2023
Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification
Santos-Sacchi J, Bai J, Navaratnam D. Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification. Journal Of Neuroscience 2023, 43: 2460-2468. PMID: 36868859, PMCID: PMC10082455, DOI: 10.1523/jneurosci.2033-22.2023.Peer-Reviewed Original ResearchConceptsConformational switchingMammalian hearingMembrane motor proteinCochlear amplificationMotor protein prestinVoltage-sensor charge movementOuter hair cell electromotilityHair cell electromotilityProtein prestinMotor proteinsConformation switchingCharge movementPrestinPrestin activityCell membraneOHC electromotilityHair cell membraneElectromotilityTransmembrane voltageOrgan of CortiAmplificationMammalsMembrane capacitanceProtein
2011
The magnificent outer hair cell of Corti's organ
Santos-Sacchi J. The magnificent outer hair cell of Corti's organ. Otology Japan 2011, 9: 111-115. DOI: 10.11289/otoljpn1991.9.111.Peer-Reviewed Original Research
2001
Furosemide alters nonlinear capacitance in isolated outer hair cells
Santos-Sacchi J, Wu M, Kakehata S. Furosemide alters nonlinear capacitance in isolated outer hair cells. Hearing Research 2001, 159: 69-73. PMID: 11520635, DOI: 10.1016/s0378-5955(01)00321-5.Peer-Reviewed Original ResearchConceptsOuter hair cellsWhole-cell patch-clamp techniqueHair cellsNormal cochlear functionIsolated outer hair cellsPatch-clamp techniqueOrgan of CortiLoop diureticsSensory dysfunctionHearing resultsCochlear functionEndolymphatic potentialFurosemideDirect effectHill coefficientPositive levelCellsDiureticsDysfunctionPresent results
2000
Voltage Gating of Gap Junctions in Cochlear Supporting Cells: Evidence for Nonhomotypic Channels
Zhao H, Santos-Sacchi J. Voltage Gating of Gap Junctions in Cochlear Supporting Cells: Evidence for Nonhomotypic Channels. The Journal Of Membrane Biology 2000, 175: 17-24. PMID: 10811964, DOI: 10.1007/s002320001051.Peer-Reviewed Original Research
1994
Cell coupling in the supporting cells of Corti's organ: Sensitivity to intracellular H+ and Ca+2
Sato Y, Santos-Sacchi J. Cell coupling in the supporting cells of Corti's organ: Sensitivity to intracellular H+ and Ca+2. Hearing Research 1994, 80: 21-24. PMID: 7852199, DOI: 10.1016/0378-5955(94)90004-3.Peer-Reviewed Original Research
1991
Isolated supporting cells from the organ of Corti: Some whole cell electrical characteristics and estimates of gap junctional conductance
Santos-Sacchi J. Isolated supporting cells from the organ of Corti: Some whole cell electrical characteristics and estimates of gap junctional conductance. Hearing Research 1991, 52: 89-98. PMID: 2061216, DOI: 10.1016/0378-5955(91)90190-k.Peer-Reviewed Original ResearchConceptsInput resistanceWhole-cell voltage clamp studiesAverage input resistanceHensen's cellsOrgan of CortiVoltage-clamp studiesGuinea pig organJunctional resistanceClamp studiesCell levelPig organsSingle-cell levelGap junctional conductanceG omegaElectrical activityExternal TEACell couplingCortiJunctional conductanceCellsOrgansSensitive indicatorSmall groupCell values
1989
Asymmetry in voltage-dependent movements of isolated outer hair cells from the organ of Corti
Santos-Sacchi J. Asymmetry in voltage-dependent movements of isolated outer hair cells from the organ of Corti. Journal Of Neuroscience 1989, 9: 2954-2962. PMID: 2769373, PMCID: PMC6569687, DOI: 10.1523/jneurosci.09-08-02954.1989.Peer-Reviewed Original ResearchConceptsOuter hair cellsWhole-cell voltage-clamp techniqueHair cellsNormal resting potentialsOrgan of CortiVoltage-clamp techniqueCell shorteningAcoustic stimulationReceptor potentialResting potentialsResponse magnitudeOHC movementsReported productionStimulationHyperpolarizationComparable levelsNeural tuningCell length changesDepolarizationLength changesCellsBasilar membraneVoltage stimulation
1987
Electrical coupling differs in the in vitro and in vivo organ of Corti
Santos-Sacchi J. Electrical coupling differs in the in vitro and in vivo organ of Corti. Hearing Research 1987, 25: 227-232. PMID: 3558131, DOI: 10.1016/0378-5955(87)90094-3.Peer-Reviewed Original ResearchConceptsOrgan of CortiBony cochleaVivo preparationInner earLateral wallGuinea pig organAnesthetized animalsPig organsStria vascularisEndolymphatic potentialCortiReissner's membraneMedium 199Perfusion chamberCochleaVivo experimentsApical sideOrgansKCl electrodesM KCl electrodesElectrical couplingUnique microenvironmentCellsEarThird turn
1986
Dye coupling in the organ of Corti
Santos-Sacchi J. Dye coupling in the organ of Corti. Cell And Tissue Research 1986, 245: 525-529. PMID: 3757014, PMCID: PMC6042516, DOI: 10.1007/bf00218553.Peer-Reviewed Original ResearchThe temperature dependence of electrical coupling in the organ of Corti
Santos-Sacchi J. The temperature dependence of electrical coupling in the organ of Corti. Hearing Research 1986, 21: 205-211. PMID: 2424883, DOI: 10.1016/0378-5955(86)90219-4.Peer-Reviewed Original Research
1985
The effects of cytoplasmic acidification upon electrical coupling in the organ of Corti
Santos-Sacchi J. The effects of cytoplasmic acidification upon electrical coupling in the organ of Corti. Hearing Research 1985, 19: 207-215. PMID: 3934121, DOI: 10.1016/0378-5955(85)90140-6.Peer-Reviewed Original Research
1984
A re-evaluation of cell coupling in the organ of Corti
Santos-Sacchi J. A re-evaluation of cell coupling in the organ of Corti. Hearing Research 1984, 14: 203-204. PMID: 6746433, DOI: 10.1016/0378-5955(84)90019-4.Peer-Reviewed Original Research
1983
Intercellular communication in the supporting cells of the organ of Corti
Santos-Sacchi J, Dallos P. Intercellular communication in the supporting cells of the organ of Corti. Hearing Research 1983, 9: 317-326. PMID: 6841286, DOI: 10.1016/0378-5955(83)90034-5.Peer-Reviewed Original Research
1982
Determination of cochlear receptor potential characteristics by pseudorandom noise
Dallos P, Santos-Sacchi J, Lutz W. Determination of cochlear receptor potential characteristics by pseudorandom noise. The Journal Of The Acoustical Society Of America 1982, 71: s17-s17. DOI: 10.1121/1.2019254.Peer-Reviewed Original Research