2015
A structural model for facultative anion channels in an oligomeric membrane protein: the yeast TRK (K+) system
Pardo JP, González-Andrade M, Allen K, Kuroda T, Slayman CL, Rivetta A. A structural model for facultative anion channels in an oligomeric membrane protein: the yeast TRK (K+) system. Pflügers Archiv - European Journal Of Physiology 2015, 467: 2447-2460. PMID: 26100673, DOI: 10.1007/s00424-015-1712-6.Peer-Reviewed Original ResearchConceptsTransmembrane helicesAnion channelTrk proteinNon-animal cellsOligomeric membrane proteinsAmphipathic transmembrane helicesLigand-gated anion channelsClass of proteinsTrk transportersRCK domainsBacterial membersRegulatory domainMembrane proteinsFungal proteinsTrk systemHydrophobic gatingPrimary sequenceMembrane voltageBiological membranesProteinCytoplasmic collarFunctional processesChloride effluxHelixPathway
2013
Coordination of K+ Transporters in Neurospora: TRK1 Is Scarce and Constitutive, while HAK1 Is Abundant and Highly Regulated
Rivetta A, Allen KE, Slayman CW, Slayman CL. Coordination of K+ Transporters in Neurospora: TRK1 Is Scarce and Constitutive, while HAK1 Is Abundant and Highly Regulated. MSphere 2013, 12: 684-696. PMID: 23475706, PMCID: PMC3647778, DOI: 10.1128/ec.00017-13.Peer-Reviewed Original ResearchConceptsTransmembrane helicesCarbon starvationModel organism Neurospora crassaPotassium transportersNeurospora crassaClass proteinsQuantitative Western blottingATP hydrolysisPotassium limitationTRK1Molecular machinesHAK1Trk1pWestern blottingPotassium channelsCoexpressionStarvationTransportersHelixTransporter affinityElectrophysiological characterizationExpressionMM/hCrassaNeurospora
2011
Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels
Rivetta A, Kuroda T, Slayman C. Anion currents in yeast K+ transporters (TRK) characterize a structural homologue of ligand-gated ion channels. Pflügers Archiv - European Journal Of Physiology 2011, 462: 315-330. PMID: 21556692, PMCID: PMC3151154, DOI: 10.1007/s00424-011-0959-9.Peer-Reviewed Original Research
2009
Conservation and dispersion of sequence and function in fungal TRK potassium transporters: focus on Candida albicans
Miranda M, Bashi E, Vylkova S, Edgerton M, Slayman C, Rivetta A. Conservation and dispersion of sequence and function in fungal TRK potassium transporters: focus on Candida albicans. FEMS Yeast Research 2009, 9: 278-292. PMID: 19175416, DOI: 10.1111/j.1567-1364.2008.00471.x.Peer-Reviewed Original ResearchMeSH KeywordsCandida albicansCation Transport ProteinsChloridesConserved SequenceFungal ProteinsModels, BiologicalModels, MolecularPhylogenyPolymorphism, Single NucleotidePotassiumProtein ConformationProtein Structure, TertiarySaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence Homology, Amino AcidConceptsPotassium transportersHuman pathogen Candida albicansTrk potassium transportersPathogen Candida albicansDetailed molecular investigationAnimal cellsSingle nucleotide polymorphismsDNA sequencesS. cerevisiaeComplete sequenceC. albicansTrk proteinSequence analysisCandida albicansInhibitor sensitivityFunctional comparisonNucleotide polymorphismsSelective drug actionMolecular investigationsAntimicrobial peptidesHomologuesPotential targetSecondary functionSalivary antimicrobial peptidesProtein
2004
The TRK1 Potassium Transporter Is the Critical Effector for Killing of Candida albicans by the Cationic Protein, Histatin 5*
Baev D, Rivetta A, Vylkova S, Sun JN, Zeng GF, Slayman CL, Edgerton M. The TRK1 Potassium Transporter Is the Critical Effector for Killing of Candida albicans by the Cationic Protein, Histatin 5*. Journal Of Biological Chemistry 2004, 279: 55060-55072. PMID: 15485849, DOI: 10.1074/jbc.m411031200.Peer-Reviewed Original ResearchMeSH Keywords4-Acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic AcidAdenosine TriphosphateAllelesAnionsAntifungal AgentsAntimicrobial Cationic PeptidesBlotting, WesternCandida albicansCation Transport ProteinsCationsCell MembraneCell SeparationChloride ChannelsChloridesCytoplasmDNA PrimersDNA, ComplementaryDose-Response Relationship, DrugElectrophysiologyEscherichia coliFlow CytometryGene DeletionGenetic Complementation TestHistatinsHistidineModels, ChemicalModels, GeneticOligonucleotidesOpen Reading FramesPatch-Clamp TechniquesPlasmidsPotassiumProtease InhibitorsProtein BindingProtein Structure, TertiaryReverse Transcriptase Polymerase Chain ReactionRNARubidiumSaccharomyces cerevisiae ProteinsSalivary Proteins and PeptidesTime FactorsConceptsHst 5Hst 5 toxicityCritical effectorWild-type cellsTrk1 potassium transporterC. albicansPotassium transportersDiploid organismsOverexpression strainSingle copyCytoplasmic sequestrationPlasma membraneHistatin 5Essential pathwayPathogenic fungiCandida albicansAnion channel inhibitorsGenesTrk1pProteinATP lossChloride conductanceSmall moleculesEffectorsPossible roleChloride Channel Function in the Yeast TRK-Potassium Transporters
Kuroda T, Bihler H, Bashi E, Slayman CL, Rivetta A. Chloride Channel Function in the Yeast TRK-Potassium Transporters. The Journal Of Membrane Biology 2004, 198: 177-192. PMID: 15216418, DOI: 10.1007/s00232-004-0671-1.Peer-Reviewed Original Research