Featured Publications
Dectin-1 stimulation promotes a distinct inflammatory signature in the setting of HIV-infection and aging
Kumar A, Wang J, Esterly A, Radcliffe C, Zhou H, Wyk B, Allore H, Tsang S, Barakat L, Mohanty S, Zhao H, Shaw A, Zapata H. Dectin-1 stimulation promotes a distinct inflammatory signature in the setting of HIV-infection and aging. Aging 2023, 15: 7866-7908. PMID: 37606991, PMCID: PMC10497004, DOI: 10.18632/aging.204927.Peer-Reviewed Original ResearchConceptsDectin-1 stimulationDendritic cellsHIV-positive older adultsOlder adultsDectin-1 functionDistinct immune signaturesDistinct inflammatory signatureMonocytes of HIVCohort of HIVIFN-α productionPro-inflammatory environmentIFN-γ responsesPro-inflammatory phenotypeInnate immune receptorsDistinct gene signaturesStimulation of monocytesInflammatory signatureImmune signaturesHIV infectionIL-12Macrophage signatureCytokine productionIL-6Cytokine increaseHIV
2024
The quest to define senescence
Esterly A, Zapata H. The quest to define senescence. Frontiers In Genetics 2024, 15: 1396535. PMID: 38660674, PMCID: PMC11039885, DOI: 10.3389/fgene.2024.1396535.Peer-Reviewed Original Research
2023
Acute cytomegalovirus proctitis and epididymitis acquired via sexual transmission in an immunocompetent patient: a case report
Oyeyemi D, Chan E, Montano M, Belzer A, Ogbuagu O, Zapata H, Tuan J. Acute cytomegalovirus proctitis and epididymitis acquired via sexual transmission in an immunocompetent patient: a case report. Journal Of Medical Case Reports 2023, 17: 489. PMID: 37946248, PMCID: PMC10636986, DOI: 10.1186/s13256-023-04216-1.Peer-Reviewed Original ResearchConceptsCytomegalovirus proctitisCase reportAcute primary cytomegalovirus infectionPrimary cytomegalovirus infectionHealthy Caucasian individualsImmunocompetent personsTesticular painCytomegalovirus infectionImmunocompetent patientsRare causeImmunocompetent hostsSexual transmissionDiagnostic workupRectal pathologyConclusionsThis reportProctitisEpididymitisCaucasian individualsReportPainPatientsBackgroundWeSerologyWorkupInfectionBuilding an Infectious Disease Diversity, Equity, and Antiracism (ID2EA) Curriculum: A Single Center's Experience and Reflections
Gleeson S, Zapata H, Bathgate M, Emu B, Frederick J, Friedland G, Golden M, Meyer J, Radin J, Sideleau R, Shaw A, Shenoi S, Trubin P, Virata M, Barakat L, Desruisseaux M. Building an Infectious Disease Diversity, Equity, and Antiracism (ID2EA) Curriculum: A Single Center's Experience and Reflections. Clinical Infectious Diseases 2023, 77: 703-710. PMID: 37078888, DOI: 10.1093/cid/ciad236.Peer-Reviewed Original ResearchConceptsEducational activitiesHealthcare inequitiesInfectious Diseases SectionSingle-center experienceCoronavirus disease 2019 (COVID-19) pandemicDisease 2019 pandemicMixed-methods assessmentLearning objectivesPilot curriculumEducational trainingCurriculumCenter experienceLong-term behavioral changesProgram outcomesDisease sectionDisease diversityPhysician perspectivesResponse rateHealth disparitiesYale SchoolInequitiesAntiracismBehavioral changesRacismEquity
2022
Long-term quantitative assessment of anti-SARS-CoV-2 spike protein immunogenicity (QUASI) after COVID-19 vaccination in older people living with HIV (PWH)
Tuan JJ, Zapata H, Barakat L, Andrews L, Behnegar A, Kim YW, Kayani J, Mutic S, Ryall L, Turcotte B, Critch-Gilfillan T, Zhao M, Salahuddin S, Gupta S, Sutton R, Friedland G, Emu B, Ogbuagu O. Long-term quantitative assessment of anti-SARS-CoV-2 spike protein immunogenicity (QUASI) after COVID-19 vaccination in older people living with HIV (PWH). BMC Infectious Diseases 2022, 22: 744. PMID: 36131232, PMCID: PMC9491266, DOI: 10.1186/s12879-022-07737-0.Peer-Reviewed Original ResearchConceptsT cell responsesAnti-spike IgGVisit 3SARS-CoV-2Older PWHVisit 2Immune responseSingle-center longitudinal observational studyVirus-specific T cell responsesVaccine-induced humoral immunityLong-term protective immunityT cell immune responsesOlder peopleMedian age 61RBD IgG levelsPrimary study outcomeCOVID-19 vaccinationImmune response evaluationLongitudinal observational studyCOVID-19 vaccineWilcoxon signed-rank testBNT162b2 boosterBNT162b2 vaccinationCD8 responsesDetectable CD4
