2022
NO Synthesis but Not Apoptosis, Mitosis or Inflammation Can Explain Correlations between Flow Directionality and Paracellular Permeability of Cultured Endothelium
Ghim M, Yang S, David K, Eustaquio J, Warboys C, Weinberg P. NO Synthesis but Not Apoptosis, Mitosis or Inflammation Can Explain Correlations between Flow Directionality and Paracellular Permeability of Cultured Endothelium. International Journal Of Molecular Sciences 2022, 23: 8076. PMID: 35897652, PMCID: PMC9332325, DOI: 10.3390/ijms23158076.Peer-Reviewed Original Research
2021
Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies.
Pang K, Ghim M, Arshad M, Wang X, Weinberg P. Segmenting Growth of Endothelial Cells in 6-Well Plates on an Orbital Shaker for Mechanobiological Studies. Journal Of Visualized Experiments 2021 PMID: 34152321, DOI: 10.3791/61817.Peer-Reviewed Original ResearchEndothelial cells do not align with the mean wall shear stress vector
Arshad M, Ghim M, Mohamied Y, Sherwin S, Weinberg P. Endothelial cells do not align with the mean wall shear stress vector. Journal Of The Royal Society Interface 2021, 18: 20200772. PMID: 33435845, PMCID: PMC7879765, DOI: 10.1098/rsif.2020.0772.Peer-Reviewed Original ResearchMeSH KeywordsAortaAtherosclerosisEndothelial CellsHumansHydrodynamicsShear StrengthStress, Mechanical
2020
The Role of Tricellular Junctions in the Transport of Macromolecules Across Endothelium
Ghim M, Mohamied Y, Weinberg P. The Role of Tricellular Junctions in the Transport of Macromolecules Across Endothelium. Cardiovascular Engineering And Technology 2020, 12: 101-113. PMID: 32820467, PMCID: PMC7904563, DOI: 10.1007/s13239-020-00483-x.Peer-Reviewed Original ResearchConceptsDensity lipoproteinHigh-density lipoproteinLow-density lipoproteinNew therapeutic strategiesEffects of agonistsEndothelial barrier functionConclusionThese dataInfluence of agonistsTherapeutic strategiesVascular endotheliumEndotheliumBarrier functionEndothelial monolayersNormal physiologyTransendothelial transportAgonistsLipoproteinDiseaseFurther investigation
2019
Understanding mechanobiology in cultured endothelium: A review of the orbital shaker method
Warboys C, Ghim M, Weinberg P. Understanding mechanobiology in cultured endothelium: A review of the orbital shaker method. Atherosclerosis 2019, 285: 170-177. PMID: 31096159, PMCID: PMC6570700, DOI: 10.1016/j.atherosclerosis.2019.04.210.Peer-Reviewed Original Research
2017
Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow
Ghim M, Alpresa P, Yang S, Braakman S, Gray S, Sherwin S, van Reeuwijk M, Weinberg P. Visualization of three pathways for macromolecule transport across cultured endothelium and their modification by flow. AJP Heart And Circulatory Physiology 2017, 313: h959-h973. PMID: 28754719, PMCID: PMC5792200, DOI: 10.1152/ajpheart.00218.2017.Peer-Reviewed Original ResearchConceptsShear stressDifferent flow profilesType of flowTransverse stressesNumerical simulationsFlow profileFluid mechanical forcesShear profileShear vectorSolute transportTwo-pore theoryStatic conditionsFlowMacromolecule transportMechanical forcesTransportStressMicrovessel wallShearOrbital shakerStress metricsLong axisTracer distributionSimulations