α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices
Vargas K, Colosi P, Girardi E, Park J, Harmon L, Chandra S. α-Synuclein colocalizes with AP180 and affects the size of clathrin lattices. Journal Of Biological Chemistry 2023, 299: 105091. PMID: 37516240, PMCID: PMC10470054, DOI: 10.1016/j.jbc.2023.105091.Peer-Reviewed Original ResearchConceptsClathrin punctaClathrin assemblyEndocytic accessory proteinsΑ-synucleinPresynaptic membraneSynaptic vesicle cyclingImmuno-electron microscopyClathrin structuresAccessory proteinsClathrin latticesMembrane curvatureVesicle cyclingCell membranePresynaptic proteinsLipid monolayer systemProteinΓ-synucleinMembranePunctaAssemblyRelocalizesClathrinColocalizesVesicle sizeDeletionDopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease
Vidyadhara D, Somayaji M, Wade N, Yücel B, Zhao H, Shashaank N, Ribaudo J, Gupta J, Lam T, Sames D, Greene L, Sulzer D, Chandra S. Dopamine transporter and synaptic vesicle sorting defects underlie auxilin-associated Parkinson’s disease. Cell Reports 2023, 42: 112231. PMID: 36920906, PMCID: PMC10127800, DOI: 10.1016/j.celrep.2023.112231.Peer-Reviewed Original ResearchConceptsParkinson's diseaseMotor deficitsDopamine transporterPathogenesis of PDNigral dopaminergic lossProgressive motor deficitsΑ-synuclein pathologyEarly-onset Parkinson's diseaseDopamine sequestrationDopaminergic vulnerabilityDopaminergic lossKO micePresynaptic sitesKO brainNeurochemical analysisDorsal striatumKnockout miceCardinal featuresReuptake kineticsL-DOPADiseaseSynaptic autophagyFunction mutationsDeficitsMice