, LargeRNA PrecursorsRNA Processing, Post-TranscriptionalRNA-Binding ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsConceptsProtein-protein interactionsANE syndromeMolecular basisDefective protein foldingRRNA processing defectsRNA recognition motifMature ribosomesRibosome assemblyHub proteinsRRNA processingNucleolar proteinsDomain foldingProtein foldingRecognition motifHuman diseasesProcessing defectsInteractomeMutationsCircular dichroismHub functionModel systemYeastFoldingProteinNOP4
2015
A protein interaction map of the LSU processome
McCann KL, Charette JM, Vincent NG, Baserga SJ. A protein interaction map of the LSU processome. Genes & Development 2015, 29: 862-875. PMID: 25877921, PMCID: PMC4403261, DOI: 10.1101/gad.256370.114.Peer-Reviewed Original ResearchMeSH KeywordsProtein Interaction MapsReproducibility of ResultsRibosomal ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsTwo-Hybrid System TechniquesConceptsLarge ribosomal subunitBiogenesis factorsRibosome biogenesis factorsTwo-hybrid assayProtein interaction mapsProtein-protein interactionsSystems biology approachInteractome mapHub proteinsBiology approachProcessomeProtein pairsRibosomal subunitInteraction mapCoordinated processConcerted actionCurrent knowledgeEukaryotesNOP4SubcomplexSubunitsProteinFourfold increaseMaturationAssembly
2000
A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis
Dunbar D, Dragon F, Lee S, Baserga S. A nucleolar protein related to ribosomal protein L7 is required for an early step in large ribosomal subunit biogenesis. Proceedings Of The National Academy Of Sciences Of The United States Of America 2000, 97: 13027-13032. PMID: 11087857, PMCID: PMC27172, DOI: 10.1073/pnas.97.24.13027.Peer-Reviewed Original ResearchConceptsNucleolar proteinsLarge ribosomal subunit biogenesisLarge ribosomal subunit RNARibosomal subunit biogenesisRNA-binding domainRibosomal subunit RNAPulse-chase labeling experimentsProcessing of precursorsRibosomal protein L7Subunit biogenesisExonucleolytic trimmingEssential genesRibosomal proteinsSpecificity factorMature rRNAPrecursor RNASpacer 2L7 proteinSubunit RNAEndonucleolytic cleavageProtein L7Extensive identityRRNAMajor substratePrimer extension
1999
Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins.
Lyman S, Gerace L, Baserga S. Human Nop5/Nop58 is a component common to the box C/D small nucleolar ribonucleoproteins. RNA 1999, 5: 1597-604. PMID: 10606270, PMCID: PMC1369881, DOI: 10.1017/s1355838299991288.Peer-Reviewed Original ResearchAmino Acid SequenceAnimalsCell NucleolusCloning, MolecularConserved SequenceHeLa CellsHumansMiceMolecular Sequence DataMolecular WeightNuclear ProteinsRatsRecombinant ProteinsRibonucleoproteinsRibonucleoproteins, Small NucleolarSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidImp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing
Lee S, Baserga S. Imp3p and Imp4p, Two Specific Components of the U3 Small Nucleolar Ribonucleoprotein That Are Essential for Pre-18S rRNA Processing. Molecular And Cellular Biology 1999, 19: 5441-5452. PMID: 10409734, PMCID: PMC84386, DOI: 10.1128/mcb.19.8.5441.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceGenes, ReporterGenetic TechniquesGenetic VectorsMacromolecular SubstancesMolecular Sequence DataPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRibosomal ProteinsRNA PrecursorsRNA, FungalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentSequence Homology, Amino AcidConceptsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinProtein componentsU3 snoRNPNucleolar ribonucleoproteinGenetic depletionTwo-hybrid methodologyNovel protein componentsPre-rRNA processingUnique protein componentRibosomal proteinsSnoRNA levelsRRNA processingU3 snoRNARRNA precursorIMP proteinU3 RNAS4 familiesPrecise functionSevere defectsCleavage eventsMpp10pProteinSnoRNPsRNA
1997
Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10
Lee S, Baserga S. Functional separation of pre-rRNA processing steps revealed by truncation of the U3 small nucleolar ribonucleoprotein component, Mpp10. Proceedings Of The National Academy Of Sciences Of The United States Of America 1997, 94: 13536-13541. PMID: 9391061, PMCID: PMC28341, DOI: 10.1073/pnas.94.25.13536.Peer-Reviewed Original ResearchMpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast
Dunbar D, Wormsley S, Agentis T, Baserga S. Mpp10p, a U3 Small Nucleolar Ribonucleoprotein Component Required for Pre-18S rRNA Processing in Yeast. Molecular And Cellular Biology 1997, 17: 5803-5812. PMID: 9315638, PMCID: PMC232428, DOI: 10.1128/mcb.17.10.5803.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceAnimalsAntibodiesCloning, MolecularEscherichia coliGenes, FungalHumansMiceMolecular Sequence DataMolecular WeightPhosphoproteinsRecombinant Fusion ProteinsRibonucleoproteinsRibonucleoproteins, Small NuclearRNA PrecursorsRNA Processing, Post-TranscriptionalRNA, RibosomalRNA, Ribosomal, 18SSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidSpores, FungalConceptsYeast proteinsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinNovel protein componentsPre-rRNA processingSmall nucleolar ribonucleoproteinS. cerevisiae cellsSpecific protein componentsPulse-chase analysisMitotic phosphorylationRibosome biogenesisEssential genesMpp10pYeast SaccharomycesNovel proteinU3 snoRNARRNA precursorNucleolar ribonucleoproteinSites A0Tetrad analysisHuman proteinsNull allelesCerevisiae cellsGenBank searchMpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors.
Baserga S, Agentis T, Wormsley S, Dunbar D, Lee S. Mpp10p, a new protein component of the U3 snoRNP required for processing of 18S rRNA precursors. Nucleic Acids Symposium Series 1997, 64-7. PMID: 9478208.Peer-Reviewed Original ResearchConceptsPre-rRNA processingNew protein componentsU3 snoRNPProtein componentsU3 small nucleolar ribonucleoproteinSmall nucleolar ribonucleoproteinEssential genesSlow growthConditional promoterU3 snoRNAMpp10pNucleolar ribonucleoproteinRRNA precursorProcessing eventsYeastSnoRNPsMpp10ProteinModel systemDegrees C. AnalysisC. AnalysisSnoRNAsMutantsRibonucleoproteinSaccharomyces