Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight
Platisa J, Vasan G, Yang A, Pieribone VA. Directed Evolution of Key Residues in Fluorescent Protein Inverses the Polarity of Voltage Sensitivity in the Genetically Encoded Indicator ArcLight. ACS Chemical Neuroscience 2017, 8: 513-523. PMID: 28045247, PMCID: PMC5355904, DOI: 10.1021/acschemneuro.6b00234.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCells, CulturedCerebral CortexElectric StimulationEmbryo, MammalianGreen Fluorescent ProteinsHEK293 CellsHumansLuminescent ProteinsMiceModels, MolecularMolecular BiologyMutagenesis, Site-DirectedMutationNeuronsPatch-Clamp TechniquesRecombinant Fusion ProteinsTransfectionVoltage-Sensitive Dye Imaging