2021
Qualitative assessment of anti‐SARS‐CoV‐2 spike protein immunogenicity (QUASI) after COVID‐19 vaccination in older people living with HIV
Tuan JJ, Zapata H, Critch‐Gilfillan T, Ryall L, Turcotte B, Mutic S, Andrews L, Roh ME, Friedland G, Barakat L, Ogbuagu O. Qualitative assessment of anti‐SARS‐CoV‐2 spike protein immunogenicity (QUASI) after COVID‐19 vaccination in older people living with HIV. HIV Medicine 2021, 23: 178-185. PMID: 34632695, PMCID: PMC8652674, DOI: 10.1111/hiv.13188.Peer-Reviewed Original ResearchConceptsCOVID-19 vaccineCOVID-19 vaccinationFirst doseSeroconversion ratesSecond doseAcute respiratory syndrome coronavirus 2 infectionSevere acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infectionYale New Haven Health SystemPrior COVID-19 infectionSyndrome coronavirus 2 infectionSafe COVID-19 vaccineIntegrase strand transfer inhibitorsAnti-spike IgGPositive IgG responseTwo-dose seriesCoronavirus 2 infectionVaccine-induced immunityDurability of protectionCOVID-19 infectionStrand transfer inhibitorsAntiretroviral regimenBNT162b2 vaccineNew seroconversionsPositive IgGQualitative antibody
2020
Seasonal Variability and Shared Molecular Signatures of Inactivated Influenza Vaccination in Young and Older Adults
Avey S, Mohanty S, Chawla DG, Meng H, Bandaranayake T, Ueda I, Zapata HJ, Park K, Blevins TP, Tsang S, Belshe RB, Kaech SM, Shaw AC, Kleinstein SH. Seasonal Variability and Shared Molecular Signatures of Inactivated Influenza Vaccination in Young and Older Adults. The Journal Of Immunology 2020, 204: 1661-1673. PMID: 32060136, PMCID: PMC7755271, DOI: 10.4049/jimmunol.1900922.Peer-Reviewed Original ResearchMeSH KeywordsAdultAge FactorsAgedAgingAntibodies, ViralCohort StudiesFemaleGene Expression ProfilingHemagglutination Inhibition TestsHumansImmunogenicity, VaccineInfluenza A virusInfluenza VaccinesInfluenza, HumanMaleNK Cell Lectin-Like Receptor Subfamily BOligonucleotide Array Sequence AnalysisSeasonsTranscriptomeVaccinationVaccines, InactivatedYoung AdultConceptsVaccine-induced Ab responsesOlder adultsInfluenza vaccinationDays postvaccinationInfluenza vaccineAb responsesMore effective influenza vaccinesImportant public health toolInactivated influenza vaccinationSeasonal influenza vaccineVaccine-induced immunityEffective influenza vaccinesMolecular signaturesEffects of immunosenescencePublic health toolImmune signaturesVaccination seasonVaccine responsesVaccine compositionSubset of individualsAge groupsHealth toolsSingle age groupAdultsPostvaccination
2018
Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes
Zapata HJ, Van Ness PH, Avey S, Siconolfi B, Allore HG, Tsang S, Wilson J, Barakat L, Mohanty S, Shaw AC. Impact of Aging and HIV Infection on the Function of the C-Type Lectin Receptor MINCLE in Monocytes. The Journals Of Gerontology Series A 2018, 74: 794-801. PMID: 30239628, PMCID: PMC6521921, DOI: 10.1093/gerona/gly209.Peer-Reviewed Original ResearchConceptsPro-inflammatory environmentHIV infectionInnate immune pattern recognition receptorsPeripheral blood mononuclear cellsImmune pattern recognition receptorsC-type lectin receptorsCohort of HIVBlood mononuclear cellsMulticolor flow cytometryC-type lectin receptor MinclePattern recognition receptorsIL-10IL-12Cytokine productionIL-6Mononuclear cellsImmune responseHost responseCord factorFlow cytometryM. tuberculosisLectin receptorsMycobacterium tuberculosisOlder adultsImpact of aging
2017
Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses
