Featured Publications
A transcriptomic atlas of mammalian olfactory mucosae reveals an evolutionary influence on food odor detection in humans
Saraiva L, Riveros-McKay F, Mezzavilla M, Abou-Moussa E, Arayata C, Makhlouf M, Trimmer C, Ibarra-Soria X, Khan M, Van Gerven L, Jorissen M, Gibbs M, O'Flynn C, McGrane S, Mombaerts P, Marioni J, Mainland J, Logan D. A transcriptomic atlas of mammalian olfactory mucosae reveals an evolutionary influence on food odor detection in humans. Science Advances 2019, 5: eaax0396. PMID: 31392275, PMCID: PMC6669018, DOI: 10.1126/sciadv.aax0396.Peer-Reviewed Original ResearchConceptsOlfactory sensory neuronsChemosensory receptor gene familiesSpecies-specific adaptationsOlfactory sensory neurons subtypesCanonical olfactory receptorsReceptor gene familyOlfactory mucosa samplesMammalian olfactory mucosaGene familyEcological nichesMammalian evolutionTranscriptomic atlasAnalyzed speciesEvolutionary dynamicsRNA sequencingMammalian olfactory systemEvolutionary influencesOlfactory mucosaHomologous subtypesSensory neuronsOlfactory receptorsMucosa samplesOlfactory systemSubtypesMammalian olfactionHierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq
Saraiva L, Ibarra-Soria X, Khan M, Omura M, Scialdone A, Mombaerts P, Marioni J, Logan D. Hierarchical deconstruction of mouse olfactory sensory neurons: from whole mucosa to single-cell RNA-seq. Scientific Reports 2015, 5: 18178. PMID: 26670777, PMCID: PMC4680959, DOI: 10.1038/srep18178.Peer-Reviewed Original ResearchConceptsMature olfactory sensory neuronsOlfactory sensory neuronsRNA-seqOR genesGene expressionSingle-cell RNA-seqIntact OR genesOlfactory receptorsCell typesSingle-nucleotide polymorphismsOR-expressing OSNsOR gene expressionIntact olfactory receptorsNeuron-one receptor ruleCrude tissue samplesMonoallelic expressionNeuronal cell typesMouse olfactory mucosaMultiple cell typesChemosensory tissuesGenesCellular heterogeneityOlfactory mucosaSensory neuronsNon-neuronalA Large-Scale Analysis of Odor Coding in the Olfactory Epithelium
Nara K, Saraiva L, Ye X, Buck L. A Large-Scale Analysis of Odor Coding in the Olfactory Epithelium. Journal Of Neuroscience 2011, 31: 9179-9191. PMID: 21697369, PMCID: PMC3758579, DOI: 10.1523/jneurosci.1282-11.2011.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsFemaleMiceMice, Inbred C57BLOdorantsOlfactory MucosaOlfactory Receptor NeuronsSmellConceptsOlfactory sensory neuronsOdor qualityOdor recognitionMouse olfactory sensory neuronsOdorant receptorsEncode odor identityExpressed odorant receptorOdor identityOdor codingOlfactory epitheliumSensory neuronsPerceived odorMultiple odorantsSensory inputInnate behaviorsOdorLarge-scale analysisOR genesMiceCombinatorial codeA novel olfactory receptor gene family in teleost fish
Saraiva L, Korsching S. A novel olfactory receptor gene family in teleost fish. Genome Research 2007, 17: 1448-1457. PMID: 17717047, PMCID: PMC1987349, DOI: 10.1101/gr.6553207.Peer-Reviewed Original ResearchConceptsOra genesGene pairsMulti-exon structureSpecies-specific expansionOlfactory receptor gene familyV1R-like genesOlfactory receptor familyReceptor gene familySpeciation eventsTeleost lineagePhylogenetic treePhylogenetic analysisGene familyGene regulationGene repertoireGene functionOlfactory epithelium of zebrafishTeleost familiesTeleost speciesOrthologsTeleost fishGenesNegative selectionTeleostFish species
2015
Molecular and neuronal homology between the olfactory systems of zebrafish and mouse
Saraiva L, Ahuja G, Ivandic I, Syed A, Marioni J, Korsching S, Logan D. Molecular and neuronal homology between the olfactory systems of zebrafish and mouse. Scientific Reports 2015, 5: 11487. PMID: 26108469, PMCID: PMC4480006, DOI: 10.1038/srep11487.Peer-Reviewed Original ResearchConceptsDegree of molecular conservationYears of evolutionary divergenceVomeronasal organOlfactory mucosaOlfactory organChemosensory receptor genesOlfactory systemTranscriptome of miceDual olfactory systemEvolutionary divergenceMolecular conservationNeuronal homologRepertoire sizeRNA abundanceMolecular relationshipsCell-specific markersClasses of neuronsMolecular basisChemosensory receptorsMarker genesOrgans of rodentsAbsolute abundanceZebrafishReceptor geneVertebrates
2014
Olfactory Receptor Patterning in a Higher Primate
Horowitz L, Saraiva L, Kuang D, Yoon K, Buck L. Olfactory Receptor Patterning in a Higher Primate. Journal Of Neuroscience 2014, 34: 12241-12252. PMID: 25209267, PMCID: PMC4160765, DOI: 10.1523/jneurosci.1779-14.2014.Peer-Reviewed Original ResearchConceptsTrace amine-associated receptorsHuman trace amine-associated receptorDetect social cuesHuman olfactory perceptionOdorant receptorsMammalian olfactory systemOrganization of sensory inputsSocial cuesOlfactory perceptionOlfactory bulbOlfactory systemSensory inputInstinctive behaviorMouse nosePrimatesDifferent domainsOrganizational strategiesReceptor patternsMacaquesReceptorsCuesChemosensory receptorsMiceOlfactionBrainThe Olfactory Transcriptomes of Mice
Ibarra-Soria X, Levitin M, Saraiva L, Logan D. The Olfactory Transcriptomes of Mice. PLOS Genetics 2014, 10: e1004593. PMID: 25187969, PMCID: PMC4154679, DOI: 10.1371/journal.pgen.1004593.Peer-Reviewed Original ResearchConceptsVomeronasal receptorsVR genesProtein-coding genesProtein coding sequencesDeep RNA sequencingDistribution of abundanceEvidence of expressionCell-specific expressionReference genomeGene annotationMultiple malesMulti-exonQuantitative catalogOlfactory tissueMouse genomeCoding sequenceOR genesOlfactory-mediated behaviorsGenomic techniquesExpression microarraysRNA sequencingGene expressionGenesQuantitative RT-PCRSexual dimorphism