2024
Identifying topologically associating domains using differential kernels
Maisuradze L, King M, Surovtsev I, Mochrie S, Shattuck M, O’Hern C. Identifying topologically associating domains using differential kernels. PLOS Computational Biology 2024, 20: e1012221. PMID: 39008525, PMCID: PMC11249266, DOI: 10.1371/journal.pcbi.1012221.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsAnimalsChromatinComputational BiologyHumansImage Processing, Computer-AssistedConceptsTopologically associating domainsHi-C mapsFalse discovery rateChromatin conformation capture techniquesEnhancer-promoter interactionsLow false discovery rateSelf-interacting regionsStructure of chromatinRegulate gene expressionAverage contact probabilitiesHi-CLocus IDNA transcriptionGene expressionChromatinDiscovery rateContact probabilityBiological phenomenaState-of-the-artKernel-based techniqueComputer visionReplicationCorrelated changesDisease statesCapture techniques
2015
Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis
Paintdakhi A, Parry B, Campos M, Irnov I, Elf J, Surovtsev I, Jacobs-Wagner C. Oufti: an integrated software package for high‐accuracy, high‐throughput quantitative microscopy analysis. Molecular Microbiology 2015, 99: 767-777. PMID: 26538279, PMCID: PMC4752901, DOI: 10.1111/mmi.13264.Peer-Reviewed Original ResearchMeSH KeywordsAlgorithmsBacteriaComputational BiologyData AccuracyHigh-Throughput Screening AssaysImage Processing, Computer-AssistedMicroscopy, FluorescenceSoftwareConceptsSingle-cell studiesOpen-source software packageGraphical user interfaceComplex subcellular organizationSoftware packageImage analysisUser interfaceMassive datasetsCellular functionsSubcellular organizationBacterial researchHigh-throughput analysisTouching cellsMicrobial cellsSubpixel precisionInteractive modulesComputational skillsComputational solutionPost-processing analysisPhenotypic variabilityFluorescence signalQuantitative microscopy analysisCell morphologyAutomated MeasurementConfluent samples
2014
Evidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation
Lim HC, Surovtsev IV, Beltran BG, Huang F, Bewersdorf J, Jacobs-Wagner C. Evidence for a DNA-relay mechanism in ParABS-mediated chromosome segregation. ELife 2014, 3: e02758. PMID: 24859756, PMCID: PMC4067530, DOI: 10.7554/elife.02758.Peer-Reviewed Original ResearchMeSH KeywordsBacterial ProteinsCaulobacter crescentusChromosome SegregationChromosomes, BacterialCulture MediaDNA-Binding ProteinsDNA, BacterialGenetic LociImage Processing, Computer-AssistedOptical ImagingConceptsChromosome segregationPartition complexParA-like proteinBacterial chromosome segregationDNA-binding activityParABS systemCytoplasmic cargoCaulobacter crescentusDNA regionsBiochemical approachesIntracellular transportTranslocation forceCellular parametersChromosomesTransient tethersCrescentusParABSMajor roleSegregationComplexesMechanismProteinTranslocationConservationBacteria