Avey S, Cheung F, Fermin D, Frelinger J, Gaujoux R, Gottardo R, Khatri P, Kleinstein S, Kotliarov Y, Meng H, Sauteraud R, Shen-Orr S, Tsang J, Vallania F, Anguiano E, Baisch J, Baldwin N, Belshe R, Blevins T, Chaussabel D, Davis M, Fikrig E, Grill D, Hafler D, Henrich E, Joshi S, Kaech S, Kennedy R, Mohanty S, Montgomery R, Oberg A, Obermoser G, Ovsyannikova I, Palucka A, Pascual V, Poland G, Pulendran B, Reinherz E, Shaw A, Siconolfi B, Stuart K, Tsang S, Ueda I, Wilson J, Zapata H. Multicohort analysis reveals baseline transcriptional predictors of influenza vaccination responses. Science Immunology 2017, 2 PMID: 28842433, PMCID: PMC5800877, DOI: 10.1126/sciimmunol.aal4656.Peer-Reviewed Original ResearchAntibody responseInfluenza vaccination responsesVaccination responseHuman Immunology Project ConsortiumInfluenza vaccinationMulticohort analysisOlder individualsLower vaccine responsesSuccessful vaccination responseAnnual influenza vaccinationYoung individualsSubstantial antibody responseInflammatory gene signatureLarge independent studiesIndividuals 6 monthsGood antibody responsePublic health successImmune profiling studiesVaccination cohortVaccine responsesCell subsetsSmall cohortWorse responseVaccinationHealth successMultiple network-constrained regressions expand insights into influenza vaccination responses
Avey S, Mohanty S, Wilson J, Zapata H, Joshi SR, Siconolfi B, Tsang S, Shaw AC, Kleinstein SH. Multiple network-constrained regressions expand insights into influenza vaccination responses. Bioinformatics 2017, 33: i208-i216. PMID: 28881994, PMCID: PMC5870750, DOI: 10.1093/bioinformatics/btx260.Peer-Reviewed Original Research
2015
The Microbiota and Microbiome in Aging: Potential Implications in Health and Age‐Related Diseases
Zapata HJ, Quagliarello VJ. The Microbiota and Microbiome in Aging: Potential Implications in Health and Age‐Related Diseases. Journal Of The American Geriatrics Society 2015, 63: 776-781. PMID: 25851728, PMCID: PMC4406803, DOI: 10.1111/jgs.13310.Peer-Reviewed Original ResearchConceptsOlder adultsClostridium difficile colitisDevelopment of comorbiditiesCommensal bacterial communityDifficile colitisVulvovaginal atrophyProinflammatory stateAtherosclerotic diseaseClinical changesColorectal carcinomaPhysical frailtyClinical problemLocation of residenceGut microbiotaDeoxyribonucleic acid sequencingHealthy adultsAccompany agingBasal levelsAdultsMicrobiotaDiseaseMicrobiomeColitisComorbiditiesPotential implicationsIncreased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers
Walker WE, Kurscheid S, Joshi S, Lopez CA, Goh G, Choi M, Barakat L, Francis J, Fisher A, Kozal M, Zapata H, Shaw A, Lifton R, Sutton RE, Fikrig E. Increased Levels of Macrophage Inflammatory Proteins Result in Resistance to R5-Tropic HIV-1 in a Subset of Elite Controllers. Journal Of Virology 2015, 89: 5502-5514. PMID: 25740989, PMCID: PMC4442529, DOI: 10.1128/jvi.00118-15.Peer-Reviewed Original ResearchMeSH KeywordsAdultAgedCase-Control StudiesCD4-Positive T-LymphocytesChemokine CCL3Chemokine CCL4Chemokine CCL5Chemokines, CCCohort StudiesFemaleGene DosageHIV InfectionsHIV Long-Term SurvivorsHIV-1Host-Pathogen InteractionsHumansMacrophage Inflammatory ProteinsMaleMiddle AgedReceptors, CCR5Receptors, CXCR4RNA, MessengerUp-RegulationConceptsElite controllersHIV-seropositive individualsAntiretroviral therapyT cellsMIP-1βMIP-1αHIV infectionSeropositive individualsAbsence of ARTR5-tropic HIV-1Macrophage inflammatory protein-1αR5-tropic HIVInflammatory protein-1αT cell resistancePandemic health problemRANTES chemokinesHIV replicationRare patientsHealthy controlsTherapeutic effectHIV entryHIV-1X4-tropicHealth problemsProtein-1α
2014
Aging of the human innate immune system in HIV infection
Zapata HJ, Shaw AC. Aging of the human innate immune system in HIV infection. Current Opinion In Immunology 2014, 29: 127-136. PMID: 24997358, PMCID: PMC4145838, DOI: 10.1016/j.coi.2014.06.007.Peer-Reviewed Original ResearchConceptsHIV infectionHIV-negative adultsChronic inflammatory statePro-inflammatory environmentInnate immune recognitionInnate immune responseInnate immune systemHuman innate immune systemMicrobial translocationImmune agingInflammatory stateImmune responseEndogenous ligandImmune recognitionImmune systemInfectionMultiple factorsEarly stagesHIVControl of Simultaneous Outbreaks of Carbapenemase-Producing Enterobacteriaceae and Extensively Drug-Resistant Acinetobacter baumannii Infection in an Intensive Care Unit Using Interventions Promoted in the Centers for Disease Control and Prevention 2012 Carbapenemase-Resistant Enterobacteriaceae Toolkit
