2024
Interleukin-7-based identification of liver lymphatic endothelial cells reveals their unique structural features
Yang Y, Jeong J, Su T, Lai S, Zhang P, Garcia-Milian R, Graham M, Liu X, McConnell M, Utsumi T, Pereira J, Iwakiri Y. Interleukin-7-based identification of liver lymphatic endothelial cells reveals their unique structural features. JHEP Reports 2024, 6: 101069. PMID: 38966234, PMCID: PMC11222939, DOI: 10.1016/j.jhepr.2024.101069.Peer-Reviewed Original ResearchCell surface structuresLymphatic endothelial cellsPublished single-cell RNA-sequencingRNA-seq analysisScRNA-seq analysisSingle-cell RNA sequencingLymphatic systemEndothelial cellsInterleukin-7RNA-seqScRNA-seqExpressed genesRNA sequencingTranscriptomic changesLow abundanceI/R liver injuryGenesIsolation protocolLiver cell typesCell typesIsolation methodLiver of miceHuman liver specimensHeterozygous miceMouse liver
2023
The Sympathetic Nervous System Promotes Hepatic Lymphangiogenesis, which Is Protective Against Liver Fibrosis
Tanaka M, Jeong J, Thomas C, Zhang X, Zhang P, Saruwatari J, Kondo R, McConnell M, Utsumi T, Iwakiri Y. The Sympathetic Nervous System Promotes Hepatic Lymphangiogenesis, which Is Protective Against Liver Fibrosis. American Journal Of Pathology 2023, 193: 2182-2202. PMID: 37673329, PMCID: PMC10699132, DOI: 10.1016/j.ajpath.2023.08.004.Peer-Reviewed Original ResearchConceptsPartial portal vein ligationNoncirrhotic portal hypertensionCirrhotic patientsVascular endothelial growth factorLiver fibrosisEndothelial growth factorPortal hypertensionSympathetic denervationSympathetic nervesBDL ratsVascular diseaseIdiopathic noncirrhotic portal hypertensionGrowth factorPortal hypertensive patientsPortal vein ligationSympathetic nervous systemMechanisms of lymphangiogenesisCeliac ganglionectomyHypertensive patientsLymphatic vessel numberLiver biopsyLiver cirrhosisVein ligationPPVL ratsHepatic lymphatic vesselsAn optimized visualization and quantitative protocol for in-depth evaluation of lymphatic vessel architecture in the liver
Jeong J, Tanaka M, Yang Y, Arefyev N, DiRito J, Tietjen G, Zhang X, McConnell M, Utsumi T, Iwakiri Y. An optimized visualization and quantitative protocol for in-depth evaluation of lymphatic vessel architecture in the liver. AJP Gastrointestinal And Liver Physiology 2023, 325: g379-g390. PMID: 37605828, PMCID: PMC10887843, DOI: 10.1152/ajpgi.00139.2023.Peer-Reviewed Original Research
2022
Hepatic lymphatic vascular system in health and disease
Jeong J, Tanaka M, Iwakiri Y. Hepatic lymphatic vascular system in health and disease. Journal Of Hepatology 2022, 77: 206-218. PMID: 35157960, PMCID: PMC9870070, DOI: 10.1016/j.jhep.2022.01.025.Peer-Reviewed Original ResearchConceptsLiver diseaseNon-alcoholic fatty liver diseaseLymphatic systemFatty liver diseaseCongenital liver diseasesPotential therapeutic strategyHepatic lymphatic systemLiver transplantationPortal hypertensionMalignant tumorsTherapeutic strategiesDisease pathogenesisHepatic physiologyDiseasePathological conditionsSpecific markersLymphatic vesselsVascular systemLymphatic vascular systemOrgansTissue homeostasisHypertensionTransplantationPathophysiologyPathogenesis
2021
JAZF1 heterozygous knockout mice show altered adipose development and metabolism
Jeong J, Jang S, Park S, Kwon W, Kim S, Jang S, Ko J, Park S, Lim S, Yoon D, Yi J, Lee S, Kim M, Choi S, Ryoo Z. JAZF1 heterozygous knockout mice show altered adipose development and metabolism. Cell & Bioscience 2021, 11: 161. PMID: 34407873, PMCID: PMC8375039, DOI: 10.1186/s13578-021-00625-1.Peer-Reviewed Original ResearchHigh-fat dietAdipocyte differentiationNormal dietMetabolic disordersType 2 diabetes mellitusAdipose developmentImpaired adipocyte differentiationImpaired glucose homeostasisBody fat massAdipose tissue massHeterozygous knockout miceRegulation of PPARγKnockout mice showDiabetes mellitusControl miceFat massMouse embryonic fibroblastsGlucose homeostasisKnockout miceConclusionOur findingsTissue massAdipose tissueMice showJAZF1 expressionVivo modelJazf1 acts as a regulator of insulin‐producing β‐cell differentiation in induced pluripotent stem cells and glucose homeostasis in mice
