2024
Spartin-mediated lipid transfer facilitates lipid droplet turnover
Wan N, Hong Z, Parson M, Korfhage J, Burke J, Melia T, Reinisch K. Spartin-mediated lipid transfer facilitates lipid droplet turnover. Proceedings Of The National Academy Of Sciences Of The United States Of America 2024, 121: e2314093121. PMID: 38190532, PMCID: PMC10801920, DOI: 10.1073/pnas.2314093121.Peer-Reviewed Original Research
2015
The Legionella Anti-autophagy Effector RavZ Targets the Autophagosome via PI3P- and Curvature-Sensing Motifs
Horenkamp FA, Kauffman KJ, Kohler LJ, Sherwood RK, Krueger KP, Shteyn V, Roy CR, Melia TJ, Reinisch KM. The Legionella Anti-autophagy Effector RavZ Targets the Autophagosome via PI3P- and Curvature-Sensing Motifs. Developmental Cell 2015, 34: 569-576. PMID: 26343456, PMCID: PMC4594837, DOI: 10.1016/j.devcel.2015.08.010.Peer-Reviewed Original ResearchConceptsATG8 proteinsIntracellular pathogen Legionella pneumophilaPre-autophagosomal structureAtg8/LC3 proteinsPathogen Legionella pneumophilaHigh-curvature membranesMembrane transport pathwaysCytosol of cellsEffector proteinsCatalytic domainHost cytosolRavZAutophagy proteinsLC3 proteinPathogenic microbesSubstrate affinityProteinIntermediate membraneLegionella pneumophilaAutophagosomesAutophagyCytosolTransport pathwaysInterfacial activationMembrane
2013
The EM structure of the TRAPPIII complex leads to the identification of a requirement for COPII vesicles on the macroautophagy pathway
Tan D, Cai Y, Wang J, Zhang J, Menon S, Chou HT, Ferro-Novick S, Reinisch KM, Walz T. The EM structure of the TRAPPIII complex leads to the identification of a requirement for COPII vesicles on the macroautophagy pathway. Proceedings Of The National Academy Of Sciences Of The United States Of America 2013, 110: 19432-19437. PMID: 24218626, PMCID: PMC3845172, DOI: 10.1073/pnas.1316356110.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsAutophagyChlorocebus aethiopsChromatography, GelCloning, MolecularCOP-Coated VesiclesCOS CellsElectroporationEscherichia coliImage Processing, Computer-AssistedMicroscopy, ElectronMicroscopy, FluorescenceModels, MolecularProtein ConformationSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsVesicular Transport ProteinsConceptsPhagophore assembly siteCOPII vesiclesAssembly sitesRab GTPase Ypt1Electron microscopy structureTargeting of ERTRAPPIII complexFusion machineryMicroscopy structureCOPII coatMacroautophagy pathwayExchange factorSubunit associatesMembrane sourceEM structuresAcceptor compartmentTRAPPIIITRAPPIVesiclesMacroautophagyTrs85COPIISec23Ypt1Mammals