2019
Regulation of Membrane Turnover by Phosphatidic Acid: Cellular Functions and Disease Implications
Thakur R, Naik A, Panda A, Raghu P. Regulation of Membrane Turnover by Phosphatidic Acid: Cellular Functions and Disease Implications. Frontiers In Cell And Developmental Biology 2019, 7: 83. PMID: 31231646, PMCID: PMC6559011, DOI: 10.3389/fcell.2019.00083.Peer-Reviewed Original ResearchEukaryotic cellsPhosphatidic acidHuman disease geneticsPA functionSingle cell typeNovel genetic modelModel organismsSimplest glycerophospholipidCellular functionsDisease geneticsSignal transductionLipid biosynthesisPhospholipid biosynthesisCell biologyPLD inhibitorsMembrane transportHuman diseasesCell typesDisease implicationsMembrane turnoverGenetic modelsBiosynthesisProteinEnzymeCells
2018
Functional analysis of mammalian phospholipase D enzymes
Panda A, Thakur R, Krishnan H, Naik A, Shinde D, Raghu P. Functional analysis of mammalian phospholipase D enzymes. Bioscience Reports 2018, 38: bsr20181690. PMID: 30369483, PMCID: PMC6435507, DOI: 10.1042/bsr20181690.Peer-Reviewed Original ResearchConceptsHuman PLD2Human PLD1Phospholipase DMammalian genomesPLD genesPhosphatidic acidPA speciesSame biochemical activityMammalian phospholipase DSubplasma membrane regionPhotoreceptor plasma membraneSpecific phospholipase DInvertebrate genomesDistinct subcellular membranesAcyl chain compositionLoss of functionPhylogenetic analysisVesicular transportUnique speciesPlasma membraneSubcellular functionsDistinct isoformsFunctional analysisGenomeBiochemical activity
2016
Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling
Thakur R, Panda A, Coessens E, Raj N, Yadav S, Balakrishnan S, Zhang Q, Georgiev P, Basak B, Pasricha R, Wakelam M, Ktistakis N, Raghu P. Phospholipase D activity couples plasma membrane endocytosis with retromer dependent recycling. ELife 2016, 5: e18515. PMID: 27848911, PMCID: PMC5125754, DOI: 10.7554/elife.18515.Peer-Reviewed Original ResearchMeSH KeywordsADP-Ribosylation Factor 1AnimalsCell MembraneCytoplasmic VesiclesDrosophila melanogasterDrosophila ProteinsEndocytosisGene ExpressionGenetic Complementation TestGuanosine TriphosphateLightPhosphatidic AcidsPhospholipase DPhotic StimulationPhotoreceptor Cells, InvertebrateRab GTP-Binding ProteinsRab7 GTP-Binding ProteinsRhodopsinVision, OcularConceptsPlasma membranePLD activityRetromer-dependent recyclingWild-type photoreceptorsMembrane endocytosisDependent recyclingPhospholipase D activityPhosphatidic acidRhabdomere sizeRhodopsin proteinEndocytosisCell bodiesPhotoreceptorsReduced levelsActivity couplesD activityComplex functionsMembraneTurnoverRab7ProteinVesiclesPhenotypeConsequent changesActivity
2015
RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction
Yadav S, Garner K, Georgiev P, Li M, Gomez-Espinosa E, Panda A, Mathre S, Okkenhaug H, Cockcroft S, Raghu P. RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction. Journal Of Cell Science 2015, 128: 3330-3344. PMID: 26203165, PMCID: PMC4582195, DOI: 10.1242/jcs.173476.Peer-Reviewed Original ResearchConceptsPhospholipase CPlasma membraneEndoplasmic reticulumMulti-domain proteinsPlasma membrane lipidsExchange of lipidsRdgB functionDrosophila phototransductionDrosophila photoreceptorsProtein domainsRdgB mutantsVivo functionMembrane lipidsPtdInsLipid intermediatesLipid homeostasisMembrane receptorsPLC activationPtdOH levelsTransfer proteinPhosphatidic acidPLC activityPhototransductionNormal phototransductionPtdOH