2016
Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling
Thakur R, Panda A, Coessens E, Raj N, Yadav S, Balakrishnan S, Zhang Q, Georgiev P, Basak B, Pasricha R, Wakelam M, Ktistakis N, Raghu P. Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. ELife 2016, 5: e18515. PMID: 27848911, PMCID: PMC5125754, DOI: 10.7554/elife.18515.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation Factor 1AnimalsCell MembraneCytoplasmic VesiclesDrosophila melanogasterDrosophila ProteinsEndocytosisGene ExpressionGenetic Complementation TestGuanosine TriphosphateLightPhosphatidic AcidsPhospholipase DPhotic StimulationPhotoreceptor Cells, InvertebrateRab GTP-Binding ProteinsRab7 GTP-Binding ProteinsRhodopsinVision, OcularConceptsPlasma membranePLD activityRetromer-dependent recyclingWild-type photoreceptorsMembrane endocytosisDependent recyclingPhospholipase D activityPhosphatidic acidRhabdomere sizeRhodopsin proteinEndocytosisCell bodiesPhotoreceptorsReduced levelsActivity couplesD activityComplex functionsMembraneTurnoverRab7ProteinVesiclesPhenotypeConsequent changesActivity
2015
Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins
Raghupathy R, Anilkumar A, Polley A, Singh P, Yadav M, Johnson C, Suryawanshi S, Saikam V, Sawant S, Panda A, Guo Z, Vishwakarma R, Rao M, Mayor S. Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins. Cell 2015, 161: 581-594. PMID: 25910209, PMCID: PMC4651428, DOI: 10.1016/j.cell.2015.03.048.Peer-Reviewed Original ResearchConceptsLive cell membranesCell membraneDynamic actin filamentsInner leafletLiquid-ordered phaseAtom molecular dynamics simulationsActin filamentsLipid domainsGeneral mechanismTransbilayer couplingOpposite leafletLipid clustersAcyl chain lipidsProteinPhosphatidylserineLipidsMembraneMolecular dynamics simulationsLeafletsNanoclusteringCorresponding lipidsDomainGPIInteractionDynamics simulations