2023
Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification
Santos-Sacchi J, Bai J, Navaratnam D. Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification. Journal Of Neuroscience 2023, 43: 2460-2468. PMID: 36868859, PMCID: PMC10082455, DOI: 10.1523/jneurosci.2033-22.2023.Peer-Reviewed Original ResearchConceptsConformational switchingMammalian hearingMembrane motor proteinCochlear amplificationMotor protein prestinVoltage-sensor charge movementOuter hair cell electromotilityHair cell electromotilityProtein prestinMotor proteinsConformation switchingCharge movementPrestinPrestin activityCell membraneOHC electromotilityHair cell membraneElectromotilityTransmembrane voltageOrgan of CortiAmplificationMammalsMembrane capacitanceProtein
2022
Single particle cryo-EM structure of the outer hair cell motor protein prestin
Butan C, Song Q, Bai JP, Tan WJT, Navaratnam D, Santos-Sacchi J. Single particle cryo-EM structure of the outer hair cell motor protein prestin. Nature Communications 2022, 13: 290. PMID: 35022426, PMCID: PMC8755724, DOI: 10.1038/s41467-021-27915-z.Peer-Reviewed Original ResearchConceptsTransmembrane domainProtein prestinSingle-particle cryo-EM structuresAnti-sigma factor antagonist domainOuter hair cell motor protein prestinCryo-EM structureCryo-electron microscopyMotor protein prestinSLC26 family membersSulfate transportersTransmembrane segmentsPrestin functionÅ resolutionPrestinOHC electromotilityOpen stateCochlear amplificationPutative mechanismsFamily membersDomainRepeatsSLC26A9TransportersMutationsElectromotility
2011
Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ
Song L, Santos-Sacchi J, Shera C, Olson E. Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ. AIP Conference Proceedings 2011, 1403: 179-184. DOI: 10.1063/1.3658083.Peer-Reviewed Original ResearchCellular mechanicsConformational changesMotor protein prestinProtein prestinMolecular eventsLateral membranesOHC lateral membraneCellular expressionWhole cellsElectromotilityOuter Hair CellsPrestinHair cellsReceptor potentialNonlinear capacitanceMolecular conformational changesIntracellular chloride levelsCellsTight couplingOrgansOHC receptor potentialsExpressionMembraneNormal levelsVivo
2008
Anion Control of Voltage Sensing by the Motor Protein Prestin in Outer Hair Cells
Rybalchenko V, Santos-Sacchi J. Anion Control of Voltage Sensing by the Motor Protein Prestin in Outer Hair Cells. Biophysical Journal 2008, 95: 4439-4447. PMID: 18658219, PMCID: PMC2567960, DOI: 10.1529/biophysj.108.134197.Peer-Reviewed Original ResearchConceptsOuter hair cellsHair cellsCochlear amplificationSLC26 anion transporter familyMotor protein prestinMammalian cochlear amplificationVoltage-sensor charge movementAuditory responsivenessCorti's organAnion transporter familyTransporter familyProtein prestinMolecular motorsCharge movementPrestinOrgansCellsComplicated roleVoltage sensing
2003
Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility
Santos-Sacchi J, Zhao HB. Excitation of fluorescent dyes inactivates the outer hair cell integral membrane motor protein prestin and betrays its lateral mobility. Pflügers Archiv - European Journal Of Physiology 2003, 446: 617-622. PMID: 12783229, DOI: 10.1007/s00424-003-1053-8.Peer-Reviewed Original Research