Park S, Kwon W, Park S, Jeong J, Kim D, Jang S, Kim S, Sung Y, Kim M, Choi S, Ryoo Z. Jazf1 acts as a regulator of insulin‐producing β‐cell differentiation in induced pluripotent stem cells and glucose homeostasis in mice. The FEBS Journal 2021, 288: 4412-4427. PMID: 33555104, DOI: 10.1111/febs.15751.Peer-Reviewed Original ResearchAlcohol-induced Hsp90 acetylation is a novel driver of liver sinusoidal endothelial dysfunction and alcohol-related liver disease
Yang Y, Sangwung P, Kondo R, Jung Y, McConnell MJ, Jeong J, Utsumi T, Sessa WC, Iwakiri Y. Alcohol-induced Hsp90 acetylation is a novel driver of liver sinusoidal endothelial dysfunction and alcohol-related liver disease. Journal Of Hepatology 2021, 75: 377-386. PMID: 33675874, PMCID: PMC8292196, DOI: 10.1016/j.jhep.2021.02.028.Peer-Reviewed Original ResearchConceptsEndothelial nitric oxide synthaseAlcohol-induced liver injuryLiver sinusoidal endothelial cellsAlcohol-related liver diseaseLiver injuryLSEC dysfunctionHsp90 acetylationNO productionHistone deacetylase 6Liver diseaseTherapeutic strategiesHeat shock protein 90 (Hsp90) acetylationLiver sinusoidal endothelial dysfunctionSinusoidal endothelial cell dysfunctionMouse liver sinusoidal endothelial cellsEndothelial cell dysfunctionNitric oxide synthaseEthanol-fed miceSinusoidal endothelial dysfunctionPotential therapeutic approachPotential therapeutic strategyNitric oxide productionNew therapeutic strategiesSinusoidal endothelial cellsAcetylation of Hsp90
2020
Single-Cell Transcriptomics Reveals Zone-Specific Alterations of Liver Sinusoidal Endothelial Cells in Cirrhosis
Su T, Yang Y, Lai S, Jeong J, Jung Y, McConnell M, Utsumi T, Iwakiri Y. Single-Cell Transcriptomics Reveals Zone-Specific Alterations of Liver Sinusoidal Endothelial Cells in Cirrhosis. Cellular And Molecular Gastroenterology And Hepatology 2020, 11: 1139-1161. PMID: 33340713, PMCID: PMC7903131, DOI: 10.1016/j.jcmgh.2020.12.007.Peer-Reviewed Original ResearchConceptsLiver sinusoidal endothelial cellsCirrhotic miceSinusoidal endothelial cellsLiver cirrhosisEndothelial cellsIntrahepatic vascular resistanceCarbon tetrachloride inhalationNovel therapeutic strategiesNitric oxide productionCirrhotic mouse liverEC populationsVascular resistanceClinical complicationsLiver fibrosisTherapeutic strategiesCirrhosisOxide productionEndocytic receptorMiceAbstractTextZone 3Extracellular matrix genesVascular ECsLymphatic ECsMouse liverEnhanced Meningeal Lymphatic Drainage Ameliorates Neuroinflammation and Hepatic Encephalopathy in Cirrhotic Rats
Hsu SJ, Zhang C, Jeong J, Lee SI, McConnell M, Utsumi T, Iwakiri Y. Enhanced Meningeal Lymphatic Drainage Ameliorates Neuroinflammation and Hepatic Encephalopathy in Cirrhotic Rats. Gastroenterology 2020, 160: 1315-1329.e13. PMID: 33227282, PMCID: PMC7956141, DOI: 10.1053/j.gastro.2020.11.036.Peer-Reviewed Original ResearchConceptsMeningeal lymphatic drainageLymphatic drainageMicroglia activationMotor functionBile duct ligation modelTumor necrosis factor αSerious neurologic complicationsMeningeal lymphatic systemNecrosis factor αDuct ligation modelNew therapeutic strategiesBrain inflammationNeurologic complicationsHepatic encephalopathyLiver cirrhosisLymph nodesRotarod testMotor dysfunctionCirrhotic ratsInterleukin-1βLigation modelInterferon γProinflammatory genesCisterna magnaTherapeutic strategiesLymphatic Dysfunction as a Novel Therapeutic Target in Nonalcoholic Steatohepatitis
Jeong J, Iwakiri Y. Lymphatic Dysfunction as a Novel Therapeutic Target in Nonalcoholic Steatohepatitis. Cellular And Molecular Gastroenterology And Hepatology 2020, 11: 663-664. PMID: 33220266, PMCID: PMC7846486, DOI: 10.1016/j.jcmgh.2020.10.013.Commentaries, Editorials and Letters
2019
Lin28a expression protects against streptozotocin‐induced β‐cell destruction and prevents diabetes in mice
