2009
Melanin-concentrating hormone directly inhibits GnRH neurons and blocks kisspeptin activation, linking energy balance to reproduction
Wu M, Dumalska I, Morozova E, van den Pol A, Alreja M. Melanin-concentrating hormone directly inhibits GnRH neurons and blocks kisspeptin activation, linking energy balance to reproduction. Proceedings Of The National Academy Of Sciences Of The United States Of America 2009, 106: 17217-17222. PMID: 19805188, PMCID: PMC2761345, DOI: 10.1073/pnas.0908200106.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBrainEnergy MetabolismFemaleGonadotropin-Releasing HormoneGreen Fluorescent ProteinsHypothalamic HormonesImmunohistochemistryKisspeptinsMaleMelaninsMembrane PotentialsMiceMice, TransgenicMicroscopy, FluorescenceNeuronsPatch-Clamp TechniquesPeptide FragmentsPituitary HormonesReceptors, SomatostatinReproductionTime FactorsTumor Suppressor ProteinsVesicular Glutamate Transport Protein 2ConceptsInhibitory effectDirect postsynaptic effectGnRH-GFP neuronsMCH knockout miceMCH-immunoreactive fibersRole of MCHMelanin-concentrating hormone (MCH) systemMCH receptor antagonistsPatch-clamp recordingsMelanin-concentrating hormoneNegative energy balanceKisspeptin activationDrug abuse behaviorGnRH neuronsPostsynaptic effectsExcitatory effectsGABAergic neuronsTransgenic GFP miceImmunoreactive fibersReceptor antagonistCNS neuronsAntidepressant drugsBrain slicesFood restrictionKnockout miceGonadotropin inhibitory hormone inhibits basal forebrain vGluT2‐gonadotropin‐releasing hormone neurons via a direct postsynaptic mechanism
Wu M, Dumalska I, Morozova E, Van Den Pol AN, Alreja M. Gonadotropin inhibitory hormone inhibits basal forebrain vGluT2‐gonadotropin‐releasing hormone neurons via a direct postsynaptic mechanism. The Journal Of Physiology 2009, 587: 1401-1411. PMID: 19204051, PMCID: PMC2678216, DOI: 10.1113/jphysiol.2008.166447.Peer-Reviewed Original ResearchMeSH KeywordsAcetylcholineAnimalsFemaleGamma-Aminobutyric AcidGlutamate DecarboxylaseGlutamic AcidGlycoproteinsGonadotropin-Releasing HormoneGreen Fluorescent ProteinsImmunohistochemistryMaleMiceMice, TransgenicNeural InhibitionNeuronsNeuropeptidesPatch-Clamp TechniquesPotassiumProsencephalonRecombinant Fusion ProteinsSynaptic PotentialsSynaptic TransmissionTime FactorsVesicular Glutamate Transport Protein 2ConceptsGnIH/RFRPGonadotropin-inhibitory hormoneHormone neuronsGnRH neuronsGonadotropin releaseRFRP-3Inhibitory hormoneGonadotropin-releasing hormone (GnRH) neuronsAvian gonadotropin-inhibitory hormoneDirect postsynaptic mechanismGAD67-GFP miceVesicular glutamate transportersTarget brain regionsGABAergic neuronsGlutamate releasePostsynaptic mechanismsAnterior pituitaryBrain slicesMedian eminenceGlutamate transportersReproductive functionBrain regionsInhibitory actionElectrophysiological recordingsNeurons
2006
Muscarine activates the sodium–calcium exchanger via M3 receptors in basal forebrain neurons
Xu C, Wu M, Morozova E, Alreja M. Muscarine activates the sodium–calcium exchanger via M3 receptors in basal forebrain neurons. European Journal Of Neuroscience 2006, 24: 2309-2313. PMID: 17074051, DOI: 10.1111/j.1460-9568.2006.05118.x.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBasal GangliaBiotransformationDiagonal Band of BrocaDiphenylacetic AcidsElectrophysiologyIn Vitro TechniquesIon ChannelsIonsLithium ChlorideMaleMuscarineMuscarinic AgonistsPatch-Clamp TechniquesPiperidinesProsencephalonRatsRats, Sprague-DawleyReceptor, Muscarinic M3Sodium-Calcium ExchangerThioureaConceptsMuscarinic activationWhole-cell patch-clamp recording techniqueMedial septum/diagonal bandPatch-clamp recording techniquesSeptohippocampal GABAergic neuronsBasal forebrain neuronsMuscarinic cholinergic mechanismsRat brain slicesHippocampus-dependent learningHippocampal theta rhythmSodium-calcium exchangerIntraseptal carbacholMSDB neuronsCholinergic neuronsExcitatory actionGABAergic neuronsCholinergic mechanismsKB-R7943M3 receptorsForebrain neuronsDiagonal bandPrimary involvementBrain slicesHippocampal functionPotent modulator