2024
Cross-regulations of two connected domains form a mechanical circuit for steady force transmission during clathrin-mediated endocytosis
Ren Y, Yang J, Fujita B, Zhang Y, Berro J. Cross-regulations of two connected domains form a mechanical circuit for steady force transmission during clathrin-mediated endocytosis. Cell Reports 2024, 43: 114725. PMID: 39276354, PMCID: PMC11476202, DOI: 10.1016/j.celrep.2024.114725.Peer-Reviewed Original ResearchClathrin-mediated endocytosisF-actinActin cytoskeletonFission yeast Schizosaccharomyces pombeYeast Schizosaccharomyces pombeCell adhesion complexAdhesion complexesMembrane localizationPN forcesStable bindingEnd4pCross-regulationCytoskeletonActinEndocytosisMembraneBindingMechanical forcesTalinTransmission of forcesThatchForce transmissionDomainCellsFissionIn vivo force measurements on formin Cdc12p using new coiled-coil force sensors
Saito T, Ren Y, Berro J. In vivo force measurements on formin Cdc12p using new coiled-coil force sensors. Biophysical Journal 2024, 123: 465a. DOI: 10.1016/j.bpj.2023.11.2817.Peer-Reviewed Original Research
2023
Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors
Ren Y, Yang J, Fujita B, Jin H, Zhang Y, Berro J. Force redistribution in clathrin-mediated endocytosis revealed by coiled-coil force sensors. Science Advances 2023, 9: eadi1535. PMID: 37831774, PMCID: PMC10575576, DOI: 10.1126/sciadv.adi1535.Peer-Reviewed Original ResearchConceptsActin cytoskeletonPlasma membraneHuntingtin Interacting Protein 1Clathrin-mediated endocytosisCountless cellular processesEndocytic machineryCellular processesClathrin latticesProtein condensationCytoskeletonEnd4pProtein 1Membrane deformationPiconewton forcesEndocytosisVivo force measurementsMembranePiconewtonsClathrinMachineryProteinCoatMolecular scale2A peptide from ERBV-1 efficiently separates endogenous protein domains in the fission yeast Schizosaccharomyces pombe
Ren Y, Lin Q, Berro J. 2A peptide from ERBV-1 efficiently separates endogenous protein domains in the fission yeast Schizosaccharomyces pombe. MicroPublication Biology 2023, 2023: 10.17912/micropub.biology.000941. PMID: 37767365, PMCID: PMC10520729, DOI: 10.17912/micropub.biology.000941.Peer-Reviewed Original Research
2022
Force redistribution during clathrin-mediated endocytosis is revealed by new phase-separating force sensors
Ren Y, Yang J, Jin H, Zhang Y, Berro J. Force redistribution during clathrin-mediated endocytosis is revealed by new phase-separating force sensors. Biophysical Journal 2022, 121: 230a. DOI: 10.1016/j.bpj.2021.11.1598.Peer-Reviewed Original Research In PressIsolated THATCH domain of End4 is unable to bind F-actin independently in the fission yeast Schizosaccharomyces pombe
Ren Y, Berro J. Isolated THATCH domain of End4 is unable to bind F-actin independently in the fission yeast Schizosaccharomyces pombe. MicroPublication Biology 2022, 2022: 10.17912/micropub.biology.000508. PMID: 35024575, PMCID: PMC8738963, DOI: 10.17912/micropub.biology.000508.Peer-Reviewed Original Research
2021
A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin.
Brown S, Ren Y, Suter D, Mattoo S. A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin. Bio-protocol 2021, 11: e4158. PMID: 34692908, PMCID: PMC8481015, DOI: 10.21769/bioprotoc.4158.Peer-Reviewed Original ResearchPhosphorylation of cortactinActin cytoskeletal dynamicsNon-phosphorylated proteinActin-binding proteinsRecombinant protein expressionCell-free systemTumor cell metastasisNeuronal growth conesBacterial kinasesLamellipodia protrusionBag cell neuronsPhosphorylation assaysNon-neuronal cellsFilopodia formationSrc kinaseInduced proteinsCortactinTyrosine kinaseEfficient expressionKinaseCell migrationPhosphorylationBiochemical studiesCell metastasisGrowth conesRapid adaptation of endocytosis, exocytosis and eisosomes after an acute increase in membrane tension in yeast cells
Lemière J, Ren Y, Berro J. Rapid adaptation of endocytosis, exocytosis and eisosomes after an acute increase in membrane tension in yeast cells. ELife 2021, 10: e62084. PMID: 33983119, PMCID: PMC9045820, DOI: 10.7554/elife.62084.Peer-Reviewed Original ResearchConceptsClathrin-mediated endocytosisMembrane tensionActin machineryYeast cellsFission yeast cellsRate of endocytosisRate of exocytosisActin assemblyLarge membrane tensionPlasma membraneCell wallMolecular mechanismsTurgor pressureEndocytosisRapid adaptationEisosomesExocytosisMachineryQuantitative microscopyMinute time scaleHypotonic shockCellsEukaryotesMembraneTension regulation
