2023
Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery
Sundaram R, Chatterjee A, Bera M, Grushin K, Panda A, Li F, Coleman J, Lee S, Ramakrishnan S, Ernst A, Gupta K, Rothman J, Krishnakumar S. Roles for diacylglycerol in synaptic vesicle priming and release revealed by complete reconstitution of core protein machinery. Proceedings Of The National Academy Of Sciences Of The United States Of America 2023, 120: e2309516120. PMID: 37590407, PMCID: PMC10450444, DOI: 10.1073/pnas.2309516120.Peer-Reviewed Original ResearchConceptsCore protein machineryRelease-ready vesiclesSynaptic vesicle primingVesicle primingProtein machinerySingle-molecule imagingSNAREpin assemblyFunctional intermediatesFunctional reconstitutionMunc13DiacylglycerolCoordinated actionMunc18VesiclesMachineryComplete reconstitutionNew roleSelective effectDetailed characterizationChaperonesRate of caReconstitutionVAMP2ComplexinMutations
2018
Functional analysis of mammalian phospholipase D enzymes
Panda A, Thakur R, Krishnan H, Naik A, Shinde D, Raghu P. Functional analysis of mammalian phospholipase D enzymes. Bioscience Reports 2018, 38: bsr20181690. PMID: 30369483, PMCID: PMC6435507, DOI: 10.1042/bsr20181690.Peer-Reviewed Original ResearchConceptsHuman PLD2Human PLD1Phospholipase DMammalian genomesPLD genesPhosphatidic acidPA speciesSame biochemical activityMammalian phospholipase DSubplasma membrane regionPhotoreceptor plasma membraneSpecific phospholipase DInvertebrate genomesDistinct subcellular membranesAcyl chain compositionLoss of functionPhylogenetic analysisVesicular transportUnique speciesPlasma membraneSubcellular functionsDistinct isoformsFunctional analysisGenomeBiochemical activity