Enfield KB, Huq NN, Gosseling MF, Low DJ, Hazen KC, Toney DM, Slitt G, Zapata HJ, Cox HL, Lewis JD, Kundzins JR, Mathers AJ, Sifri CD. Control of Simultaneous Outbreaks of Carbapenemase-Producing Enterobacteriaceae and Extensively Drug-Resistant Acinetobacter baumannii Infection in an Intensive Care Unit Using Interventions Promoted in the Centers for Disease Control and Prevention 2012 Carbapenemase-Resistant Enterobacteriaceae Toolkit. Infection Control And Hospital Epidemiology 2014, 35: 810-817. PMID: 24915208, DOI: 10.1086/676857.Peer-Reviewed Original ResearchMeSH KeywordsAcademic Medical CentersAcinetobacter baumanniiAcinetobacter InfectionsCarbapenemsCenters for Disease Control and Prevention, U.S.Cross InfectionDrug Resistance, Multiple, BacterialEnterobacteriaceaeEnterobacteriaceae InfectionsHumansInfection ControlIntensive Care UnitsOutcome Assessment, Health CareUnited StatesVirginiaConceptsInfection control measuresIntensive care unitCarbapenemase-producing EnterobacteriaceaeXDR-ABIncidence rateDisease controlCare unitDrug-resistant Acinetobacter baumannii infectionsSurgical trauma intensive care unitTrauma intensive care unitDrug-resistant Acinetobacter baumanniiPresence of blaKPCXDR-AB infectionsXDR-AB isolatesAcinetobacter baumannii infectionsResistance plasmidsControl measuresBaumannii infectionsPolymerase chain reactionPulsed-field gel electrophoresisAcinetobacter baumanniiIntervention studiesConcurrent outbreaksMore casesOngoing transmission
2013
Initial diagnosis of HIV/AIDS in a 56-year-old man with non-healing forearm lesion
Zapata HJ, Villanueva M, Shenoi S. Initial diagnosis of HIV/AIDS in a 56-year-old man with non-healing forearm lesion. BMJ Case Reports 2013, 2013: bcr2013201184. PMID: 24248316, PMCID: PMC3841407, DOI: 10.1136/bcr-2013-201184.Peer-Reviewed Case Reports and Technical NotesConceptsForearm lesionPatient underwent incisionHIV viral loadMonths of treatmentSignificant medical problemHIV/AIDSEmpiric antibioticsAntiretroviral therapyCD4 countUnderwent incisionInitial diagnosisViral loadStarry stainImmunohistochemistry testingCopies/Bacillary angiomatosisHispanic menMedical problemsLesionsBartonella quintanaCells/PatientsMenHIVAngiomatosis
2011
Conserved Residues in the Extracellular Loops of Short-Wavelength Cone Visual Pigments
Chen MH, Sandberg DJ, Babu KR, Bubis J, Surya A, Ramos LS, Zapata HJ, Galan JF, Sandberg MN, Birge RR, Knox BE. Conserved Residues in the Extracellular Loops of Short-Wavelength Cone Visual Pigments. Biochemistry 2011, 50: 6763-6773. PMID: 21688771, PMCID: PMC3518856, DOI: 10.1021/bi101557m.Peer-Reviewed Original Research
2009
Listerial Rhomboencephalitis: Not Just for the Weakened Immune System
Zapata H, Bose A, Javaid W, Blair D. Listerial Rhomboencephalitis: Not Just for the Weakened Immune System. Clinical Microbiology Newsletter 2009, 31: 108-110. DOI: 10.1016/j.clinmicnews.2009.06.004.Peer-Reviewed Original Research
2006
Varicella-Zoster Virus Infection of Human Fibroblast Cells Activates the c-Jun N-Terminal Kinase Pathway
Zapata HJ, Nakatsugawa M, Moffat JF. Varicella-Zoster Virus Infection of Human Fibroblast Cells Activates the c-Jun N-Terminal Kinase Pathway. Journal Of Virology 2006, 81: 977-990. PMID: 17079291, PMCID: PMC1797429, DOI: 10.1128/jvi.01470-06.Peer-Reviewed Original ResearchConceptsPhospho-c-JunC-JunPhospho-JNKKinase pathwayC-Jun N-terminal kinaseC-Jun N-terminal kinase (JNK) pathwayTranscription factor ATF-2N-terminal kinase pathwayProtein kinase pathwayImmunofluorescent confocal microscopyN-terminal kinaseBiochemical fractionationKinase assaysUseful drug targetC-Jun levelsATF-2Phosphorylated formVirus genesJNK pathwayDrug targetsHuman fibroblast cellsPhosphorylated JNKJNKMain activatorHuman foreskin fibroblasts