2009
Gonadotropin inhibitory hormone inhibits basal forebrain vGluT2‐gonadotropin‐releasing hormone neurons via a direct postsynaptic mechanism
Wu M, Dumalska I, Morozova E, Van Den Pol AN, Alreja M. Gonadotropin inhibitory hormone inhibits basal forebrain vGluT2‐gonadotropin‐releasing hormone neurons via a direct postsynaptic mechanism. The Journal Of Physiology 2009, 587: 1401-1411. PMID: 19204051, PMCID: PMC2678216, DOI: 10.1113/jphysiol.2008.166447.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsFemaleGamma-Aminobutyric AcidGlutamate DecarboxylaseGlutamic AcidGlycoproteinsGonadotropin-Releasing HormoneGreen Fluorescent ProteinsImmunohistochemistryMaleMiceMice, TransgenicNeural InhibitionNeuronsNeuropeptidesPatch-Clamp TechniquesPotassiumProsencephalonRecombinant Fusion ProteinsSynaptic PotentialsSynaptic TransmissionTime FactorsVesicular Glutamate Transport Protein 2ConceptsGnIH/RFRPGonadotropin-inhibitory hormoneHormone neuronsGnRH neuronsGonadotropin releaseRFRP-3Inhibitory hormoneGonadotropin-releasing hormone (GnRH) neuronsAvian gonadotropin-inhibitory hormoneDirect postsynaptic mechanismGAD67-GFP miceVesicular glutamate transportersTarget brain regionsGABAergic neuronsGlutamate releasePostsynaptic mechanismsAnterior pituitaryBrain slicesMedian eminenceGlutamate transportersReproductive functionBrain regionsInhibitory actionElectrophysiological recordingsNeurons
2008
Excitatory Effects of the Puberty-Initiating Peptide Kisspeptin and Group I Metabotropic Glutamate Receptor Agonists Differentiate Two Distinct Subpopulations of Gonadotropin-Releasing Hormone Neurons
Dumalska I, Wu M, Morozova E, Liu R, van den Pol A, Alreja M. Excitatory Effects of the Puberty-Initiating Peptide Kisspeptin and Group I Metabotropic Glutamate Receptor Agonists Differentiate Two Distinct Subpopulations of Gonadotropin-Releasing Hormone Neurons. Journal Of Neuroscience 2008, 28: 8003-8013. PMID: 18685025, PMCID: PMC2597556, DOI: 10.1523/jneurosci.1225-08.2008.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBarium CompoundsCapillariesChloridesDiagonal Band of BrocaDrug ResistanceElectrophysiologyFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsIn Vitro TechniquesMaleMedian EminenceMethoxyhydroxyphenylglycolMiceMice, TransgenicNeuronsPotassium ChannelsRatsRats, Sprague-DawleyReceptors, Metabotropic GlutamateRNA, MessengerSynapsesTumor Suppressor ProteinsVesicular Glutamate Transport Protein 2ConceptsMetabotropic glutamate receptor agonistGlutamate receptor agonistsGroup I metabotropic glutamate receptor agonistReceptor agonistGnRH neuronsGonadotropin-Releasing Hormone NeuronsG protein-coupled receptor GPR54Vesicular glutamate transporter 2GnRH-GFP miceRetrograde labeling techniqueGlutamate transporter 2Gonadotropin-releasing hormoneDistinct subpopulationsOnset of pubertyPostpubertal miceHormone neuronsGnRH cellsExcitatory effectsCentral releaseHypogonadotropic hypogonadismPeptide kisspeptinReceptor GPR54Brain slicesNormal pubertyKisspeptin