2023
Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification
Santos-Sacchi J, Bai J, Navaratnam D. Megahertz Sampling of Prestin (SLC26a5) Voltage-Sensor Charge Movements in Outer Hair Cell Membranes Reveals Ultrasonic Activity that May Support Electromotility and Cochlear Amplification. Journal Of Neuroscience 2023, 43: 2460-2468. PMID: 36868859, PMCID: PMC10082455, DOI: 10.1523/jneurosci.2033-22.2023.Peer-Reviewed Original ResearchConceptsConformational switchingMammalian hearingMembrane motor proteinCochlear amplificationMotor protein prestinVoltage-sensor charge movementOuter hair cell electromotilityHair cell electromotilityProtein prestinMotor proteinsConformation switchingCharge movementPrestinPrestin activityCell membraneOHC electromotilityHair cell membraneElectromotilityTransmembrane voltageOrgan of CortiAmplificationMammalsMembrane capacitanceProtein
2022
Single particle cryo-EM structure of the outer hair cell motor protein prestin
Butan C, Song Q, Bai JP, Tan WJT, Navaratnam D, Santos-Sacchi J. Single particle cryo-EM structure of the outer hair cell motor protein prestin. Nature Communications 2022, 13: 290. PMID: 35022426, PMCID: PMC8755724, DOI: 10.1038/s41467-021-27915-z.Peer-Reviewed Original ResearchConceptsTransmembrane domainProtein prestinSingle-particle cryo-EM structuresAnti-sigma factor antagonist domainOuter hair cell motor protein prestinCryo-EM structureCryo-electron microscopyMotor protein prestinSLC26 family membersSulfate transportersTransmembrane segmentsPrestin functionÅ resolutionPrestinOHC electromotilityOpen stateCochlear amplificationPutative mechanismsFamily membersDomainRepeatsSLC26A9TransportersMutationsElectromotility
2020
Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion
Zhai F, Song L, Bai JP, Dai C, Navaratnam D, Santos-Sacchi J. Maturation of Voltage-induced Shifts in SLC26a5 (Prestin) Operating Point during Trafficking and Membrane Insertion. Neuroscience 2020, 431: 128-133. PMID: 32061780, PMCID: PMC8720582, DOI: 10.1016/j.neuroscience.2020.02.003.Peer-Reviewed Original ResearchConceptsMembrane motor proteinVoltage-dependent proteinsMembrane insertionOuter hair cellsMotor proteinsPrestin densityHEK cell linesConformational changesBiophysical forcesOHC electromotilityProteinCell linesMembrane potentialCooperative interactionsPrestinHair cellsCochlear amplificationTransmembrane voltageNonlinear capacitanceMammalsTraffickingVoltage-induced shiftElectromotilityMaturationMembrane
2011
Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ
Song L, Santos-Sacchi J, Shera C, Olson E. Chloride Dependent Coupling of Molecular to Cellular Mechanics in the Outer Hair Cell of Corti's Organ. AIP Conference Proceedings 2011, 1403: 179-184. DOI: 10.1063/1.3658083.Peer-Reviewed Original ResearchCellular mechanicsConformational changesMotor protein prestinProtein prestinMolecular eventsLateral membranesOHC lateral membraneCellular expressionWhole cellsElectromotilityOuter Hair CellsPrestinHair cellsReceptor potentialNonlinear capacitanceMolecular conformational changesIntracellular chloride levelsCellsTight couplingOrgansOHC receptor potentialsExpressionMembraneNormal levelsVivo
2010
Effect of capsaicin on potassium conductance and electromotility of the guinea pig outer hair cell
Wu T, Song L, Shi X, Jiang Z, Santos-Sacchi J, Nuttall A. Effect of capsaicin on potassium conductance and electromotility of the guinea pig outer hair cell. Hearing Research 2010, 272: 117-124. PMID: 21044673, PMCID: PMC3387680, DOI: 10.1016/j.heares.2010.10.010.Peer-Reviewed Original ResearchConceptsOuter hair cellsTRPV-1 channelsHair cellsPrimary sensory neuronsEffects of capsaicinGuinea pig outer hair cellsSensory neuronsPharmacological actionsCapsaicinPotassium conductancePotent blockerPharmacological propertiesDirect actionNovel actionCochlear amplificationK conductanceAuditory effectsClassic activatorAuditory receptionCellsNociceptionElectromotilityBlockersNew findingsNeurons
2005
On the Effect of Prestin on the Electrical Breakdown of Cell Membranes
Navarrete EG, Santos-Sacchi J. On the Effect of Prestin on the Electrical Breakdown of Cell Membranes. Biophysical Journal 2005, 90: 967-974. PMID: 16299081, PMCID: PMC1367121, DOI: 10.1529/biophysj.105.064105.Peer-Reviewed Original ResearchConceptsTSA cellsWhole cell levelVoltage-dependent activityGuinea pig OHCsOuter hair cell motor proteinMotor proteinsPM domainsMaximum transmembrane potentialPrestin activityMembrane susceptibilityTransmembrane potentialCell membranePrestinTsA201 cellsBiophysical influencesMembrane stiffnessElectroporationCellsBasal portionSignificant resistanceMembrane capacitanceProteinElectromotilityAuditory sensitivitySusceptibility
2003
New tunes from Corti’s organ: the outer hair cell boogie rules
Santos-Sacchi J. New tunes from Corti’s organ: the outer hair cell boogie rules. Current Opinion In Neurobiology 2003, 13: 459-468. PMID: 12965294, DOI: 10.1016/s0959-4388(03)00100-4.Peer-Reviewed Original ResearchConceptsSpecial cell typesHair cellsOuter hair cell electromotilityHair cell electromotilityMolecular identificationCell typesMammalsMechanism of actionOuter hair cellsAdditional mechanismVertebratesCellsAmplificationMechanismKnockoutElectromotilityOrgansSuch amplificationCorti's organAcoustic stimuliRemarkable progress
2001
Non-uniform mapping of stress-induced, motility-related charge movement in the outer hair cell plasma membrane
Takahashi S, Santos-Sacchi J. Non-uniform mapping of stress-induced, motility-related charge movement in the outer hair cell plasma membrane. Pflügers Archiv - European Journal Of Physiology 2001, 441: 506-513. PMID: 11212214, DOI: 10.1007/s004240000455.Peer-Reviewed Original ResearchConceptsCell plasma membranePlasma membraneLateral membranesOuter hair cell plasma membraneVoltage-dependent motilityMammalian cochlear amplifierOuter hair cell electromotilityMembrane microdomainsGating currentsHair cell electromotilityTurgor pressureOHC motorMolecular motorsElectromotile responseMembraneMotilityCytoskeletonCharge movementBell-shaped distributionMicrodomainsElectromotilityNon-uniform mappingPronase treatmentCochlear amplifierPronase