2019
A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones
Ren Y, He Y, Brown S, Zbornik E, Mlodzianoski M, Ma D, Huang F, Mattoo S, Suter D. A single tyrosine phosphorylation site in cortactin is important for filopodia formation in neuronal growth cones. Molecular Biology Of The Cell 2019, 30: 1817-1833. PMID: 31116646, PMCID: PMC6727743, DOI: 10.1091/mbc.e18-04-0202.Peer-Reviewed Original ResearchConceptsTyrosine phosphorylation sitesPhosphorylation sitesFilopodia formationNeuronal growth conesSingle tyrosine phosphorylation sitePhosphorylated cortactinGrowth conesPhospho-mimetic mutantVitro kinase assaysFilopodia lengthFunction of cortactinTyrosine-phosphorylated cortactinFormation of filopodiaSrc tyrosine kinaseCortactin mutantMultiple actinPhosphorylation substratesActin organizationCellular processesLength of filopodiaKinase assaysCortactinTyrosine kinaseNonneuronal cellsFilopodia
2018
Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development, Organization and Neurite Outgrowth from Human Pluripotent Stem Cells
Fligor C, Langer K, Sridhar A, Ren Y, Shields P, Edler M, Ohlemacher S, Sluch V, Zack D, Zhang C, Suter D, Meyer J. Three-Dimensional Retinal Organoids Facilitate the Investigation of Retinal Ganglion Cell Development, Organization and Neurite Outgrowth from Human Pluripotent Stem Cells. Scientific Reports 2018, 8: 14520. PMID: 30266927, PMCID: PMC6162218, DOI: 10.1038/s41598-018-32871-8.Peer-Reviewed Original ResearchConceptsRetinal ganglion cellsRetinal organoidsAxonal outgrowthRGC developmentNeurite outgrowthExtensive axonal outgrowthRetinal ganglion cell developmentPost-synaptic targetsGanglion cell developmentStem cellsPluripotent stem cellsRGC layerThree-dimensional retinal organoidsGanglion cellsElaborate growth conesRGC differentiationHuman pluripotent stem cellsCellular replacementRetinal developmentGrowth factor signalingGrowth conesCell developmentOrganoidsFactor signalingGuidance receptorsA low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D
Ren Y, Mlodzianoski M, Lee A, Huang F, Suter D. A low-cost microwell device for high-resolution imaging of neurite outgrowth in 3D. Journal Of Neural Engineering 2018, 15: 035001. PMID: 29363623, PMCID: PMC6040821, DOI: 10.1088/1741-2552/aaaa32.Peer-Reviewed Original ResearchsCMOS Noise Correction Algorithm for Microscopy Images
Liu S, Mlodzianoski M, Hu Z, Ren Y, McElmurry K, Miller D, Ziegler K, Ivey P, Ma D, Suter D, Huang F. sCMOS Noise Correction Algorithm for Microscopy Images. Biophysical Journal 2018, 114: 347a. DOI: 10.1016/j.bpj.2017.11.1936.Peer-Reviewed Original Research
2017
sCMOS noise-correction algorithm for microscopy images
Liu S, Mlodzianoski M, Hu Z, Ren Y, McElmurry K, Suter D, Huang F. sCMOS noise-correction algorithm for microscopy images. Nature Methods 2017, 14: 760-761. PMID: 28753600, PMCID: PMC6016843, DOI: 10.1038/nmeth.4379.Peer-Reviewed Original Research
2016
Increase in Growth Cone Size Correlates with Decrease in Neurite Growth Rate
Ren Y, Suter D. Increase in Growth Cone Size Correlates with Decrease in Neurite Growth Rate. Neural Plasticity 2016, 2016: 3497901. PMID: 27274874, PMCID: PMC4870373, DOI: 10.1155/2016/3497901.Peer-Reviewed Original Research
2015
Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones
He Y, Ren Y, Wu B, Decourt B, Lee A, Taylor A, Suter D. Src and cortactin promote lamellipodia protrusion and filopodia formation and stability in growth cones. Molecular Biology Of The Cell 2015, 26: 3229-3244. PMID: 26224308, PMCID: PMC4569314, DOI: 10.1091/mbc.e15-03-0142.Peer-Reviewed Original ResearchConceptsActin network densityGrowth conesSrc tyrosine kinaseFluorescent speckle microscopyState of actinNeuronal growth conesGrowth cone motilityLamellipodia protrusionActin organizationActin assemblyFilopodia formationActin bundlesAplysia growth conesSpeckle microscopyTyrosine kinaseUnderlying cellular mechanismsFilopodial densityCone motilityCellular mechanismsSrcFilopodiaActivation stateLamellipodiaSRC2Axonal growth
