2020
Two Distinct Structures of Membrane‐Associated Homodimers of GTP‐ and GDP‐Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement
Lee K, Fang Z, Enomoto M, Gasmi‐Seabrook G, Zheng L, Koide S, Ikura M, Marshall C. Two Distinct Structures of Membrane‐Associated Homodimers of GTP‐ and GDP‐Bound KRAS4B Revealed by Paramagnetic Relaxation Enhancement. Angewandte Chemie International Edition 2020, 59: 11037-11045. PMID: 32227412, PMCID: PMC7395670, DOI: 10.1002/anie.202001758.Peer-Reviewed Original ResearchConceptsKRAS dimerizationParamagnetic relaxation enhancement NMR spectroscopyStructural basisΑ4-α5 interfaceEffector-binding siteParamagnetic relaxation enhancementActive GTPRaf activationSmall GTPasesDistinct structuresRaf kinaseGTPDimerizationRelaxation enhancementKRAS4bGTPasesR135E168ActivationKinaseProtomersHomodimerNanodiscsNMR spectroscopyMembrane
2016
Biochemical Classification of Disease-associated Mutants of RAS-like Protein Expressed in Many Tissues (RIT1)*
Fang Z, Marshall C, Yin J, Mazhab-Jafari M, Gasmi-Seabrook G, Smith M, Nishikawa T, Xu Y, Neel B, Ikura M. Biochemical Classification of Disease-associated Mutants of RAS-like Protein Expressed in Many Tissues (RIT1)*. Journal Of Biological Chemistry 2016, 291: 15641-15652. PMID: 27226556, PMCID: PMC4957048, DOI: 10.1074/jbc.m116.714196.Peer-Reviewed Original ResearchConceptsDisease-associated mutationsGTPase cycleIntrinsic GTP hydrolysis rateA57GRIT1 mutationsIntrinsic nucleotide exchange rateDisease-associated mutantsGTP hydrolysis rateNucleotide exchange rateReal-time NMRSmall guanosine triphosphataseHEK293T cellsPulldown assaysGuanosine triphosphataseGTP hydrolysisNoonan syndromeGTPase assaysDivergent residuesRIT1GTPase propertiesMutantsModest activationH-RASMutationsUrinary tract cancer