A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin.
Brown S, Ren Y, Suter D, Mattoo S. A Co-purification Method for Efficient Production and Src Kinase-mediated Phosphorylation of Aplysia Cortactin. Bio-protocol 2021, 11: e4158. PMID: 34692908, PMCID: PMC8481015, DOI: 10.21769/bioprotoc.4158.Peer-Reviewed Original ResearchPhosphorylation of cortactinActin cytoskeletal dynamicsNon-phosphorylated proteinActin-binding proteinsRecombinant protein expressionCell-free systemTumor cell metastasisNeuronal growth conesBacterial kinasesLamellipodia protrusionBag cell neuronsPhosphorylation assaysNon-neuronal cellsFilopodia formationSrc kinaseInduced proteinsCortactinTyrosine kinaseEfficient expressionKinaseCell migrationPhosphorylationBiochemical studiesCell metastasisGrowth cones