Sung Y, Jeong J, Kang R, Choi M, Park S, Kwon W, Lee J, Jang S, Park S, Kim S, Yi J, Choi S, Lee M, Liu K, Dong Z, Ryoo Z, Kim M. Lin28a expression protects against streptozotocin‐induced β‐cell destruction and prevents diabetes in mice. Cell Biochemistry And Function 2019, 37: 139-147. PMID: 30883865, DOI: 10.1002/cbf.3376.Peer-Reviewed Original ResearchConceptsGenome-wide association studiesCell growthΒ-cell destructionImportant regulatorLet-7 biogenesisLet-7 regulationPancreatic β-cell functionRole of Lin28Family of miRNAsRecent genome-wide association studiesΒ-cell functionPancreatic β-cell deathCell cycle progressionCell cycle regulatorsLin28/letΒ-cell deathOverexpression of Lin28aMiRNA biogenesisSkeletal myogenesisPrecise modulationEmbryonic developmentLin28a/Pancreatic β-cellsCycle regulatorsCycle progression
2018
Placental growth factor (PlGF) is linked to inflammation and metabolic disorders in mice with diet-induced obesity
Kang M, Jeong J, Lee J, Park S, Sung Y, Choi M, Kwon W, Jang S, Choi K, Choo Y, Yoon D, Kim M, Ryoo Z. Placental growth factor (PlGF) is linked to inflammation and metabolic disorders in mice with diet-induced obesity. Endocrine Journal 2018, 65: ej17-0363. PMID: 29434073, DOI: 10.1507/endocrj.ej17-0363.Peer-Reviewed Original ResearchConceptsHigh-fat dietDiet-induced obesityCD3/CD28 stimulationEpididymal white adipose tissueObesity-induced inflammationPlacental growth factorVascular endothelial growth factorT cellsMetabolic disordersTg miceT cell differentiationAdipose tissueHFD miceGrowth factorCD28 stimulationInflammatory T cell differentiationPro-inflammatory cytokines IL-6Mice fed high-fat dietType 17 helper T cellsFed high-fat dietWT-HFD miceSystemic metabolic disordersIsolated T lymphocytesHelper T cellsCytokines IL-6
2016
The Positive Effect of LuoHanGuo as Sugar Substitute on Blood Glucose and Metabolism in Streptozotocin-Induced Diabetic Mice
Lee Y, Jeong J, Kim M, Nam J. The Positive Effect of LuoHanGuo as Sugar Substitute on Blood Glucose and Metabolism in Streptozotocin-Induced Diabetic Mice. Applied Microscopy 2016, 46: 140-149. DOI: 10.9729/am.2016.46.3.140.Peer-Reviewed Original ResearchSerum amyloid A1 levels and amyloid deposition following a high-fat diet challenge in transgenic mice overexpressing hepatic serum amyloid A1
Jang W, Jeong J, Kim S, Kang M, Sung Y, Choi M, Park S, Kim M, Kim S, Ryoo Z. Serum amyloid A1 levels and amyloid deposition following a high-fat diet challenge in transgenic mice overexpressing hepatic serum amyloid A1. Applied Physiology Nutrition And Metabolism 2016, 41: 640-648. PMID: 27218680, DOI: 10.1139/apnm-2015-0369.Peer-Reviewed Original ResearchConceptsHigh-fat dietSAA1 levelsAmyloid depositionTg miceMetabolic disturbancesTransgenic miceHepatic serum amyloid A1Elevated levelsImpaired glucose toleranceLow-grade inflammationLocal cytokine expressionChronic inflammatory conditionsHigh-fat diet challengeSerum amyloid A1Acute phase response proteinsMajor precursor proteinCrohn's diseaseGlucose toleranceCytokine expressionInflammatory conditionsMetabolic parametersSerum amyloidMetabolic disordersAmyloid depositsDiet challenge
2013
Overexpression of Jazf1 reduces body weight gain and regulates lipid metabolism in high fat diet
Jang W, Bae K, Kim S, Yu D, Kim H, Ji Y, Park S, Park S, Kang M, Jeong J, Park S, Lee S, Lee I, Kim M, Yoon D, Ryoo Z. Overexpression of Jazf1 reduces body weight gain and regulates lipid metabolism in high fat diet. Biochemical And Biophysical Research Communications 2013, 444: 296-301. PMID: 24380856, DOI: 10.1016/j.bbrc.2013.12.094.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsBlood GlucoseCarrier ProteinsCo-Repressor ProteinsDiet, High-FatDNA-Binding ProteinsGlucose Tolerance TestHomeostasisInsulinLipid MetabolismMiceMice, TransgenicNuclear ProteinsPhosphoenolpyruvate Carboxykinase (GTP)Real-Time Polymerase Chain ReactionReverse Transcriptase Polymerase Chain ReactionWeight GainConceptsPutative zinc finger motifZinc finger motifsNuclear orphan receptor TR4Regulation of metabolismOverexpression of JAZF1Finger motifTranscription factorsNuclear proteinsKey enzymeExpression of JAZF1Lipid homeostasisFat dietNew therapeutic targetsWeight gainLipid accumulationManagement of obesityHigh-fat dietCritical roleJAZF1Lipid metabolismTherapeutic targetBody weight gainMetabolismRegulationDiabetes mellitus