2022
Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry.
Panda A, Thakur R, Kumari A, Raghu P. Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry. Journal Of Visualized Experiments 2022 PMID: 35311809, DOI: 10.3791/63516.Peer-Reviewed Original ResearchConceptsTransient receptor potentialDrosophila photoreceptorsSensory transductionMembrane lipid phosphatidylinositolLipid mass spectrometryLipid phosphatidylinositolDrosophila mutantsLipid signalingSignaling lipidsClasses of lipidsCell biologyPhospholipase CβSpecific lipidsMolecular geneticsLipid speciesPhotoreceptor physiologySuch lipidsMass spectrometryMutantsPhotoreceptorsLipid analysisTransductionModel systemBiologyReceptor potential
2018
Functional analysis of mammalian phospholipase D enzymes
Panda A, Thakur R, Krishnan H, Naik A, Shinde D, Raghu P. Functional analysis of mammalian phospholipase D enzymes. Bioscience Reports 2018, 38: bsr20181690. PMID: 30369483, PMCID: PMC6435507, DOI: 10.1042/bsr20181690.Peer-Reviewed Original ResearchConceptsHuman PLD2Human PLD1Phospholipase DMammalian genomesPLD genesPhosphatidic acidPA speciesSame biochemical activityMammalian phospholipase DSubplasma membrane regionPhotoreceptor plasma membraneSpecific phospholipase DInvertebrate genomesDistinct subcellular membranesAcyl chain compositionLoss of functionPhylogenetic analysisVesicular transportUnique speciesPlasma membraneSubcellular functionsDistinct isoformsFunctional analysisGenomeBiochemical activity