2022
Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry.
Panda A, Thakur R, Kumari A, Raghu P. Analysis of Lipid Signaling in Drosophila Photoreceptors using Mass Spectrometry. Journal Of Visualized Experiments 2022 PMID: 35311809, DOI: 10.3791/63516.Peer-Reviewed Original ResearchConceptsTransient receptor potentialDrosophila photoreceptorsSensory transductionMembrane lipid phosphatidylinositolLipid mass spectrometryLipid phosphatidylinositolDrosophila mutantsLipid signalingSignaling lipidsClasses of lipidsCell biologyPhospholipase CβSpecific lipidsMolecular geneticsLipid speciesPhotoreceptor physiologySuch lipidsMass spectrometryMutantsPhotoreceptorsLipid analysisTransductionModel systemBiologyReceptor potential
2015
RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction
Yadav S, Garner K, Georgiev P, Li M, Gomez-Espinosa E, Panda A, Mathre S, Okkenhaug H, Cockcroft S, Raghu P. RDGBα, a PtdIns-PtdOH transfer protein, regulates G-protein-coupled PtdIns(4,5)P2 signalling during Drosophila phototransduction. Journal Of Cell Science 2015, 128: 3330-3344. PMID: 26203165, PMCID: PMC4582195, DOI: 10.1242/jcs.173476.Peer-Reviewed Original ResearchConceptsPhospholipase CPlasma membraneEndoplasmic reticulumMulti-domain proteinsPlasma membrane lipidsExchange of lipidsRdgB functionDrosophila phototransductionDrosophila photoreceptorsProtein domainsRdgB mutantsVivo functionMembrane lipidsPtdInsLipid intermediatesLipid homeostasisMembrane receptorsPLC activationPtdOH levelsTransfer proteinPhosphatidic acidPLC activityPhototransductionNormal phototransductionPtdOH