2015
Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*
Smith KD, Gordon PB, Rivetta A, Allen KE, Berbasova T, Slayman C, Strobel SA. Yeast Fex1p Is a Constitutively Expressed Fluoride Channel with Functional Asymmetry of Its Two Homologous Domains*. Journal Of Biological Chemistry 2015, 290: 19874-19887. PMID: 26055717, PMCID: PMC4528147, DOI: 10.1074/jbc.m115.651976.Peer-Reviewed Original ResearchMeSH KeywordsAmino Acid SequenceCell MembraneConserved SequenceDrug Resistance, FungalEscherichia coliFluoridesGene ExpressionGene Expression Regulation, FungalGenome, FungalIon TransportMembrane ProteinsMolecular Sequence DataMutationPatch-Clamp TechniquesPhosphorylationPhylogenyProtein FoldingProtein MultimerizationProtein Structure, SecondaryProtein Structure, TertiaryRecombinant ProteinsSaccharomyces cerevisiaeSaccharomyces cerevisiae ProteinsSequence AlignmentStatic ElectricityConceptsC-terminal domainSaccharomyces cerevisiae functionsYeast plasma membraneN-terminal domainEffects of mutationsDuplicate proteinsYeast genomeCerevisiae functionsSequence conservationHelix domainLinker helixUbiquitous environmental toxinHomologous domainsImportant residuesFluoride channelsPlasma membraneParticular residuesBiological speciesSimilar mutationsIon channelsContinuous expressionProteinAntiparallel dimerMutationsResidues
1998
Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae
Bertl A, Bihler H, Kettner C, Slayman CL. Electrophysiology in the eukaryotic model cell Saccharomyces cerevisiae. Pflügers Archiv - European Journal Of Physiology 1998, 436: 999-1013. PMID: 9799419, DOI: 10.1007/s004240050735.Peer-Reviewed Original ResearchConceptsYeast plasma membranePlasma membraneMembrane proteinsYeast membranesFunctional analysisHeterologous membrane proteinsFacile genetic manipulationDetailed functional analysisPreliminary functional analysisYeast vacuolar membraneGigaseal formationHeterologous genesYeast vacuoleCytoplasmic proteinsVacuolar membraneFungal proteinsGenetic manipulationSpecific proteinsPolyploid strainsSaccharomycesNative proteinYeastProtoplastsIon channelsProtein