2012
Validation of Polo-like kinase 1 as a therapeutic target in pancreatic cancer cells
Zhang C, Sun X, Ren Y, Lou Y, Zhou J, Liu M, Li D. Validation of Polo-like kinase 1 as a therapeutic target in pancreatic cancer cells. Cancer Biology & Therapy 2012, 13: 1214-1220. PMID: 22892842, PMCID: PMC3469479, DOI: 10.4161/cbt.21412.Peer-Reviewed Original ResearchConceptsPancreatic cancer cellsCancer cellsPolo-like kinase 1Human pancreatic cancer cellsChemotherapeutic drug gemcitabinePLK1 inhibitorsCombination index analysisKinase 1Pancreatic cancerClinical investigationTherapeutic targetCancer treatmentExpression levelsDrug gemcitabineValuable targetCellsTreatmentInhibitorsProliferationCritical roleCep70 promotes microtubule assembly in vitro by increasing microtubule elongation
Shi X, Wang J, Yang Y, Ren Y, Zhou J, Li D. Cep70 promotes microtubule assembly in vitro by increasing microtubule elongation. Acta Biochimica Et Biophysica Sinica 2012, 44: 450-454. PMID: 22427462, DOI: 10.1093/abbs/gms017.Peer-Reviewed Original ResearchConceptsCentrosomal proteinsMicrotubule assemblyMajor microtubule-organizing centerNovel centrosomal proteinDynamic cytoskeletal polymersMicrotubule-organizing centerMicrotubule elongationEukaryotic cellsMammalian cellsAnimal cellsMolecular detailsCep70Microtubule nucleationMolecular mechanismsCytoskeletal polymersMicrotubule lengthProteinMicrotubule numberAssemblyCellsCentrosomesElongationMicrotubulesGekko-sulfated Glycopeptide Inhibits Tumor Angiogenesis by Targeting Basic Fibroblast Growth Factor*
Zhang S, Zhu C, Ba Y, Chen D, Zhou X, Cao R, Wang L, Ren Y, Wu X. Gekko-sulfated Glycopeptide Inhibits Tumor Angiogenesis by Targeting Basic Fibroblast Growth Factor*. Journal Of Biological Chemistry 2012, 287: 13206-13215. PMID: 22371501, PMCID: PMC3340002, DOI: 10.1074/jbc.m111.321521.Peer-Reviewed Original ResearchMeSH KeywordsAnimalsCarcinoma, HepatocellularChick EmbryoChickensDrug DiscoveryDrugs, Chinese HerbalEarly Growth Response Protein 1Fibroblast Growth Factor 2GlucuronidaseHep G2 CellsHeparinHuman Umbilical Vein Endothelial CellsHumansLiver Neoplasms, ExperimentalMaleMiceMice, NudeNeovascularization, PathologicPolysaccharidesSurface Plasmon ResonanceXenograft Model Antitumor AssaysConceptsBasic fibroblast growth factorFibroblast growth factorTumor angiogenesisProduction of bFGFEndothelial cellsGrowth factorGekko swinhonis GuentherAbsence of bFGFInhibits tumor angiogenesisXenograft mouse modelLow-affinity receptorsEarly growth response 1Traditional Chinese medicineRelease of bFGFHeparan sulfateMouse modelTherapeutic targetHeparanase enzymatic activityTumor growthBFGF productionHeparin/heparan sulfateAffinity receptorInhibitory effectChinese medicineExtracellular matrixInhibition of farnesyltransferase reduces angiogenesis by interrupting endothelial cell migration
Peng G, Ren Y, Sun X, Zhou J, Li D. Inhibition of farnesyltransferase reduces angiogenesis by interrupting endothelial cell migration. Biochemical Pharmacology 2012, 83: 1374-1382. PMID: 22382068, DOI: 10.1016/j.bcp.2012.02.008.Peer-Reviewed Original ResearchConceptsEndothelial cellsCell migrationInhibition of farnesyltransferaseTherapeutic targetTumor cellsCancer treatmentEndothelial cell migrationUse of FTIsProtein 1Cancer therapyAngiogenesisPseudopodia formationInhibition of FTaseInhibitors of farnesyltransferaseInhibitionCellsLocalization of EB1Novel insightsPhysiological processesTherapyMicrotubule‐binding protein CLIP‐170 is a mediator of paclitaxel sensitivity
Sun X, Li D, Yang Y, Ren Y, Li J, Wang Z, Dong B, Liu M, Zhou J. Microtubule‐binding protein CLIP‐170 is a mediator of paclitaxel sensitivity. The Journal Of Pathology 2012, 226: 666-673. PMID: 21989536, DOI: 10.1002/path.3026.Peer-Reviewed Original ResearchConceptsBreast cancerPaclitaxel sensitivityPaclitaxel-containing chemotherapyActivity of paclitaxelBreast cancer correlatesCaspase-3 activity analysisBreast cancer cell linesBreast cancer cellsAbility of paclitaxelCancer cell linesCell proliferation assaysCancer correlatesPathological responsePaclitaxelProliferation assaysCell cycle progressionCancer cellsClinical samplesMitotic indexCell linesCancerMicrotubule-dependent mechanismCycle progressionDrug paclitaxelMicrotubule-binding protein