2022
Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry.
Panda A, Thakur R, Kumari A, Raghu P. Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry. Journal Of Visualized Experiments 2022 PMID: 35311809, DOI: 10.3791/63516.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDrosophilaDrosophila melanogasterDrosophila ProteinsMass SpectrometryPhosphatidylinositolsPhotoreceptor Cells, InvertebrateConceptsTransient receptor potentialDrosophila photoreceptorsSensory transductionMembrane lipid phosphatidylinositolLipid mass spectrometryLipid phosphatidylinositolDrosophila mutantsLipid signalingSignaling lipidsClasses of lipidsCell biologyPhospholipase CβSpecific lipidsMolecular geneticsLipid speciesPhotoreceptor physiologySuch lipidsMass spectrometryMutantsPhotoreceptorsLipid analysisTransductionModel systemBiologyReceptor potential
2018
Evidence of sinks and sources in the phospholipase C‐activated PIP2 cycle
Suratekar R, Panda A, Raghu P, Krishna S. Evidence of sinks and sources in the phospholipase C‐activated PIP2 cycle. FEBS Letters 2018, 592: 962-972. PMID: 29427502, DOI: 10.1002/1873-3468.12998.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsDrosophila melanogasterDrosophila ProteinsModels, BiologicalPhosphatidylinositol 4,5-DiphosphateSignal TransductionType C Phospholipases
2016
Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling
Thakur R, Panda A, Coessens E, Raj N, Yadav S, Balakrishnan S, Zhang Q, Georgiev P, Basak B, Pasricha R, Wakelam M, Ktistakis N, Raghu P. Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. ELife 2016, 5: e18515. PMID: 27848911, PMCID: PMC5125754, DOI: 10.7554/elife.18515.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation Factor 1AnimalsCell MembraneCytoplasmic VesiclesDrosophila melanogasterDrosophila ProteinsEndocytosisGene ExpressionGenetic Complementation TestGuanosine TriphosphateLightPhosphatidic AcidsPhospholipase DPhotic StimulationPhotoreceptor Cells, InvertebrateRab GTP-Binding ProteinsRab7 GTP-Binding ProteinsRhodopsinVision, OcularConceptsPlasma membranePLD activityRetromer-dependent recyclingWild-type photoreceptorsMembrane endocytosisDependent recyclingPhospholipase D activityPhosphatidic acidRhabdomere sizeRhodopsin proteinEndocytosisCell bodiesPhotoreceptorsReduced levelsActivity couplesD activityComplex functionsMembraneTurnoverRab7ProteinVesiclesPhenotypeConsequent changesActivity
2015
RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction
Yadav S, Garner K, Georgiev P, Li M, Gomez-Espinosa E, Panda A, Mathre S, Okkenhaug H, Cockcroft S, Raghu P. RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction. Journal Of Cell Science 2015, 128: 3330-3344. PMID: 26203165, PMCID: PMC4582195, DOI: 10.1242/jcs.173476.Peer-Reviewed Original ResearchConceptsPhospholipase CPlasma membraneEndoplasmic reticulumMulti-domain proteinsPlasma membrane lipidsExchange of lipidsRdgB functionDrosophila phototransductionDrosophila photoreceptorsProtein domainsRdgB mutantsVivo functionMembrane lipidsPtdInsLipid intermediatesLipid homeostasisMembrane receptorsPLC activationPtdOH levelsTransfer proteinPhosphatidic acidPLC activityPhototransductionNormal phototransductionPtdOH