Nuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*
Yao G, Sun B, Insogna K, Weir E. Nuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*. Endocrinology 2000, 141: 2914-2922. PMID: 10919279, DOI: 10.1210/endo.141.8.7592.Peer-Reviewed Original ResearchConceptsCSF-1 expressionGene expressionElectrophoretic mobility shift assaysCSF-1 promoterCSF-1 gene expressionMobility shift assaysInducible complexTranscriptional mechanismsShift assaysNuclear factor-κB p50Northern analysisNF-kappaB p50NF-kappaB siteCSF-1Messenger RNAC-RelBcl-3Rel BRNA expressionTNF treatmentHematopoietic growth factorsMessenger RNA expressionIkappaB-alphaOsteoblastsNF-kappaBNuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*
Yao G, Sun B, Insogna K, Weir E. Nuclear Factor-κB p50 Is Required for Tumor Necrosis Factor-α-Induced Colony-Stimulating Factor-1 Gene Expression in Osteoblasts*. Endocrinology 2000, 141: 2914-2922. DOI: 10.1210/en.141.8.2914.Peer-Reviewed Original ResearchCSF-1 expressionKnock-out miceCSF-1NF-kBColony-stimulating factor (CSF)-1Gene expressionColony-stimulating factor-1 gene expressionCSF-1 gene expressionElectrophoretic mobility shift assayTumor necrosis factor (TNF)-aBone-resorbing agentsHematopoietic growth factorsWild-type miceMobility shift assayCSF-1 promoterBone remodeling in vivoNF-kB bindingNF-kB sitesMessenger RNA expressionShift assaysRemodeling in vivoTranscriptional mechanismsNorthern analysisBcl-3C-Rel