2018
Genetically encoded fluorescent voltage indicators: are we there yet?
Platisa J, Pieribone VA. Genetically encoded fluorescent voltage indicators: are we there yet? Current Opinion In Neurobiology 2018, 50: 146-153. PMID: 29501950, PMCID: PMC5984684, DOI: 10.1016/j.conb.2018.02.006.Peer-Reviewed Original Research
2017
Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging
2016
A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices
Abdelfattah AS, Farhi SL, Zhao Y, Brinks D, Zou P, Ruangkittisakul A, Platisa J, Pieribone VA, Ballanyi K, Cohen AE, Campbell RE. A Bright and Fast Red Fluorescent Protein Voltage Indicator That Reports Neuronal Activity in Organotypic Brain Slices. Journal Of Neuroscience 2016, 36: 2458-2472. PMID: 26911693, PMCID: PMC4764664, DOI: 10.1523/jneurosci.3484-15.2016.Peer-Reviewed Original ResearchConceptsVoltage indicatorsBlue-shifted channelrhodopsinRed-shifted fluorescent proteinsFluorescent voltage indicatorsWide-field fluorescence microscopyBlue excitationOptical imagingOptical electrophysiologyLow phototoxicityAutofluorescent backgroundLight photoactivationSingle-trial recordingsTemporal resolutionChannelrhodopsinIntrinsic advantagesExcitationVoltage oscillationsFluorescence microscopyOscillationsGreen indicatorsChromophoreMicroscopyResolution
2015
Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment
Gruber DF, Gaffney JP, Mehr S, DeSalle R, Sparks JS, Platisa J, Pieribone VA. Adaptive Evolution of Eel Fluorescent Proteins from Fatty Acid Binding Proteins Produces Bright Fluorescence in the Marine Environment. PLOS ONE 2015, 10: e0140972. PMID: 26561348, PMCID: PMC4641735, DOI: 10.1371/journal.pone.0140972.Peer-Reviewed Original ResearchConceptsAcid-binding proteinFluorescent proteinFatty acid-binding proteinDuplication eventsGene duplication eventsStrong positive selectionNew fluorescent proteinsMarine environmentAdaptive evolutionEvolutionary switchCryptic genusVertebrate ordersVertebrate taxaPhylogenetic reconstructionProtein familyPhylogenetic analysisSequence motifsSister groupEvolutionary adaptationTranscriptomic analysisPositive selectionFatty acid binding proteinAcid binding proteinBinding proteinBright green fluorescence
2013
Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics
Han Z, Jin L, Platisa J, Cohen LB, Baker BJ, Pieribone VA. Fluorescent Protein Voltage Probes Derived from ArcLight that Respond to Membrane Voltage Changes with Fast Kinetics. PLOS ONE 2013, 8: e81295. PMID: 24312287, PMCID: PMC3842285, DOI: 10.1371/journal.pone.0081295.Peer-Reviewed Original Research
2012
Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals
Osman A, Park JH, Dickensheets D, Platisa J, Culurciello E, Pieribone VA. Design Constraints for Mobile, High-Speed Fluorescence Brain Imaging in Awake Animals. IEEE Transactions On Biomedical Circuits And Systems 2012, 6: 446-453. PMID: 23853231, DOI: 10.1109/tbcas.2012.2226174.Peer-Reviewed Original ResearchA Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials
Barnett L, Platisa J, Popovic M, Pieribone VA, Hughes T. A Fluorescent, Genetically-Encoded Voltage Probe Capable of Resolving Action Potentials. PLOS ONE 2012, 7: e43454. PMID: 22970127, PMCID: PMC3435330, DOI: 10.1371/journal.pone.0043454.Peer-Reviewed Original ResearchSingle Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe
Jin L, Han Z, Platisa J, Wooltorton JR, Cohen LB, Pieribone VA. Single Action Potentials and Subthreshold Electrical Events Imaged in Neurons with a Fluorescent Protein Voltage Probe. Neuron 2012, 75: 779-785. PMID: 22958819, PMCID: PMC3439164, DOI: 10.1016/j.neuron.2012.06.040.Peer-Reviewed Original ResearchGenetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics
Baker BJ, Jin L, Han Z, Cohen LB, Popovic M, Platisa J, Pieribone V. Genetically encoded fluorescent voltage sensors using the voltage-sensing domain of Nematostella and Danio phosphatases exhibit fast kinetics. Journal Of Neuroscience Methods 2012, 208: 190-196. PMID: 22634212, PMCID: PMC3398169, DOI: 10.1016/j.jneumeth.2012.05.016.Peer-Reviewed Original Research