2023
HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy
Chen M, Menon M, Wang W, Fu J, Yi Z, Sun Z, Liu J, Li Z, Mou L, Banu K, Lee S, Dai Y, Anandakrishnan N, Azeloglu E, Lee K, Zhang W, Das B, He J, Wei C. HCK induces macrophage activation to promote renal inflammation and fibrosis via suppression of autophagy. Nature Communications 2023, 14: 4297. PMID: 37463911, PMCID: PMC10354075, DOI: 10.1038/s41467-023-40086-3.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutophagyFibrosisHumansInflammationKidneyMacrophage ActivationMiceMice, Inbred C57BLNephritisProto-Oncogene Proteins c-hckRenal Insufficiency, ChronicUreteral ObstructionConceptsChronic kidney diseasePro-inflammatory polarizationBone marrow-derived macrophagesRenal inflammationKidney fibrosisMacrophage activationHematopoietic cell kinaseMacrophage pro-inflammatory activityProgressive chronic kidney diseaseIschemia-reperfusion injury modelUnilateral ureteral obstruction kidneysChronic allograft injuryReperfusion injury modelPro-inflammatory activityPro-inflammatory macrophagesHck inhibitorsMarrow-derived macrophagesSuppression of autophagyAllograft injuryUUO miceRenal fibrosisKidney diseaseDiseased kidneysMacrophage numbersInjury model
2018
SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts
Wei C, Banu K, Garzon F, Basgen JM, Philippe N, Yi Z, Liu R, Choudhuri J, Fribourg M, Liu T, Cumpelik A, Wong J, Khan M, Das B, Keung K, Salem F, Campbell KN, Kaufman L, Cravedi P, Zhang W, O'Connell PJ, He JC, Murphy B, Menon MC. SHROOM3-FYN Interaction Regulates Nephrin Phosphorylation and Affects Albuminuria in Allografts. Journal Of The American Society Of Nephrology 2018, 29: 2641-2657. PMID: 30341149, PMCID: PMC6218856, DOI: 10.1681/asn.2018060573.Peer-Reviewed Original ResearchMeSH KeywordsActin CytoskeletonAdolescentAdultAgedAlbuminuriaAllograftsAnimalsChildChild, PreschoolEnhancer Elements, GeneticFemaleGene Knockdown TechniquesGlomerular Filtration RateHomozygoteHumansKidneyKidney TransplantationMaleMembrane ProteinsMiceMice, 129 StrainMice, Inbred C57BLMice, KnockoutMicrofilament ProteinsMiddle AgedPhosphorylationPodocytesPolymorphism, Single NucleotideProto-Oncogene Proteins c-fynRenal Insufficiency, ChronicRNA, Small InterferingSignal TransductionSrc Homology DomainsYoung AdultConceptsDiffuse foot process effacementKnockdown miceFoot process effacementReduced albuminuriaAllograft fibrosisRenal functionDonor kidneysLower GFRRenal fibrosisTGF-b signalingHuman allograftsNephroseq databaseBiopsy samplesProcess effacementProtective roleAlbuminuriaAdult glomeruliHuman podocytesKnockdown podocytesCKDHuman dataPodocytesAllograftsActin cytoskeleton pathwayFibrosis
2016
Biopsy transcriptome expression profiling to identify kidney transplants at risk of chronic injury: a multicentre, prospective study
O'Connell PJ, Zhang W, Menon MC, Yi Z, Schröppel B, Gallon L, Luan Y, Rosales IA, Ge Y, Losic B, Xi C, Woytovich C, Keung KL, Wei C, Greene I, Overbey J, Bagiella E, Najafian N, Samaniego M, Djamali A, Alexander SI, Nankivell BJ, Chapman JR, Smith RN, Colvin R, Murphy B. Biopsy transcriptome expression profiling to identify kidney transplants at risk of chronic injury: a multicentre, prospective study. The Lancet 2016, 388: 983-993. PMID: 27452608, PMCID: PMC5014570, DOI: 10.1016/s0140-6736(16)30826-1.Peer-Reviewed Original ResearchConceptsChronic allograft damage indexAllograft lossKidney transplantPathological variablesChronic injuryEarly allograft lossKidney transplant recipientsRenal allograft recipientsFunction 3 monthsBaseline clinical variablesDevelopment of fibrosisAllograft recipientsNormal allograftsRenal allograftsTransplant recipientsAllograft fibrosisMulticentre studyProgressive injuryProspective studyClinical variablesIndependent cohortLower riskFibrosisPredictive valueTransplant