2023
Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer
Panda A, Giska F, Duncan A, Welch A, Brown C, McAllister R, Hariharan P, Goder J, Coleman J, Ramakrishnan S, Pincet F, Guan L, Krishnakumar S, Rothman J, Gupta K. Direct determination of oligomeric organization of integral membrane proteins and lipids from intact customizable bilayer. Nature Methods 2023, 20: 891-897. PMID: 37106230, PMCID: PMC10932606, DOI: 10.1038/s41592-023-01864-5.Peer-Reviewed Original ResearchConceptsIntegral membrane proteinsMembrane proteinsOligomeric organizationOligomeric stateNative mass spectrometry analysisFunctional oligomeric stateKey membrane componentMass spectrometry analysisNMS analysisTarget membraneLipid bindingMembrane componentsProteolipid vesiclesMembrane compositionLipid compositionSpectrometry analysisLipid membranesNeurotransmitter releaseProteinMembraneLipidsMembrane propertiesDirect determinationBilayersTransporters
2022
A tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membrane
Panda A, Giska F, Brown C, Coleman J, Rothman J, Gupta K. A tunable lipid bilayer native MS platform for direct determination of hierarchical organization of membrane proteins and lipids at the membrane. Biophysical Journal 2022, 121: 312a-313a. DOI: 10.1016/j.bpj.2021.11.1192.Peer-Reviewed Original Research
2015
Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins
Raghupathy R, Anilkumar A, Polley A, Singh P, Yadav M, Johnson C, Suryawanshi S, Saikam V, Sawant S, Panda A, Guo Z, Vishwakarma R, Rao M, Mayor S. Transbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins. Cell 2015, 161: 581-594. PMID: 25910209, PMCID: PMC4651428, DOI: 10.1016/j.cell.2015.03.048.Peer-Reviewed Original ResearchConceptsLive cell membranesCell membraneDynamic actin filamentsInner leafletLiquid-ordered phaseAtom molecular dynamics simulationsActin filamentsLipid domainsGeneral mechanismTransbilayer couplingOpposite leafletLipid clustersAcyl chain lipidsProteinPhosphatidylserineLipidsMembraneMolecular dynamics simulationsLeafletsNanoclusteringCorresponding lipidsDomainGPIInteractionDynamics simulations